Biomedical subjects

M Tigałonowa

Publications and source records attributed to M Tigałonowa.

10 recordsLinked to original sources

PubMed1989

Effects of psoriatic patients' neutrophils and sera on angiogenic capability and natural cytotoxicity of normal human peripheral blood mononuclear cells.

We studied the effects of products released from adhering neutrophils and serum factors from patients with psoriasis on angiogenic capability and natural cytotoxicity of normal human mononuclear cells. In active disseminated and guttate psoriasis, in which polymorphonuclear granulocytes were most adherent to the human vascular endothelium, supernatants from adhering neutrophils only slightly affected natural killer cell activity and angiogenic capability of normal human mononuclears. In peripherally spreading, plaque-like psoriasis, characterized by lower than in guttate psoriasis increase in granulocyte adherence, both neutrophil factors and patients sera markedly decreased NK cell activity and enhanced angiogenic capability of normal human peripheral blood mononuclear cells. The results suggest that neutrophil factors may affect some immune responses in patients with psoriasis.

Adolescent↗

PubMed1988

Alpha 1-proteinase inhibitor in psoriasis: reduced activity in symptom-free patients and during flare.

The aim of this study was to quantitate the active fraction of the alpha 1-proteinase inhibitor (alpha 1-PI) in psoriasis. Serum proteinase inhibitory capacity was measured vs porcine pancreatic elastase of a known active fraction against its specific substrate (Suc-Ala3-pNA). The inhibitory capacity was determined in 21 symptom-free patients, 134 patients with skin lesions, and 23 healthy volunteers. Alpha 1-PI was found to be significantly decreased in symptom-free patients and in those with stationary lesions, in a manner similar to the reduced activity of neutrophil proteinases, elastase, and cathepsin G. The synthesis of alpha 1-PI was stimulated during the appearance of active psoriatic lesions, but to a much lesser degree in patients with early onset (less than or equal to 21 years) than in patients with late onset of psoriasis (greater than 21 years). The early onset subgroup differed by a more frequent familial occurrence of psoriasis and a more severe course of the disease. The data indicate that the regulation of the proteinase-alpha 1-PI system in psoriasis is abnormal and this may contribute to the pathogenesis of the disease. The decreased alpha 1-PI during flare may be responsible for the disease activity, at least in patients with early onset of psoriasis.

Adolescent↗

PubMed1985

Leukopheresis for treatment of psoriasis: is therapeutical benefit related to reduced activities of neutral proteinases of polymorphonuclear leukocytes?

Ten patients were treated with repeated leukophereses performed one to three times per week for 2-5 weeks. Two of the patients was cleared completely, four exhibited regression of more than one-half of the lesions, and four showed only a slight improvement. The therapy did not markedly affect the granulocyte count in peripheral blood, and the beneficial clinical response was not related to the number of polymorphonuclear leukocytes (PMNs) removed by leukophereses. During therapy, the activities of elastase, cathepsin G, lysozyme, and myeloperoxidase in PMNs were determined by spectrophotometry. PMNs isolated using a Haemonetics 30 blood-cell separator were about 50% deficient in these activities in comparison to cells obtained directly from peripheral blood. Thus, leukopheresis induces a marked degranulation of PMNs. Repeated leukophereses were found to generate significant variations in the activities of circulating PMN granule enzymes and in the levels of acid-soluble proteins. Remission or great improvement were observed in patients who, during therapy, exhibited decreased PMN elastase and cathepsin G activities, whereas a poor clinical response was accompanied by high enzymatic activities.

Adult↗

PubMed1980