Biomedical subjects
Publications and source records attributed to M Tobien.
A pilot study examined the frequency spectrum of TMJ-sounds, in particular clicking. A profound difference was observed between articular movement noises and clinical clicking. The frequency spectrum of normal movements is clearly different from the one observed for clicking. Therefore phonognathography might be an additional diagnostic tool to elucidate the reasons for individual clicking phenomena with Fourier analysis.
Explore the source record for details and available documents.
DNA-dependent RNA polymerase core enzyme was isolated from Halobacterium halobium. The purification is based on the finding that the enzyme is stable in 40% (v/v) glycerol, in the presence of 0.05 M MgCl2 and involves adsorption of contaminants to DEAE-cellulose, precipitation of the complex of polymerase with DNA by streptomycin sulfate, chromatography over Biogel and affinity chromatography over heparin-Sepharose or heparin-cellulose. The enzyme consists of four or five different subunits. The composition formula was estimated as (150000) (86000)2 (72000)2 (49000)3 or 2; there may be one or two different 49000-Mr subunits. RNA synthesis requires a template. Denatured DNA is more efficient than native DNA. The transcription of native DNA is specifically stimulated by the addition of a possibly sigma-like factor eluted from DEAE-cellulose. The fidelity of transcription is indicated by the absolute requirement for UTP besides ATP with poly[d(A-T)] as the template.
Explore the source record for details and available documents.