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M Todokoro

Publications and source records attributed to M Todokoro.

7 recordsLinked to original sources
PubMed1999

Preparation of poly(epsilon-lysine) adsorbents and application to selective removal of lipopolysaccharides.

To remove endotoxins (lipopolysaccharides; LPS) from cell products used as drugs, water-insoluble poly(epsilon-lysine) (PL) particles were prepared by cross-linking with PL originating from Streptomyces albulus and chloromethyloxirane (CMO). The apparent pKa (pK(a,app)) and the anion-exchange capacity of the particles were easily adjusted by changing the PL ratio and the CMO ratio. The higher the pK(a,app), the greater the LPS-adsorption capacity of the particles. On the other hand, when the PL ratio (in the particles) increased to 75 unit-mol% or higher, the adsorption of bovine serum albumin by the particles also increased, but decreased with increasing ionic strength of the buffer to mu = 0.2 or higher. The adsorption of gamma-globulin increased with decreasing PL ratio to 65 unit-mol% or lower. As a result, when the PL ratio was 70 unit-mol% and the pK(a,app) was 6.7, the PL/CMO particles selectively removed LPS from various protein solutions that were naturally contaminated with LPS, at pH 6.0 and mu = 0.05.

Adsorption↗
PubMed1975

Chrysanthemum pollinosis in Japan.

Using the scratch test with self-made chrysanthemum pollen extract of 32,700 PNU/ml, a positive response was elicited in 60 of 316 patients (18.9%) with allergic rhinitis and bronchial asthma, and was positive in 42.5% adults with allergic rhinitis. On the other hand a 4.7% positive response was obtained in 84 non-allergic subjects. With the intracutaneous test, a threshold value was determined in 7 cases with 0.327 PNU/ml; 12 with 3.27 PNU/ml; 8 with 32.7 PNU/ml; and 8 with 327 PNU/ml, as opposed to one positive in 84 controls with 327 PNU/ml (1.3%). P-K tests were successfully done in 15 out of 16 cases. Furthermore, the results of in vitro neutralization tests using chrysanthemum and other compositae pollen extracts indicated the absence of sharing antigenic determinants between them. Provocation tests were conducted in 4 cases of allergic rhinitis and 3 cases of bronchial asthma with positive response in all the patients. From these results it was found that chrysanthemim pollinosis indeed exists in Japan, particularly in the mountainous districts.

Adolescent↗