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Biomedical subjects

M Tolonen

Publications and source records attributed to M Tolonen.

At least 19 recordsLinked to original sources
PubMed2003

Production of nisin with continuous adsorption to Amberlite XAD-4 resin using Lactococcus lactis N8 and L. lactis LAC48.

The production of nisin, biomass and lactic acid in pH-controlled and uncontrolled batch fermentation and batch fermentation (pH 5.5) with continuous removal of nisin was examined in the parent strain Lactococcus lactis N8 and LAC48. Strain LAC48 in batch fermentor (pH not controlled) gave a maximum nisin concentration of 2.5 x 10(6) IU g dcw(-1). The nisin concentration remained high (2.0 x 10(6) IU g dcw(-1)) after the logarithmic growth phase (10-22 h), whereas nisin production of strain N8 decreased after the logarithmic growth phase. The maximum nisin production of strain LAC48 was not directly related to the biomass formation and not associated with growth. In order to study end product inhibition in nisin production, a system was built for adsorption of nisin during fermentation. The adsorbent Amberlite XAD-4 was found to have an effective binding capacity for nisin. Cells of LAC48 and N8 compensated for the removal of nisin, indicating that nisin production also occurs in the stationary phase.

Adsorption↗
PubMed1988

Vitamin B6 status of Finnish elderly. Comparison with Dutch younger adults and elderly. The effect of supplementation.

About 25% of Finnish and Dutch elderly appeared to be more or less deficient in vitamin B6 as compared to younger adults. Deficiency was observed at the cellular (PLP, EGOT and alpha-EGOT) as well as at the plasma level (PLP). The benefit of a one-year daily supplementation with 2 mg of pyridoxine-HCl was investigated at the biochemical and psychological level as compared to a placebo group. After one year, none of the supplemented elderly was deficient in biochemical terms. At the psychological level and at the level of general well-being, the elderly supplemented with vitamin B6 showed slight improvements. However, for the psychological variables significant correlations with the vitamin B6 parameters were not observed. Plasma fatty acids (e.g. gamma-linolenic acid) showed no correlation with the vitamin B6 status.

Aged↗
PubMed1983

Selenium levels in whole blood of Finnish volunteers before and during organic and inorganic selenium supplementation.

High selenium barley biscuits containing 1 mumol (70 micrograms) organic Se were administered to healthy male volunteers for 5 weeks at doses of 2.1 mumol Se (group A) or 6.4 mumol (group B). In addition, 2 mg Na-selenate capsules (5.4 mumol Se) were given to two other groups at daily doses of 2 mg (group C) or 8 mg (group D). Groups A, B and C each comprised eight healthy men and group D eight healthy women and three men. The initial median concentration of whole blood selenium (B-Se for groups A, B and C were 1.0-1.1 mumol/l (range 0.7-1.7) and for group D 1.3 mumol/l (range 0.9-1.8). In 1-2 weeks time the B-Se concentrations rose to 1.6 mumol/l for groups A and C, to 1.8 mumol/l for group B, and to 2.2 mumol/l for group D. There was no decrease 1 week after the Se intake ceased. As expected, the level of B-Se increased more (in relation to dose) in those given organic Se than in those given inorganic Se. Groups A, B and C, however, had rather moderate increases. The daily dose required to raise the B-Se of Finns up to the North American level (2.2 mumol/l) was as high as 8 mg Na-selenate (21.5 mumol or 1700 micrograms Se), but the dose of organic Se which would be required to achieve this level is not yet known.

Female↗
PubMed1982

Quantitated effects of carbon disulfide exposure, elevated blood pressure and aging on coronary mortality.

Risk function analysis was performed in order to clarify the relationship between coronary heart disease death rate and occupational carbon disulfide exposure along with two other important risk factors, elevated diastolic blood pressure and older age, in a data set obtained from a 10-year prospective follow-up of coronary mortality in two cohorts of over 340 male industrial workers in Finland. The sole effect of carbon disulfide on the coronary mortality was statistically significant (relative risk = 2.3), and it was largely independent of the level of the other two risk factors. Log-linear models were fitted to predict coronary heart disease deaths. The analysis identified the dual role of raised blood pressure in the potential mechanism of coronary death. Relative risks ranging up to 48 were obtained for the joint non-synergistic effect of these three factors in the exposed group compared to a subgroup of nonexposed, 40-year-old normotensive men. The effect of carbon disulfide exposure on the risk of coronary death, while clearly distinguishable from the effect of hypertension and aging, stayed subordinate to them, but nevertheless remained an important risk element in the work environment.

Adult↗
PubMed1981

Impaired color discrimination among viscose rayon workers exposed to carbon disulfide.

A possible effect of chronic carbon disulfide exposure on the optic nerve was studied by giving the Farnsworth Munsell 100-Hue Test for color discrimination to 62 exposed and 40 nonexposed men. Carbon disulfide exposure did not relate to specific pattern defects in color discrimination, but impaired color discrimination occurred significantly more often in the exposed group than among the referents. The abnormal findings suggest an impairment in the receptiveness of the ganglion cells or demyelination of the optic nerve fibers.

Adult↗
PubMed1979

Ten-year coronary mortality of workers exposed to carbon disulfide.

Two cohorts, one comprising 343 viscose rayon workers exposed for at least five years to carbon disulfide (CS2) and the other made up of 343 nonexposed men, were followed from 1967 to 1977 with respect to coronary heart disease (CHD) mortality. In the examination in 1967 known risk factors of CHD were controlled; only blood pressure was slightly higher in the exposed group. The difference was considered a result of exposure. Five exposed and eight nonexposed men had experienced a previous clinical myocardial infarction. The total mortality was 48 (14%) in the exposed group and 31 (9%) in the compeer group (p congruent to 0.05); 29 exposed and 11 nonexposed men had died from CHD (p less than 0.01), and 5 exposed and 1 nonexposed from other cardiovascular causes (p = 0.1). All coronary deaths occurred in the age interval 40 to 69 years. The estimated risk of death from CHD for this 30-year age span, assuming no competing risks of death, was 31.9% for the exposed cohort against 13.3% for the compeers. A multivariate analysis yielded age, raised blood pressure, and exposure to CS2 as prominent risk factors. The contributory risk caused by past occupational CS2 exposure seemed to increase the already notoriously high risk of CHD mortality among Finnish men.

Adult↗
PubMed1979

Municipal occupational health services for small workplaces. Background and general methodology of the study.

This paper deals with the new Occupational Health Service Act of Finland, as well as the background, scope and general methodology of a walk-through survey. The study comprised 163 small places of work within two municipal health center areas, and the primary objective was to determine their need for actual occupational health services. The workplaces were surveyed for a comprehensive picture of their physical and chemical hazards, as well as for knowledge of first-aid preparedness, need for job-related health counseling, personnel facilities, personal protection, and the required ergonomic and safety activities at the workplaces within the community. This ad-hoc information was considered essential for the planning of a nationwide occupational health program, and, more specifically, an assessment could be made of the utility of health personnel in reducing and preventing occupational health and safety risks at small places of work. More specific details of the sample and methodology are reported in other communications.

Absenteeism↗
PubMed1979

First-aid preparedness in small workplaces with special reference to occupational health services.

First-aid preparedness was surveyed in 163 small firms employing 2,400 persons. Although 11% of the employees had received first-aid training, half of the firms lacked first-aiders. Only one-fourth of the first-aid kits were equipped according to instructions. Checking and maintenance of the equipment, as well as information about first aid at the place of work, were generally inadequate. Instructions were commonly lacking for the safe use of chemical substances. Only 9% of the firms fully complied with the requirements for optimum first-aid preparedness. Employers at small firms seem not to be sufficiently aware of their liabilities with regard to first aid. Yet, most of the faults and defects could be corrected by means of arrangements within the firms once the instructions were better known. During their visits to places of work occupational health personnel should inspect the first-aid preparedness and advise the employer on first-aid requirements. In addition the expertise of the insurance company, the local Red Cross or the regional institute of occupational health may be utilized.

Finland↗
PubMed1979

Personnel facilities in small workplaces with special reference to occupational health services.

The personnel facilities of small workplaces generally complied with the requirements. Yet, defects were detected in 20% of the 163 firms surveyed. Most of the faults were insignificant from the point of view of health. The failures were due to the fact that the facilities had not originally been planned for their present purpose. The need for the inspection of sanitary installations such as toilets and washrooms and facilities for storing and drying clothes was noted in dirty jobs and in places of work with toxicologic hazards. The management of small firms rarely needs medical advice on sanitary, catering and welfare amenities.

Finland↗
PubMed1979

Need for job-related health counseling in small workplaces.

Health counseling aimed at maintaining health and safety on the job is required in most small workplaces. From a labor protection point of view, the most important advice deals with environmental hazards such as protection against physical and chemical factors, ergonomic problems, and safety risks. Information provided by the employers was considered insufficient in three-fourths of the 163 firms surveyed in this study. The staff providing local occupational health services could perform two broad types of counseling. First, they could educate management as to its responsibilities for the health and safety of employees; health personnel can instruct management during their workplace visits. Second, during health examinations and other personal contacts, the worker can be advised about ways to improve or maintain his health. Moreover, such occasions also provide an opportunity for more extensive health counseling about personal, social emotional, and other problems although, in municipal health centers, these subjects belong within the scope of public health rather than occupational health.

Accidents, Occupational↗
PubMed1979

Need for protective clothing and equipment in small workplaces.

Some kind of protective clothing and personal safety items was needed in 90% of the 163 workplaces surveyed. Protective clothing was used in 31%, ear protectors in 28%, eye protectors in 22%, respiratory tract protectors in 18% and other safety items in 1% of the firms. Gloves were the most commonly used type of protective clothing. Protection against risks was proper in one-fourth of the firms, satisfactory in half, and poor or lacking in the rest. Ninety-five percent of the protectors available were appropriate, but only in a few companies were they properly cared for. Seven (12%) industrial firms, 15 (20%) service establishments and 1 (7%) other firm lacked proper protective clothing or safety items. Faults were generally attributed to insufficient knowledge or negligence of responsibilities on the part of the employers. Management should provide more appropriate instruction for workers in the use and, particularly, the maintenance of personal protectors. To be effective, the inspection of the appropriateness of the protectors requires suitable training. This training should also be received by the staff providing occupational health services. Visits to workplaces, as well as health examinations, provide an opportunity to discuss protection and to advise employers and employees on protection. The problems of protection against harmful exposures is closely connected with health counseling. Yet, the provision of proper protectors and the supervision of their use is still the responsibility of the employer.

Accidents, Occupational↗