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Biomedical subjects

M Triller

Publications and source records attributed to M Triller.

At least 19 recordsLinked to original sources
PubMed1987

Artificially induced developmental defects in sheep enamel examined by scanning electron microscopy.

Upper incisor teeth from sheep infected with parasites or given 6 mg fluoride per kg bodyweight for 21 days during the period of tooth formation were examined with SEM and microradiography. It was found using both techniques that there had been a severe disturbance of the secretory ameloblasts leading to enamel hypoplasia. It is suggested that it is the severity rather than the cause of the insult to the ameloblasts which determines the degree of hypoplasia.

Ameloblasts↗
PubMed1981

[In vitro dentinogenesis of mice fetuses tooth germs in the presence of fluoride. Morphological nad historical observations].

Observations show that fluoride alters dentinogenesis in vitro. Morphological and cellular alterations are greater when the tooth germs are young and/or when the time of incubation is longer and the dose of fluoride higher. In the predentine, the stainability of proteoglycans is partially reduced. The persistence of alcianophilic zones far from the site of secretion indicates some delay of the process of matricial maturation.

Animals↗
PubMed1979

Structural and histochemical observations of fluorotic enamel matrix.

The matrix of fluorotic enamel is studied by S.E.M. and histochemically by light microscopy. Observations are made on fluorotic human teeth and on rat incisors in which fluorosis has been produced by daily injections of NaF. The enamel sheaths appear enlarged and irregular, rich in stainable proteins, while glycocomponents of the matrix are less dense. A relationship is suggested between those alterations and the further irregular pattern of mineralization of fluorotic enamel.

Animals↗
PubMed1979

Comparative investigations on fluorosed enamel.

Structural observations upon fluorosed enamel were made on human teeth extracted from Nabeul (Tunisia). Longitudinal sections of enamel were made and microradiographed. Topographical observatoons in the S.E.M. showed alterations of the pattern of mineralization. Fissures and clefts filled with plaque material were observed to reach to the surface of the teeth. They penetrated into enamel along the prisms. Acid etching of the surface by 30% H3PO4 revealed enlarged acid-resistant sheaths while the core of the prisms was irregularly etched. Inner portions of the enamel were observed on fractured fragments. The structure was not significantly perturbated except for areas of porosity. After demineralization of the sections by 10% EDTA at pH 7 and critical point drying, the organic matrix was observed in the S.E.M. : It appeared as a fibrous network. Compared with sound teeth the matrix was dense and irregular. Histochemical stainings showed the presence of organic material. Alcian blue and PAS positive contents were very low except near the enamel-dentine junction. Preliminary observations were made using SMI Cameca ion microanalyser and histochemical studies on undecalcified sections were made in order to compare the observations made with light microscopy with S.E.M. findings.

Acids↗
PubMed1976

[Acid mucopolysaccharide detection on ultra-thin sections by alcian blue in dental tissues embedded in Epon].

Ultra-thin sections of dental tissues, fixed in glutaraldehyde and osmium tetroxide, embedded without previous demineralization in Epon, were contrasted the grids with an alcian blue solution after previous oxidation periodic acid or hydrogen peroxide. Correlations with histochemical results obtained with the optical microscope, other results obtained with ruthenium red and phosphotungstic acid and controls made after enzymatic digestion with chondroitinase AC suggest that this technique is specific for the detection of acid mucopolysaccharides in those calcified tissues studied.

Animals↗