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Biomedical subjects

M Tudja

Publications and source records attributed to M Tudja.

12 recordsLinked to original sources
PubMed2002

Gizzerosine-induced histopathological lesions in broiler chicks.

1. The aim of this study was to investigate pathomorphological changes in broiler chicks fed with different doses of gizzerosine, a substance produced during the heat treatments of fish meal. 2. The experiment was carried out in Ross broiler chicks which were divided into three groups: group A received 100% of non-medicated commercial mash for broiler chicks. During an experimental 5-d period, 50% of commercial mash was replaced with unheated fish meal (0.65 ppm gizzerosine) in group B and in group C with heated fish meal (1.15 ppm gizzerosine). Fourteen chicks from each group were killed every day. Samples of gastrointestinal and lymphoid organs, lung, pancreas, liver, brain and kidney tissue were sampled for histopathological analysis. Organs were embedded in paraffin and stained with hematoxylin-eosin stain and using periodic acid-Schiff reagent (PAS) and Sudan III (frozen sections). 3. Necropsy did not reveal notable differences between treated groups. There were no significant histopathological changes in immunocompetent organs nor in the lungs, the pancreas, the kidney or the brain. Sharply demarcated multiple vacuoles were observed in the myocardium in group C toward the end of the experiment. In group C, the prevalent changes in the gizzard and the proventriculus were slight to severe cuticle erosions and oedema of the lamina propria with or without multiple vacuoles, respectively, towards the end of the experiment. The most prominent changes toward the end of the experiment were dispersed cell vacuolisation in duodenal, jejunual, ileal and caecal lamina propria in group C. 4. In conclusion, it should be emphasised that extra-gizzard gizzerosine-induced lesions are probably not mediated by H2-receptor stimulation, but could be a consequence of cellular hypoxia.

Animal Feed↗
PubMed2001

Natural zeolite clinoptilolite increases the concentrations of sphingoid bases in the yeast Yarrowia lipolytica.

In the present paper, we studied the effect of natural zeolite clinoptilolite on sphingolipid metabolism in the yeast Yarrowia lipolytica. We also investigated if zeolite addition had any impact on cell shape and size, as well as on the pH alterations during the culture growth. High performance liquid chromatography analysis of sphingoid bases obtained by acid hydrolysis of complex sphingolipids from Y. lipolytica showed that their concentrations markedly rose upon the zeolite addition. The largest increase among the identified molecular species of sphingoid bases was seen in C18 phytosphingosine, whose levels rose 6.2-fold and 22.3-fold after culturing cells for 24 and 36 hours respectively in the presence of finely ground zeolite. pH measurements of the culture medium showed a similarity between pH profiles of control and zeolite-supplemented cells, suggesting that ion-exchange capacity was not probably responsible for the observed change in sphingolipid metabolism. Scanning electron microscopy revealed that zeolite affected cell size and shape. Y. lipolytica cells grown in the absence of zeolite were oval-shaped with an average cell size of 0.7-2.7 microns, whereas when cultured with zeolite, they were round-shaped and larger, having an average cell size of 1.3-2.9 microns.

Culture Media↗
PubMed2000

Effect of amine fluoride on enamel surface morphology.

In this in vitro study, examination of the enamel surface morphology after topical application of an amine fluoride solution with different fluoride (F) content was carried out. Sound human enamel slabs were treated with an amine fluoride solution containing either 1.0, 0.5 or 0.25% F for 3 min. during 3 days. All slabs were examined using scanning electron microscopy and energy-dispersive spectroscopy (SEM/EDS) qualitative analysis. The globular precipitates were revealed in all treated specimens, regardless of F content. The distribution of the deposits was more homogeneous in groups treated with higher concentrations; however, the globules were larger and more cubical in groups treated with lower fluoride concentrations. These larger globules could be less soluble and thus serve as a fluoride reservoir for a more extended period and so they could contribute to the caries preventive effect in professional topical products with lower fluoride concentration. Following the 24-hour treatment with KOH the precipitates could be removed; however, the enamel surfaces covered with the precipitates were less degraded than the untreated enamel. The EDS qualitative analysis showed that the intensities of fluoride signals were increased with the higher concentration of fluoride in an amine fluoride solution, while the intensities of calcium signals were decreased. The enamel surface precipitates were alkali-soluble, but we were not able to demonstrate that they are pure calcium fluoride.

Calcium↗
PubMed1999

Paracetamol-propyphenazone interaction and formulation difficulties associated with eutectic formation in combination solid dosage forms.

Polymorphic behaviours of paracetamol and propyphenazone and interaction between these two compounds were investigated using differential scanning calorimetry (DSC), X-ray powder diffraction and Fourier transform-infrared (FT-IR)-spectroscopy. Binary mixtures containing various ratios of the compounds were prepared as physical and fused mixtures and analysed by DSC to study their thermal behaviours. Phase diagrams obtained from the melting endotherms of the binary mixtures demonstrated formation of an eutectic mixture at a paracetamol-propyphenazone combination of about 35:65 (w/w) with an eutectic temperature of 56 degrees C. The FT-IR spectroscopy revealed no chemical interaction due to eutectic formation, and a lower degree of crystallinity of the eutectic mixture than individual substances was observed by X-ray powder diffraction analysis. The DSC and X-ray powder diffraction data demonstrated a polymorphic change in propyphenazone as a result of melting of the compound. Tablets, containing both paracetamol and propyphenazone in a combination formulation and prepared using standard wet granulation technology, were found to have physical instability when packed in either polyvinylchloride// aluminium or polyvinylchloride/polyvinyldienechloride// aluminium blisters and stored for one month at 40 degrees C with either 75% relative humidity or without any humidity control. The instability of the tablets was more apparent under the high humidity condition.

Acetaminophen↗
PubMed1993

Temperature and surface changes of dentine and cementum induced by CO2 laser exposure.

The thermal effects of a CO2 laser on the external root surface and inside the root canal were studied in vitro by means of computerized infrared (IR) thermography and a digital thermometer. One-hundred-and-eighty tooth roots with single root canals were irradiated internally and externally with laser power set at 0.5, 1, 1.5, 2, 3 and 4 W. The laser was used in two operating modes: pulsed (pulse 0.5 s) and continuous mode with exposure time of 10 s. Under the conditions of this experiment, temperature rises of between 1.5 and 19.1 degrees C at the external root surface and 1.5 and 12 degrees C inside the root canal and horizontally across the sectioned root surface were recorded. The results obtained with IR camera showed a higher temperature on the external root surface than the digital thermometer during and after lasing the root canal. Scanning electron microscope (SEM) analysis revealed that even low laser energy significantly damaged the external root cementum surfaces.

Body Temperature↗
PubMed1992

Laser induced molar tooth pulp chamber temperature changes.

Temperature changes in enamel tissue and the pulp chamber under the influence of a CO2 laser were measured by direct methods in vitro. X-ray diffraction analysis revealed alpha-Ca3(PO4), the high-temperature modification of enamel hydroxyapatite, thus indicating that the enamel melting temperature was above 1,000 degrees C in the interaction area of laser (continuous wave, 15 s exposure time, 1 mm spot size) and tissue. Powers of 0.5 and 1 W (continuous wave), 1.5 mm spot size, and 10 s exposure time vaporize and carbonize dentin tissue at the cavity bottom of class I preparation molars. The observed temperature rise of 4 degrees C indicates that thermal injury to the pulp tissue does not occur.

Body Temperature↗
PubMed1991

[Composite retention area produced by CO2 laser beam].

Laser effect on enamel is manifested as consequence of temperature changes. Enamel melts, vaporises and a crater, and fissures are formed. Retention surface for anchoring of composite resin was obtained in intact approximal surfaces of premolars with CO2 laser (0.5, 1 and 3 W, sport size 1 and 1.5 mm, 0.2 and 0.5 s). The study results reveal composite and lased enamel interfaces are stronger then composite and inlased enamel (p less than 0.01) but weaker than composite and acid pretreated enamel interface (p less than 0.01).

Acid Etching, Dental↗
PubMed1988

[Scanning electron microscope study of early bacterial penetration of human enamel in initial caries].

Bacterial penetration of enamel during initial manifestations of the carious process was studied in intact buccal enamel of 8 impacted third permanent molars. After cleaning them from organic plaque, each buccal plane was cut into five segments, one of them serving as a control specimen and the other four being fixed into slots on partial prostheses of our volunteers. The specimens were left in oral cavity for 7, 14, 21 and 28 days, where they were exposed to the action of cariogenic factors. After removal of the organic plaque, the specimens were broken in two and the bacterial penetration into enamel was observed on the fractured cross-section using scanning electron microscope. In 4 specimens from the group orally exposed during a 7-day period, individual coccoid or bacilliform bacteria were found to have penetrated 5-10 microns deep. The number of bacteria and the depth of their penetration into enamel increased with the duration of oral exposition. Among the specimens orally exposed during 28 days, bacteria were observed to be present both individually and in colonies, penetrating to the depth of 60-90 microns. These bacteria could quite easily communicate with saliva and plaque via the pore system, which allowed them to produce metabolites, including lactic acids. This, in turn, allowed them to penetrate through the enamel, thus altering both the course and rate of the carious process progression.

Bacteria↗