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M Uchimura

Publications and source records attributed to M Uchimura.

At least 37 records · Page 2Linked to original sources
PubMed1991

An improved ELISA system for the measurement of IgG antibodies against pertussis toxin (PT) and filamentous hemagglutinin (FHA) in human sera.

We have developed a modified assay method for the measurement of antibodies against PT and FHA. Polystyrene balls coated with purified PT and FHA were incubated with standard or test sera, washed, incubated with goat anti-human IgG F(ab')2 conjugated with horseradish peroxidase and then washed again. Peroxidase activity was then measured by color development using o-phenylenediamine. The anti-PT and anti-FHA IgG titers were determined using calibration curves. The assay standard curves for anti-PT and anti-FHA were both linear from 1 to 100 ELISA units per milliliter (EU ml-1), and the assay sensitivity was calculated to be 1 EU ml-1 in both cases. Intra- and inter-assay CVs were both less than 10%. Inter-laboratory assay CVs calculated from the results from four different facilities were 3.3-34.6% (average 14.5%) and 2.1-30.3% (average 13.6%) for anti-PT and anti-FHA IgG, respectively, and the correlation coefficient between the Chinese hamster ovary (CHO) cell assay and the anti-PT PS-ball ELISA assay was 0.959. Using this assay method we obtained in a highly sensitive and reproducible measurement of IgG antibodies against PT and FHA in human sera. This assay should be especially useful for the determination of low concentrations of these antibodies.

Adhesins, Bacterial↗
PubMed1991

[Immunohistochemical studies on a new antigens associated with squamous cell cancer in oncogenesis of uterine cervical cancer].

We examined the localization of squamous cell cancer associated antigens (SCCAA) in dysplasia, cancer in situ (CIS) and microinvasive SCC of the uterine cervix, since detection of SCCAA in these subjects is highly effective for early diagnosis. Anti-squamous cell cancer associated antibody (Anti SCCAAb IgG) was prepared by immunizing rabbits with specific components at around PI 6.1 that were originally purified from SCC of maxillary sinus. In this study, the following results were obtained by the immunoperoxidase method. (1) Eleven out of 15(73%) cases of dysplasia, 20 out of 26(77%) cases of CIS, 21 out of 24(88%) cases of Stage I, 13 out of 14(93%) cases of Stage II and 9 out of 10(90%) cases of Stage III-IV in the clinical stages of SCC showed positive staining, while controls of unrelated SCC were almost negative. (2) The SCCAA positive ratio was 2 out of 2 cases of small cell nonkeratinized type, 86% in large cell nonkeratinized type and 94% in Keratinized type of SCC. (3) The SCCAA was demonstrated on all the layers of stratified squamous epithelium in a lesion of CIS and some layers migrated to adjacent nonneoplastic lesion with lateral invasion in middle layer. These results suggest that the demonstration of SCCAA may be useful in diagnosing the malignant transformation of squamous epithelium in the early stage of SCC.

Antigens, Neoplasm↗
PubMed1990

[Microtiter enzyme-linked immunosorbent assay for diphtheria antitoxin in human sera--application of parallel line assay method].

The antibodies against Diphtheria toxin in human sera were measured by the ELISA method and the antitoxin titer was calculated using the parallel line assay method which is a quantitative bioassay method. The reproducibility of this method and the comparison of the titer between this method and cell culture method were proven. The dose response curve of standard antiserum and test serum calculated by the parallel line assay method showed linearity, and the regression line of the test serum was parallel to the standard serum. The coefficients of variance (CV) of the antitoxin titers obtained from triplicate measurements of 3 serum samples ranged from 9.1 to 36.0%. The serum at low titer gave a higher CV level. A good correlation was observed between the Diphtheria antitoxin titer by the neutralizing test in cultured cells and that measured by ELISA by the parallel line assay method. The coefficient of regression was 0.996 and the coefficient of the correlation was 0.899.

Adolescent↗
PubMed1990

[A case of bone and lung metastasis of breast cancer successfully treated with radiotherapy, chemotherapy and endocrine therapy].

A 34-year-old female patient with breast cancer metastasizing to bone had a remission after a course of treatment with medroxyprogesterone acetate (MPA) 1,200 mg/day, but again had a metastasis to the lungs. The patient was then instituted on a combination therapy with UFT 400 mg/day, and MPA 1,200 mg/day. As a result, the pulmonary metastasis disappeared, along with the consolidation of the bone involved.

Adenocarcinoma↗
PubMed1988

Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels.

Formalin-fixed human small intestinal mucosa possessing villi and lymphoid follicle epithelium of Peyer's patches at the mucosal surface was used to test the adherence ability of clinically isolated strains of Vibrio cholerae O1. V. cholerae O1 grown on CFA agar for approximately 3 h at 37 degrees C had various levels of cellular hemagglutinins (HAs) and manifested adherence abilities that were roughly correlated with the cellular HA levels, irrespective of cellular HA types. V. cholerae O1 adhered better to epithelium over ileal lymphoid follicles than to epithelium of jejunal or ileal villi. Cells of different morphology which constituted lymphoid follicle epithelium were almost equal targets for adherence. In contrast, V. cholerae O1 grown on CFA agar for approximately 20 h at 37 degrees C in many cases had lower levels of cellular HAs and adherence abilities. Contrary to the above observations with cellular HAs and adherence, piliation of V. cholerae O1 was rather more extensive at approximately 20 h of incubation at 37 degrees C than at approximately 3 h of incubation at 37 degrees C. L-Fucose inhibited adherence to a varied extent depending on the cellular HA types, while D-mannose enhanced adherence in some strains. Heating of V. cholerae O1 diminished adherence ability. This adherence model system provides a tool by which various V. cholerae O1 strains can be preliminarily tested for adherence ability and site in human small intestine.

Animals↗
PubMed1987

Coexistent carcinoma in congenital dilatation of the bile duct and anomalous arrangement of the pancreatico-bile duct. Carcinogenesis of coexistent gall bladder carcinoma.

From 1972 to 1985, 40 cases of congenital dilatation of the bile duct (CDBD) were experienced in the department of the authors. Those consisted of 19 cases of anomalous arrangement of the pancreatico-bile duct (P-B anomaly), five of bile duct carcinoma, and two of gall bladder carcinoma. In addition, four patients who showed no dilatation of the bile duct in spite of the presence of a P-B anomaly were experienced, and coexistent gall bladder carcinoma was present in three of four cases. The incidence of bile duct carcinoma associated with CDBD is very high. However, coexistent gall bladder carcinoma in CDBD is a new topic, and coexistent intrahepatic bile duct carcinoma in CDBD is extremely rare. This report presents interesting and rare cases of coexistent carcinomas in these anomalies and investigates their carcinogenesis, particularly that of gall bladder carcinoma.

Adenocarcinoma, Papillary↗
PubMed1985

[Morphological study of carcinoma of the gallbladder: its differences between calculous and acalculous carcinoma].

The most characteristic phenomenon in patients with carcinoma of the gallbladder is frequent coexistence of gallstones. In this study, morphological differences of carcinoma of the gallbladder in between calculous and acalculous cases is investigated. This study involved fifty-three cases (eleven early carcinoma and forty-two advanced) that had been surgically treated at the Hamamatsu Medical Center Hospital during the past ten years. Nine of 11 early carcinomas were calculous and two, acalculous. All calculous carcinomas showed grossly superficial type and two acalculous, polypoid type. All of them were histologically differentiated adenocarcinoma. In advanced carcinoma, thirty-one of 42 cases were calculous and eleven, acalculous. Fifteen of 31 calculous cases showed polypoid type and sixteen, diffuse infiltrative type. In all cases with the latter type, the cystic duct was completely obstructed by impacted gallstones. All calculous carcinomas were histologically belonged to differentiated adenocarcinoma, mucinous carcinoma or adenosquamous carcinoma. On the other hand, six of 11 acalculous carcinomas revealed grossly polypoid type, and histologically differentiated adenocarcinoma or mucinous carcinoma. The remaining five were of diffuse infiltrative type, and of poorly-differentiated adenocarcinoma or signet-ring cell carcinoma. From these data, it may be highly suggestive that differentiated adenocarcinoma or adenosquamous carcinoma of histologic type irrespective of it's gross type is characteristic of calculous carcinoma, and poorly-differentiated adenocarcinoma or signet-ring cell carcinoma possessing primary diffuse infiltrative growth, of acalculous. The majority of the superficial type in early carcinoma and the diffuse infiltrative type in advanced are considered as a secondary modified gross appearance by direct or indirect effects of coexistent gallstones onto the main tumor.

Adenocarcinoma↗
PubMed1985

[Controversy on early carcinoma of the gallbladder based on the results of surgical treatment].

Seventy-two cases of carcinoma of the gallbladder removed surgically were retrospectively studied to evaluate the relationship between stage and prognosis, and to define early carcinoma of the gallbladder. These cases were staged according to general rules for surgical studies on cancer of biliary tract (Japanese Biliary Surgical Society). Stage I patients were 23, composed of 13 of mucosal carcinoma, 7 with involvement of the muscularis and 3 with microinvasion into the subserosa. Stage II and III were 14, and Stage IV 35. All patients with mucosal carcinoma were alive with the exception of the one case who died of other cause. Two cases of the remaining 10 Stage I died of recurrence of carcinoma 1.5 years and 2 years after surgery. Five-year survival rates were 100 percent in cases with mucosal carcinoma, 75 percent in cases with muscular involvement or subserosal microinvasion, 31.6 percent in Stage II and III cases. Stage IV was fatal and most of them died within 6 months after surgery. From our data, it may be rational that the early carcinoma of the gallbladder is defined as the carcinoma of which invasion is limited to the mucosa.

Adult↗
PubMed1984

Carcinoma with lymphoid stroma of the gallbladder--a case report.

A case of carcinoma with lymphoid stroma of the gallbladder is presented. Carcinoma of this type in the breast or the stomach is specified under the designation of carcinoma with lymphoid stroma or infiltration because of a relatively favorable prognosis. The characteristic morphological features are described and the significance of prognostic favorability is discussed together a short review of the literature. This seems to be the first documentation of such a case.

Aged↗
PubMed1984

[Monitoring of the preovulatory follicles--a comparison of ultrasound and laparoscopy].

Accurate prediction of the time of ovulation is important for infertile women considering in vitro fertilization. Preovulatory follicular growth was measured by using ultrasound every 2 to 24 hours and laparoscopic aspiration of the follicular fluid was performed in sixty six patients with clomiphene-HCG cycle. The mature oocyte was examined in aspirated fluid. In ten fertile women with spontaneous cycles, preovulatory follicles were measured by ultrasound and the largest follicular diameter was 18.7 +/- 1.9mm (mean +/- S.D.), of which the growth rate was 1.39mm/day. Follicular diameter was well correlated with the aspirated volume (r = 0.847; p less than 0.001), the largest diameter of the dominant follicle being 28.3 +/- 1.5mm and the second follicle 23.3 +/- 6.1mm. The second follicle grew rapidly after HCG injection and the mature oocyte was collected in spite of rupture of the dominant follicle. The size of the dominant follicle reaching a maximum diameter of 25mm remained almost unchanged for 24 hours before ovulation. The recovery rate of mature oocyte by ultrasound in monitoring of follicles was 87.5%. Ultrasound is a useful technique to use in monitoring follicular growth and ovulation.

Adult↗
PubMed1984

Composite tumor (ordinary adenocarcinoma, typical carcinoid, and goblet cell adenocarcinoid) of the gallbladder: a variety of composite tumor.

This case may represent the first reported of a composite tumor with three neoplastic components in the literature. The tumor was composed of discrete areas of an ordinary adenocarcinoma, a typical carcinoid tumor, and a goblet cell adenocarcinoid. The unique case in the gallbladder is reported and the histogenesis of this rare tumor is discussed with a brief review of the literature.

Adenocarcinoma↗
PubMed1981

Prevention of gastric ulcers in swine by feeding of sodium polyacrylate.

Five experiments involving 169 swine were conducted to determine the effects of 0.1% to 0.5% sodium polyacrylate (PANa) in rations of growing swine on weight gain, feed conversion, and occurrence of esophagogastric lesions. Seemingly, PANa prevented swine from developing esophagogastric ulcers. Esophagogastric ulcers or erosions occurred in 42.9% of the swine fed a PANa-free diet, but occurred in only 9.8% of those fed a diet containing PANa. Addition of PANa (0.1% to 0.5%) to the growing ration increased weight gain and feed conversion. The anti-ulcer effect of PANa was discussed.

Acrylic Resins↗