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Biomedical subjects

M Uehara

Publications and source records attributed to M Uehara.

At least 145 records · Page 8Linked to original sources
PubMed1993

Mitosis of mast cells in skin lesions of atopic dermatitis.

Mitoses of mast cells with cytoplasmic granules were observed in 6 out of 55 skin lesions of atopic dermatitis. It is thus likely that the local proliferation process of mast cells contributes to the increased number of mast cells in skin lesions of atopic dermatitis. Mitotic mast cells with cytoplasmic granules were also observed in 2 out of 8 dinitrochlorobenzene contact dermatitis lesions provoked in patients with atopic dermatitis. It is suggested that some mast cells in skin lesions of atopic dermatitis might be involved in the pathogenesis of type IV eczematous skin lesions of the disease.

Adolescent↗
PubMed1993

Descendant family history of atopic dermatitis.

Descendant family history of atopic dermatitis was examined in 270 adult patients with this skin disease and their 529 children. Of the 529 children, 316 (60%) had a history of atopic dermatitis. Boys and girls were equally affected. The prevalence of affected children was 56% (180/321) in those patients whose spouses did not have a history of both atopic dermatitis and respiratory atopy, 81% (48/59) in those patients whose spouses had a history of atopic dermatitis, and 59% (88/149) in those patients whose spouses had a history of respiratory atopy only. These results indicate that the mode of inheritance of atopic dermatitis is autosomal dominant. They also suggest that the genetic background of atopic dermatitis is not identical with that of respiratory atopy.

Adult↗
PubMed1993

New insight into oncoprotein-targeted antitumor effect: herbimycin A as an antagonist of protein tyrosine kinase against Ph1-positive leukemia cells.

Herbimycin A, a benzoquinonoid anasamycin antibiotic, has been shown to reserve the oncogenic phenotypes of p60v-src transformed cells by the virtue of the inhibition of src protein tyrosine kinase. Furthermore, we previously demonstrated that herbimycin A displayed the antitumor activity on Ph1-positive leukemia cells and bcr/abl oncoprotein-associated transformed murine hematopoietic cells with the transfection of a retroviral vector expressing bcr/abl. Herbimycin A showed preferential inhibition on the in vitro growth of Ph1-positive leukemia cells and bcr/abl oncoprotein-associated murine hematopoietic cells through the inhibition of bcr/abl tyrosine kinase activity and the reduction of subsequent phosphotyrosyl proteins. Recently, from the view of investigating the oncogenic significance or of developing a future clinical application in malignancies, several developing agents targeted against oncoprotein have been tried. We reviewed the present progress in the mechanism of oncoprotein-targeted antitumor effects and focused on herbimycin A-induced antitumor activity on Ph1-positive leukemia cells.

Animals↗
PubMed1993

[Influence of epinephrine infusion on the metabolism of exogenous lipid emulsions after 70% hepatectomy in the rat].

We investigated the effects of epinephrine on the metabolism of a lipid emulsion administrated to rats after 70% hepatectomy. Sprague-Dawley rats were underwent 70% hepatectomy and then were given lipid total parenteral nutrition (lipid-TPN) for 96 h. To increase catabolism after hepatectomy, epinephrine (Bosmine) infused continuously with the lipid-TPN. With increasing doses of the epinephrine, weight loss and the increased urinary excretion of nitrogen and 3 methyl histidine were observed. This indicated that administration of epinephrine after hepatectomy produced a model of increased surgical stress. As the epinephrine dose increased the linoleic acid content of the hepatic phospholipids increased and the arachidonic acid decreased at 96 h after hepatectomy. Epinephrine apparently inhibited the activity of the delta 5.6 desaturase involved in the biosynthesis of polyunsaturated fatty acids, leading to changes of the cell membrane fatty acid composition that could possibly influence membrane function. Thus, the fatty acid composition of lipid emulsions for TPN should be tailored according to the severity of surgical stress, because the pattern of secretion of stress hormone is dependent on the degree of such stress.

Animals↗
PubMed1993

[A prospective study on necessary and sufficient retinal photocoagulation for diabetic retinopathy].

The present studies were performed to determine whether slight photocoagulation was better than heavy photocoagulation for the early stage of progressive proliferative diabetic retinopathy (PDR). The authors selected 17 patients who had bilateral PDR of equal severity (BI or BII stage according to Fukuda's classification). In every case, we randomly performed heavy photocoagulation (laser burns were 1142 +/- 179) to one eye and slight photocoagulation (405 +/- 166) to the other eye. More than 6 months after the last photocoagulation, the effects of these treatments were compared in both eyes by fundus pictures, visual acuity and posterior vitreous fluorophotometric values. The results of judgement by fundus pictures and by vitreous fluorophotometric values were in perfect agreement. Eight cases (47%) in whom eyes received slight photocoagulation showed result better than the other eye. Two cases (12%) which received heavy photocoagulation were better than the other eye. Seven cases (41%) showed the same level of severity. No significant differences were found between slight and heavy photocoagulation.

Adult↗
PubMed1992

[Reproductive and developmental toxicity studies of FUT-187. (I)--Fertility study in rats with oral administration of FUT-187].

FUT-187 was given orally at 20, 120 and 720 mg/kg during the pre-pairing period (63 days prior to pairing in males and 14 days prior to pairing in females) and the pairing period to male and female rats and in the early stage of pregnancy (days 0 through 7 of gestation) to female rats, and the effects of the test compound on male and female reproductive performance and fetal development were evaluated. One male of the 720 mg/kg group died due to treatment. Temporary salivation was observed in males and females in the 20 mg/kg or more groups. In males, increases in the weight of the pancreas in the 120 mg/kg or more groups and the adrenals in the 720 mg/kg group, a depression of body weight gain and decreases in food intake and weight of the carcass in the 720 mg/kg group were statistically significant in comparison with controls. In females, an increase in the weight of the pancreas in the 120 mg/kg or more groups, a slight depression of body weight gain during the early stage of pregnancy and a decrease in the food intake, and a decrease in the weight of the carcass in the 720 mg/kg group were statistically significant in comparison with controls. No dose-related changes were found in the estrus, copulation, insemination and fertility indices. In fetuses, decreased numbers of corpora lutea, implantation and live fetuses were observed in the 720 mg/kg group. There were no treatment-related abnormalities in fetal mortality, sex ratio, weights of fetuses and placenta, and external and visceral examinations. Based on these results, it is concluded that the no-effect-dose levels of FUT-187 are less than 20 mg/kg for the parents, 720 mg/kg for reproductive performance and 120 mg/kg for fetal development.

Administration, Oral↗
PubMed1992

[Reproductive and developmental toxicity studies of FUT-187. (V)--Perinatal and postnatal study in rats with oral administration of FUT-187].

FUT-187 was given orally at 20, 120 and 720 mg/kg to female rats during the perinatal and postnatal periods and the effect on dams and offspring were evaluated. One dam during the terminal period of gestation and 3 dams after delivery in the 720 mg/kg group died due to FUT-187. In dams, an increased pancreas weight in the 20 mg/kg or more groups, temporary salivation after dosing in the 120 mg/kg or more groups, and a depression of body weight gain and decreased food intake and weight of the carcass in the 720 mg/kg group were statistically significant in comparison with controls. In offspring, postnatal death rate in the 720 mg/kg group tended to increased. Decreased body weight gain and delayed appearance of abdominal hair and descent of testis in the 720 mg/kg group were statistically significant in comparison with controls. There were no treatment-related abnormalities in visceral examination, organ weight, skeletal examination, sensory function, behavioral function, learning ability or reproductive function. Based on these results, it is concluded that the no-effect-dose levels of FUT-187 are less than 20 mg/kg for dams, and 120 mg/kg for reproductive performance of dams and offspring development.

Abnormalities, Drug-Induced↗
PubMed1992

Familial primary hyperparathyroidism: study of the pedigree in three generations.

The familial occurrence of primary hyperparathyroidism in which the proband is a 55-year-old man is reported. His 58-year-old sister and 40-year-old brother had undergone partial parathyroidectomy, and histological examination revealed hyperplasia in both cases. Their father and a daughter of the proband had a history of nephrolithiasis. The three siblings showed high levels of plasma parathyroid hormone (even the two postoperative cases). All of them had a history of nephrolithiasis and peptic ulcers. In the proband, image studies did not reveal any abnormality in the neck region. At present, the three cases do not exhibit any abnormalities in the pancreas or the pituitary by imaging studies and endocrine tests.

Adult↗
PubMed1992

Type I allergy to foods in atopic dermatitis. Comparison between RAST-positive and RAST-negative cases.

Radioallergosorbent tests (RASTs) with five common foods were performed in 183 patients with atopic dermatitis. The results showed that about half of the patients had type I allergy to at least one of the five foodstuffs. The RAST results correlated roughly to the severity of dermatitis. In each group of patients with mild, moderate and severe atopic dermatitis, positive RAST reactions to common foods occurred predominantly in patients who had a personal or a family history of respiratory atopy. Positive RASTs were rare in patients with pure atopic dermatitis who had neither personal nor family history of respiratory atopy. It seems likely that type I food allergy occurs predominantly in those patients with atopic dermatitis who have a predisposition to respiratory atopy.

Adolescent↗
PubMed1992

Lymphocyte transformation test for house dust mite in atopic dermatitis: relationship between mite antigens for type I and type IV allergy.

Using a crude extract obtained from Dermatophagoides farinae, and its four fractions (I, II, III and IV) partially purified by high-speed gel filtration chromatography, scratch tests and lymphocyte transformation tests (LTTs) were performed on 37 patients with atopic dermatitis. Crude mite antigen provoked positive scratch test reactions in 25 (68%), and positive LTT reactions in 19 (51%) of the 37 patients examined. Mite antigen fractions I, II, III and IV induced a positive LTT reaction in 19, 22, 17 and 7 patients, respectively. Of the 65 positive LTT reactions, 25 accompanied a positive scratch test to the antigen fraction which provoked the positive LTT reaction, but 40 accompanied a negative scratch test. These findings suggest that house dust mite antigen fractions for type IV allergy are different from those for type I allergy in a considerable proportion of patients with atopic dermatitis.

Adolescent↗
PubMed1992

Mast cell invasion of peripheral nerve in skin lesions of atopic dermatitis.

To ascertain whether or not a spatial relationship between mast cells and peripheral nerves is present in skin lesions of atopic dermatitis, 10 biopsy specimens of the skin lesions were examined with both semi-thin and ultrathin serial sections. Mast cell invasion of peripheral nerves was observed in 9 out of the 10 biopsy specimens (4 subacute lesions, 3 lichenified lesions, and 3 prurigo lesions). The mast cells within peripheral nerves often showed degranulation, and the nerve fiber bundle showed conspicuous edema. The degranulation of mast cells within peripheral nerve bundles and edema of the nerve bundle may pay a role in provoking or aggravating itchiness of atopic dermatitis.

Adolescent↗
PubMed1992

Spectroscopic quantitative analysis of blood glucose by Fourier transform infrared spectroscopy with an attenuated total reflection prism.

As an alternative way of long-term glycemic monitoring, the glucose measurement by analyzing Fourier transform infrared absorbance spectra with an attenuated total reflection prism has been developed. In glucose aqueous solution, glucose has characteristic absorptions at the wave numbers of 1033 and 1080 cm-1 and the absorption intensities are proportional to glucose concentrations. In serum and whole blood samples, however, red blood cell corpuscles, serum albumin and serum gamma-globulin interfere with the absorbance spectra of glucose and shift the base line upward significantly. Therefore, to eliminate these interferences in serum and whole blood samples, the feasibility of the calibration curves obtained by using difference absorbance spectra with those of fasting samples was studied. As a result, highly significant correlations between glucose concentrations estimated by Fourier transform infrared spectroscopic method and those measured by glucose oxidase method were obtained (r = 0.981 and 0.989 for serum and whole blood samples, respectively). From these experiments, it was concluded that by infrared spectroscopy glucose concentrations in the serum and whole blood samples could be measured quantitatively or monitored if the base line drifts and interferences were subtracted.

Blood Glucose↗
PubMed1991

Abnormal glucagon response to arginine and its normalization in obese hyperinsulinaemic patients with glucose intolerance: importance of insulin action on pancreatic alpha cells.

An excessive glucagon secretion to intravenous arginine infusion was found in obese hyperinsulinaemic patients with glucose intolerance. This study was designed to determine whether the glucagon hyperresponsiveness to arginine in these patients would improve by insulin infused at a high enough dose to overcome insulin resistance. By infusing high dose insulin during arginine infusion, the previously exaggerated glucagon response to arginine could be normalized. To normalize the abnormal glucagon response, insulin doses of 4.2 +/- 0.7 and 3.8 +/- 0.5 IU were required during arginine infusion in obese hyperinsulinaemic patients with impaired glucose tolerance and Type 2 (non-insulin-dependent) diabetes mellitus, respectively. This achieved plasma peak insulin levels 3 to 4 times higher than those observed in non-obese healthy subjects. Furthermore, we clarified whether or not the effect of normalizing insulin action and/or glycaemic excursions contributed to normalizing the exaggerated glucagon response to arginine in these patients. Blood glucose was clamped while high dose insulin was infused at the same levels as observed during the arginine infusion test with no insulin infusion. As a result, normalization of the exaggerated plasma glucagon response was achieved, whether hyperglycaemia existed or not. These results clearly demonstrate that, similar to non-obese hypoinsulinaemic Type 1 (insulin-dependent) and Type 2 (non-insulin-dependent) diabetic patients, the exaggerated Alpha-cell response to arginine infusion in obese hyperinsulinaemic patients with glucose intolerance is secondary to the reduction of insulin action on the pancreatic Alpha cell, and that the expression of insulin action plays an important part in normalizing these abnormalities.

Adult↗
PubMed1991

A changing pattern of causes of death among diabetic during a 25-year period in the Osaka District, Japan.

A total of 23,577 death certificates mentioning diabetes, in 10,927 cases as the underlying cause and in 12,650 cases as a contributory condition, were selected from approximately 1,000,000 death certificates filed in Osaka Prefecture, Japan, during a 25-year period between 1960 and 1984. These certificates were analyzed by use of a multiple coding system. The proportion of deaths associated with diabetes, which was only 0.88% of the total deaths in the period 1960-1964, increased to 3.49% in the period 1980-1984. It was also notable that the proportion of certificates with diabetes as the underlying cause of death to the total number of certificates mentioning diabetes decreased strikingly, from 60.8% in 1965-1969 to 36.8% in 1980-1984. Among diabetic subjects, the proportion of patients aged 65 years or over at death increased markedly during the same period, from 47.2% to 68.8%, and a clear increase in the mean age at death was apparent during the 25-year period. Remarkable changes in the underlying causes of death among diabetics were observed during the study period; an increase in disease of the circulatory system, and especially in disease of heart was noted. In the period 1980-1984, disease of heart exceeded cerebrovascular disease which had been the most frequent cause of death in diabetic subjects until that period. An increase in renal disease, including diabetic nephropathy, which was not observed in the standard analysis of underlying causes, was confirmed in the analysis of contributory conditions which was based on the multiple coding system.

Adult↗
PubMed1991

Successful treatment of disseminated cutaneous cytomegalic inclusion disease associated with Hodgkin's disease.

A case of disseminated cutaneous cytomegalic inclusion disease associated with Hodgkin's disease is described. The patient had a diffuse eruption of pruritic, erosive erythematous nodules. Histologically, many inclusion bodies were observed in perivascular areas of the skin lesions. Immunohistologically, the inclusion bodies positively stained with both anticytomegalovirus antibody and anti-factor VIII-related antibody. On electron microscopy many virus particles and dense bodies were found in the area where inclusion bodies were observed. Treatment with high-dose intravenous acyclovir and a large amount of immunoglobulin resulted in prompt healing of the skin lesions. Subsequently, the patient's Hodgkin's disease was well controlled on chemotherapy. The patient steadily improved without relapse of skin lesions 1 year after antiviral therapy was administered.

Aged↗
PubMed1991

[Clinico-pathological findings of recurrent pleomorphic adenomas of the parotid gland].

We analyzed the clinical courses and pathological findings of six recurrent benign pleomorphic adenomas of the parotid gland, compared with fifty-nine primary pleomorphic tumors. Primary cases were treated with extirpation of a tumor nodule with a surrounding area of parotid tissue with preservation of the facial nerve. Of the 59 patients, 42 who had follow-up information have had no recurrent tumors during a average 6.6 years follow up period. On the other hand, all six recurrent cases had received surgical enucleation or excisions elsewhere. To avoid the recurrence of the primary pleomorphic adenoma it was considered to be very important to remove a tumor completely without damaging the tumor capsule. CT scan is very useful to know the relationship between the recurrent tumor and the surrounding important organs. In order to determine the surgical approach and procedures for those cases, it is important to obtain the information of the previous pathological diagnosis or surgical procedures. For the recurrent tumor, complete extirpation of the tumor and surrounding operative scar was necessary for the management of the tumor. When the tumor was tightly adhesive with the facial nerve, sacrifice of the nerve and immediate nerve transplantation might be required.

Adenoma, Pleomorphic↗