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Biomedical subjects

M Ulmer

Publications and source records attributed to M Ulmer.

27 records · Page 2Linked to original sources
PubMed1984

Influence of diet composition on obesity, hyperlipemia and liver steatosis in Zucker fa/fa rats pair-fed with Zucker Fa/- rats.

The effect of a four-week experiment on ten fa/fa Zucker rats (aged seven weeks at the beginning) fed on a lipid-rich diet (HL: 31 per cent w/w lipids, 45.6 per cent starch) was compared to that of a control diet (C: 10 per cent lipids, 66 per cent starch) on control Fa/- rats using a special pair-feeding apparatus that made it possible to obtain an identical intake rhythm. Energy level of the intake was significantly higher for the HL diet than for the C diet. At the end of the experiment, fa/fa rats remained obese and hyperlipemic, and still showed liver steatosis. With equal energy levels ingested, the obesity of fa/fa rats was comparable for both diets; hypertriglyceridemia and hypercholesterolemia were identical for both diets. When compared to the C diet, the HL diet modified neither their obesity nor their hyperlipemia. Obese rat liver on the HL diet showed lower levels for triacylglycerols, cholesterol, GGT, ALT, LDH and aldolase activities, while hepatic glycerol kinase and AST activities were higher than and comparable to, respectively, the C diet. Thus the HL diet led to a decreased liver steatosis for fa/fa rats as compared to the C diet.

Animals↗
PubMed1981

[Comparison in genetically hyperlipoproteinemic and normal rats of the captation and incorporation of isotopic oleic acid and glycerol at high concentration by the perfused liver].

Perfusions of isolated livers from genetically hyperlipoproteinemic Zucker fa/fa and normolipemic Zucker Fa/- rats are performed with loads of ]9,10-3H2] oleic acid and [1-14C] glycerol. The hepatic acylglycerols anabolism from these precursors is higher in the fa/fa rat than in the control Fa/- rats. Synthesis by esterification (of oleic acid) is more increased than de novo synthesis (from glycerol). The increase in lipid anabolism is due to an augmentation of the hepatic cellular mass, but this anabolism is not regulated in the same way than in the normal rat.

Animals↗
PubMed1981

[Comparison in genetically hyperlipoproteinemic and normal rats of the secretion of lipids synthesized by isolated livers perfused with isotopic oleic acid and glycerol at high concentrations].

After perfusing isolated livers of Zucker fa/fa ad Fa/- rats with loads of [9,10-3H2] oleic acid (346 mumol) and [1-14C] glycerol (115 mumol), glycerol inhibited the hepatic secretion of triacylglycerol and phospholipids in the two groups of rats. However, the amount of acylglycerols synthetized from these exogenous substrates is slightly higher in the obese rats than in the normal rats. These results suggest that glycerol, present in high amounts in blood of fa/fa rats, failed to regulate triacylglycerols and phospholipids secretion.

Animals↗
PubMed1981

[Incorporation of odd-numbered fatty acids into the hepatic lipids of Wistar rats].

Isolated Wistar rat livers are perfused with a recirculating medium which contain even- and odd- numbered fatty acids provided by yeast Candida tropicalis grown on n-paraffins. After perfusions odd- numbered fatty acids (OFA) (15:0, 17:0, 17:1) are mainly present in hepatic triacylglycerols, the latter are secreted in the circulating medium. Then OFA can participate in lipidic metabolism as even- numbered fatty acids.

Animals↗
PubMed1980

[Comparison of triglyceride and phospholipid synthesis in isolated Wistar rat liver perfused with high quantities of oleic acid and glycerol].

In a recirculating system, [9,10(-3)H2] oleic acid (346 mumol) and [1-14C] glycerol (115 mumol) are perfused into livers of 18-h fasting Wistar rats. These precursors are incorporated in same amounts into triacylglycerols, and in amounts growing up with the duration of the experiment (5 to 120 min). Their incorporation is slight into phospholipids. However, during the experiment, the increase of 3H/14C ratio of every acylglycerols shows that more lipids are synthetized in the acylation way than in the de novo way. The only synthesis of phospholipids, studied in the two ways, seems to be regulated, unlike the one of triacylglycerols in those experimental conditions.

Acylation↗
PubMed1980

[Secretion into the circulating medium of lipids synthetized by isolated Wistar rat liver perfused with high concentrations of oleic acid and glycerol].

In previously 18-h fasting Wistar rats, the liver is isolated and perfused with [9, 10(-3)H2] oleic acid (346 mumol and [1-14C] glycerol (115 mumol). Then, in a circulating medium, the secretion of triacylglycerols -- synthetized de novo and by esterification of exogenous oleic acid -- and VLDL is inhibited. On the other hand, the secretion of phospholipids is getting away that regulatory process.

Animals↗
PubMedDate not supplied

Influence of caloric intake on gastric inhibitory polypeptide, VIP and gastrin release in man.

Blood glucose, gastric inhibitory polypeptide (GIP), vasoactive intestinal polypeptide (VIP) and gastrin secretions were measured over a three-hour period following the ingestion by normal subjects of a mixed meal with two different caloric levels (1055 Kcal and 1192 Kcal). No VIP secretion was observed after either meal. Gastrin release was not modified by the increase of caloric intake (mainly carbohydrates and lipids), whereas GIP secretion was significantly more important after the meal with the highest caloric value (peak at 30 mm: 499.5 +/- 250.4 vs. 273.4 +/- 128.7 pg/ml and integrated response 53.3 +/- 20.5 vs. 28.2 +/- 9.9 ng X ml-1 X 180 min-1-p less than 0.05). This difference could not be attributed to glucose since the blood glucose levels were not significantly different. It is more probably related to the total amount of ingested food. This suggests the existence of rapid mechanisms of adaptation to the incoming load of the GIP-producing cells.

Adult↗
PubMedDate not supplied

Evaluation of the VIDAS Listeria (LIS) immunoassay for the detection of Listeria in foods using demi-Fraser and Fraser enrichment broths, as modification of AOAC Official Method 999.06 (AOAC Official Method 2004.06).

A multilaboratory study was conducted to compare the VIDAS LIS immunoassay with the standard cultural methods for the detection of Listeria in foods using an enrichment modification of AOAC Official Method 999.06. The modified enrichment protocol was implemented to harmonize the VIDAS LIS assay with the VIDAS LMO2 assay. Five food types--brie cheese, vanilla ice cream, frozen green beans, frozen raw tilapia fish, and cooked roast beef--at 3 inoculation levels, were analyzed by each method. A total of 15 laboratories representing government and industry participated. In this study, 1206 test portions were tested, of which 1170 were used in the statistical analysis. There were 433 positive by the VIDAS LIS assay and 396 positive by the standard culture methods. A Chi-square analysis of each of the 5 food types, at the 3 inoculation levels tested, was performed. The resulting average Chi square analysis, 0.42, indicated that, overall, there are no statistical differences between the VIDAS LIS assay and the standard methods at the 5% level of significance.

Animals↗