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M Unoura

Publications and source records attributed to M Unoura.

At least 91 records · Page 5Linked to original sources
PubMed1989

[Clinicopathological study of juvenile hepatocellular carcinoma].

Clinicopathological analyses were performed on 11 cases of juvenile (less than 40 years) hepatocellular carcinomas (HCC) and compared with those of 187 cases of nonjuvenile HCCs. 91% of juvenile HCC cases were positive for HBsAg in their sera and it was much higher than that of nonjuvenile group (p less than 0.05). Familial clustering of HBV carriers or advanced liver diseases was found in 50%. 73% of juvenile HCC cases had cirrhosis. Abdominal pain was found most frequently as initial symptom. All except one case, who had surgical resection of the tumor, were rapidly fatal. Interestingly the association of paraneoplastic syndrome (PNS) was more frequently seen in juvenile HCC cases (36.3%) than in nonjuvenile HCC ones (5.9%, p less than 0.05). In juvenile HCC cases with PNS, LC was less associated, serum alphafetoprotein tested higher and prognosis was worse than those without PNS. Therefore, these results imply that HBV plays an important role for the development of juvenile HCC and juvenile HCC patients with PNS show characteristic features in HCC patients in Japan.

Adult↗
PubMed1988

An autopsy case of primary sclerosing cholangitis with sequential histologic observations of the liver.

A rare autopsy case of primary sclerosing cholangitis with sequential histologic observations of the liver is described. The patient, a 62-year-old female at the time of autopsy, presented with prolonged cholestasis of about 9 years duration. Initial (at 53 years) and second (at 59 years) liver biopsies disclosed fibrous enlargement of the portal tracts with loss of interlobular bile ducts, lymphoplasmacytic infiltration, a few epithelioid granulomas, piecemeal necrosis, atypical ductular proliferation and deposition of copper granules. Hypergammaglobulinemia with elevated IgM was also noted. These clinicopathological features resembled primary biliary cirrhosis. However, no florid duct lesions were found, and absence of antimitochondrial antibodies and cholangiographic demonstration of a beaded biliary tree favored a diagnosis of primary sclerosing cholangitis. The autopsied liver disclosed sclerosis and cholangioectases of the intra- and extrahepatic biliary tree in addition to biliary cirrhosis. The histology of the biliary tree disclosed nonspecific fibrosing inflammation in the extra- and intrahepatic biliary tree. Other autopsy findings included chronic thyroiditis, sialoadenitis and pancreatitis.

Autoimmune Diseases↗
PubMed1988

Pathologic study of primary biliary cirrhosis of early histologic stages presenting cholestatic jaundice.

While cholestatic jaundice usually develops in the advanced stages of primary biliary cirrhosis, our series of this disease disclosed that 8 of the 88 cases presented cholestatic jaundice in the early histologic stages (stages 1 and 2). These patients frequently presented esophageal varices (57%) and showed a low incidence of positive mitochondrial antibodies compared to the non-jaundices cases in the same histologic stages. Histologic changes of livers from the jaundiced patients failed to show fundamental differences from those of the non-jaundiced patients, except for the presence of bile plugs and extensive bile duct loss in the former. Deposition of orcein-positive granules and increased hepatic copper content, suggesting prolonged cholestasis, were rather advanced, and inflammatory changes in the portal tracts and piecemeal necrosis were rather mild in the jaundiced patients. These data suggest that cholestatic jaundice occurs in a few patients in the early histologic stages on a biopsy with extensive bile duct loss and features of prolonged cholestasis; it is not clear whether such patients are a small separate cluster in PBC, or not.

Adult↗
PubMed1987

Analysis of the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10).

The normal woodchuck karyotype was determined by the G-banding technique, and the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10) were analyzed and compared with it. The normal woodchuck karyotype was 38,XY in a male, and 38,XX in a female. Autosomes were classified into 2 groups (A and B). Group A consisted of 13 pairs (1-13) of metacentric or sub-metacentric chromosomes, and group B consisted of 5 pairs (14-18) of telocentric or sub-telocentric ones. The X chromosome was sub-metacentric, and the Y chromosome was telocentric. In WH257GE10, the modal number was 36. Deletion of one of the chromosome 5 pair, of the chromosome 13 pair, and of the Y chromosome was observed. Two marker chromosomes (M1 and M2) were found. These markers showed the same G-banded patterns except for a small chromosome fragment at the distal part of the long arm of M1. The chromosome aberrations were very stable through successive passages in vitro. These findings suggest that the chromosomal abnormalities found in WH257GE10 might be associated with the pathogenesis of woodchuck hepatocellular carcinoma.

Animals↗
PubMed1986

Human chorionic gonadotropin in primary liver carcinoma in adults. An immunohistochemical study.

Production of human chorionic gonadotropin (hCG) by extragonadal tumours is not a rare phenomenon. In the liver, similar results have been reported in hepatoblastomas. The present study was attempted to survey hCG level in serum and hCG-immunoreactivity in primary liver carcinoma in adults. Although hCG was elevated in serum in 2 (22.2%) of 9 autopsied cases with hepatocellular carcinoma (HCC), the hCG-reactivity of carcinoma cells was found in 2 (2.1%) of 95 HCC cases. Carcinoma cells positive for immunoreactive hCG was found in 2 (15.4%) of 13 cases with cholangiocarcinoma (CC). The patients with hCG-immunoreactivity in carcinoma and/or elevated serum level of hCG failed to reveal distinct clinical and endocrinological disturbance due to excess hCG. The hCG-positive cells were focal within the carcinoma and showed poor histological differentiation in both HCC and CC, and there were no trophoblastic cells. It is suggested that hCG is one of the hormones produced by primary liver carcinoma in adults and can be localised immunohistochemically in a small number of poorly differentiated carcinoma cells.

Adenocarcinoma↗
PubMed1986

Effect of exogenous mouse interferon on murine fulminant hepatitis induced by mouse hepatitis virus type 2.

We investigated the effect of exogenous mouse alpha- + beta-interferon produced by mouse L cells on the growth of mouse hepatitis virus type 2 (MHV-2) in the liver, the development of liver cell necrosis, and survival in murine fulminant hepatitis induced by MHV-2. Murine fulminant hepatitis was induced in 4-week-old male ICR mice by intraperitoneal inoculation of MHV-2. Mouse interferon (10(3) IU/mouse/day) was intraperitoneally injected every day. Exogenous mouse interferon suppressed both the growth of MHV-2 in the liver tissue and development of liver cell necrosis, and prolonged the survival. It was also found that the earlier mouse interferon was administered, the greater was the prolongation of survival.

Animals↗
PubMed1986

Clinical trial of hepatitis B vaccine in the families of HBV carriers.

A hepatitis B (HB) vaccine was given to 75 subjects who were hospital workers and families of HBeAG positive carriers, and the anti-HBs response to the vaccination was studied. Subjects younger than 10 years of age were given 10 micrograms of vaccine and those 10 years or older, 20 micrograms. The second and third injections were given 4 and 24 weeks after the first. Twenty-eight weeks after the first vaccination, anti-HBs was detected in 70 of the 75 subjects (93%). The anti-HBs response was more marked in females and in younger people, but the differences between males and females and between the younger and older groups were not statistically significant. In subjects under 10 years of age, an adequate anti-HBs response was obtained with 10 micrograms of HBsAg protein. There was no clear correlation between the anti-HBs response and the HLA haplotype, because even the siblings who showed the same low anti-HBs response did not have the same HLA haplotype in common, and a difference was observed in the anti-HBs response even among siblings who had the same two HLA haplotypes.

Adolescent↗
PubMed1986

Stable integration of woodchuck hepatitis virus DNA in transplanted tumors and established tissue culture cells derived from a woodchuck primary hepatocellular carcinoma.

The fate of integrated woodchuck hepatitis viral (WHV) DNA was systematically investigated in DNA samples from primary hepatocellular carcinoma (HCC) of woodchucks, solid tumors transplanted in athymic mice derived from a primary HCC of woodchuck, and an established cell line of tissue culture originating from the transplanted tumor. In four of five woodchuck primary HCCs, WHV DNA integration was demonstrated in addition to various amounts of extrachromosomal replicative intermediate WHV DNA. The integration pattern of the primary HCCs does not indicate a common integration site on the host chromosome. The integration pattern in the established cells is identical to that in the transplanted tumor and similar but slightly different from that of the primary HCC. No extrachromosomal or replicative intermediates of WHV DNA were detected in the transplanted tumors or in the established cells of tissue culture. There are three integration sites on the chromosomes of the established cells. Results of Southern hybridization and restriction maps of cloned fragments suggest that all of these integrated WHV DNA sequences are not a complete genome but a part of the genome. A small portion corresponding to the cohesive region of the genome was not detected in all of these integrated WHV DNA. A positive role of WHV DNA integration on the generation of HCC is strongly suggested by the high incidence of WHV DNA integration in woodchuck primary HCCs and the stable maintenance of a certain mode of WHV DNA integration in the hepatoma-derived cell populations during passages of transplantation or serial growth of tissue culture.

Animals↗