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M Urquia

Publications and source records attributed to M Urquia.

At least 19 recordsLinked to original sources
PubMed1996

Denture stomatitis: quantification of interleukin-2 production by mononuclear blood cells cultured with Candida albicans.

Denture stomatitis is usually associated with the presence of yeast, particularly Candida albicans, and several bacteria. In this study mononuclear blood cells were grown in the presence of Candida albicans from a single colony, and interleukin-2 production induced in T lymphocytes was measured. Blood cells were from a population of patients with denture stomatitis and a control group of denture wearers without stomatitis. Induction of interleukin-2 production was correlated with factors that condition denture stomatitis, namely, isolation of Candida albicans in selective medium, age of the denture, and diabetes. Concentrations of interleukin-2 in supernatant and serum were also compared. Significant differences in interleukin-2 production were found between patients with denture stomatitis and controls. Statistical analysis demonstrated a significant association between isolation of Candida albicans and elevated interleukin-2 production in cultures from patients with and without denture stomatitis.

Adult↗
PubMed1993

Detection of anti-HIV antibodies in saliva.

It is sometimes difficult in clinical practice to identify carriers of the AIDS virus. Such identification is of unquestionable value in oral pathology, both for determining the pathogenesis of certain lesions and for assessing their significance to the patient. We evaluated several commercially available diagnostic techniques for the detection of anti-HIV antibodies in serum, and examined the feasibility of adapting such techniques to tests on saliva. The technique chosen for experimental adaptation required only slight modifications for use with this medium. We compared the results obtained in serum from intravenous drug users with a western blot assay designed to detect p24 viral protein, against the findings with a test designed to detect salivary antibodies. The likelihood of cross-reactions in saliva containing high concentrations of other antiviral antibodies was also studied. The specificity and sensitivity of the modified saliva test were 100% and 96% respectively, and no cross-reactions were observed.

AIDS Serodiagnosis↗
PubMed1984

Cardiac echinococcosis and systemic embolism. Report of a case.

We report the case of a 13-year-old boy who, previously in a healthy condition, was admitted to hospital with an acute ischemia of the lower half of the trunk following a slight traumatism. During the surgical operation it was noticed that the embolized material corresponded to hydatid vesicles. The patient died as a result of irreversible anaphylactic shock after the operation. The autopsy revealed the presence of a hydatid cyst in the posterior wall of the left atrium which had ruptured into the atrial cavity. The immediate exeresis of the cardiac cyst is suggested, when it is noticed that the embolized material is made up of hydatid vesicles located in the left cavities of the heart.

Adolescent↗
PubMedDate not supplied

[Clinical performance of the basophil degranulation test. Correlation with other diagnostic technics in allergy (II)].

Although the clinical history is the fundamental method for the diagnosis of reaginic diseases, the use of complementary techniques that gears on the detection of the humoral and cellular effector cells intervening in such affectation is necessary. Thus, we can detect the existence of specific IgE. It can be joined to the basophil membrane by means of techniques "in vivo" as in cutaneous tests, or by means of techniques "in vitro" through cellular techniques in which we apply a biochemical pattern (release of histamine) or a morphological one (HBDT). It can also be free in serum by means of the P-K test or "in vitro" by means of techniques of double antibody. We can also determine, by this last technique, the quantity of the total IgE in serum. In the present work, we review the human basophil degranulation test (HBDT) and its correlation with the prick test, the peripheral blood eosinophilia, the total serum IgE quantification by enzymatic technique and the specific IgE detection by isotope method. In our technique the test was considered positive when the degranulation index with known allergen concentration was greater than 30% for pollens and greater than 25% for D. pteronyssinus with a p less than 0.001. As reported in a previous work, we explain that the normal limits are slightly inferior to those used by some other authors, because in our series, the degranulation average of healthy controls is inferior to that reported by those authors which makes us consider the convenience that the normal limits of degranulation index should be established in a particular way by each technique and allergen.(ABSTRACT TRUNCATED AT 250 WORDS)

Basophils↗