Biomedical subjects

M V Feshchenko

Publications and source records attributed to M V Feshchenko.

4 recordsLinked to original sources

PubMed2006

[An oligonucleotide microarray for detection and discrimination of orthopoxviruses based on oligonucleotide sequences of two viral genes].

An oligonucleotide microarray for detection and identification of orthopoxviruses was developed. Genus specific and orthopoxvirus species-specific regions of the genes encoding chemokine binding and alpha/beta-interferon binding proteins were used as a target. The developed microarray allows the variola, monkeypox, cowpox, vaccinia, camel-pox and ectromelia (mousepox) viruses to be distinguished with a high degree of reliability.

Gene Amplification↗

PubMed2004

[Phylogenetic analysis of chemokine-binding protein gene from orthopoxviruses].

Poxviruses, belonging to the genus of Orthopoxviruses and Leporipoxviruses, express a protein that binds a wide spectrum of CC-chenokines. The above viral protein (vCCI) has no homology by the amino acid sequence with the known cellular receptors of chemokines or with any other proteins represented in databases. Sixty-nine nucleotide sequences of the vCCI protein gene of 4 Orthopoxviruses, which are pathogenic to man, and of 2 species, to whom man is immune, as well as 16 of such sequences borrowed from GenBank were subjected to the phylogenetic analysis. Strains of Orthopoxviruses, except for cowpox viruses (CPXV), were found to cluster together in groups according to their specific attribute. CPXV isolates can be conditionally divided into 2 subgroups, which are much remote from one another evolutionally. Four subgroups were defined for smallpox virus with their strains originating from different geographic regions.

Base Sequence↗

PubMedDate not supplied

[A rapid and effective solid-phase synthesis of DNA-sequence binding polyamides].

A rapid and effective variant of solid-phase synthesis of DNA-sequence-specific polyamides on the basis of 4-amino-1-methylpyrrole-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, beta-alanine, and gamma-aminobutyric acid was suggested. It is based on the use of di- and trimeric oligocarboxamide building blocks, which help reduce the time of synthesis, increase its yield and purity of products, and efficiently use manual synthesis for the synthesis of long oligocarboxamides. The yields of hairpin ligands with up to 10 units are 35-50% and the synthesis takes no more than 6 h.

Alanine↗

PubMedDate not supplied

[Liquid phase method of synthesizing polyamides binding DNA using oligocarboxamide blocks].

A method was developed for the synthesis of sequence-specific polyamides on the basis of 4-amino-1-methylpyrrole-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, beta-alanine, and gamma-aminobutyric acid. Dimeric and trimeric oligocarboxamides were used as building blocks. Our synthetic scheme was applied for the synthesis of DNA minor groove binders containing up to twelve carboxamide units. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.

Amides↗