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Biomedical subjects

M V Garcia

Publications and source records attributed to M V Garcia.

10 recordsLinked to original sources
PubMed1997

[Educational program in universal precaution measures: a methodological approach].

An updating program on measures of universal precautions (M.U.P.) was developed at the Center of Whole Care of Woman's Health (Centro de Atenção Integral à Saúde da Mulher-CAISM). These measures and the procedures in the case of work accident were published in a booklet. First, servants should be aware of the matter of stress and its influence on the quality of life. Then, updating was carried through encouraging the reflection on the consequences of the non-adoption of M.U.P. The answers to 286 pre-tests and 242 post-tests were analyzed and the results showed a significantly higher index of correct answers (p < 0.01), mainly regarding the appropriate use of glove.

Education, Nursing↗
PubMed1993

Changes in mitochondrial rhodamine-123-fluorescence populations of rat hepatocytes in primary culture.

Flow cytometry analysis of mitochondria isolated from rat hepatocytes at different stages of development revealed two different rhodamine-123-stained fluorescence populations distinguishable by their main fluorescence channel. The high-fluorescence population (HFP) was minor, accounting for about 32, 33, and 23% of the total mitochondrial fraction in hepatocytes from preterm, term, and early (1 h) newborn rats, respectively. The percentage of HFP decreased during the first 20 h of hepatocytes in culture from preterm and term fetuses but not those from early newborns, in which the decrease occurred during the second day of culture. However, after 20 h in culture more than 80% of hepatocytes reached the G0/G1 phase, whatever the stage of development in which they were sown. This suggests that the observed changes in the fluorescence populations are not associated with the cell cycle; instead these changes mimic those observed during postnatal development and are therefore presumably due to mitochondrial differentiation.

Animals↗
PubMed1989

[Study of hyperchromatism caused by benzene derivatives].

We report two patients with vitiligo-like lesions caused by contact with compounds containing phenols and toluene. The clinical, histopathologic and ultrastructural findings are compared with vitiligo. Two mechanisms may be present: one from direct enzymatic or cytotoxic action on melanocytes and the other through immunologic reaction.

Benzene Derivatives↗
PubMed1988

Maternal-fetal relationship in ammonia metabolism during late gestation in the rat.

The concentrations of the major metabolites for nitrogen excretion and/or transport in maternal and fetal blood and amniotic fluid during the last 2 days of gestation were investigated. Alanine, glutamine, ammonia and allantoin accumulated in amniotic fluid during late gestation. Urea concentrations increased in amniotic fluid though only during the last day of gestation, suggesting that urea is taken up by the mother through the amniotic membranes. Glutamate did not accumulate in amniotic fluid during late gestation although high concentrations of it were found in fetal blood in the same circumstances, suggesting the occurrence of a mechanism for preventing fetal glutamate disposal.

Alanine↗
PubMed1986

The levels of total protein and protein fractions in the serum of rabbits infected with Eimeria stiedai.

The total protein levels in rabbits infected with Eimeria stiedai together with the alterations in the different protein fractions over a period of 35 days were studied. It was observed that from the second week of infection onwards a progressive increase took place in total proteins; the electrophoretic study revealed that the hyperproteinemia is mainly due to the increase in gamma-globulins accompanied by a decrease in the concentration of albumin and a increase in alpha and beta-globulins. These protein alterations induce an important modification in the albumin/globulin. ratio which decreases considerably.

Animals↗
PubMed1985

Alterations in the activities of subcellular fractions marker enzymes in rat liver and brain by hydrocortisone and corticosterone treatment.

The effect of subcutaneous injection of hydrocortisone and corticosterone on the activity values of some subcellular fractions marker enzymes from rat liver and brain was investigated and compared with controls (without treatment with hormones). The following enzymes were studied (subcellular fraction are shown between parentheses): N-acetyl-beta-D-glucosaminidase and beta-glucuronidase (lysosomes); succinate dehydrogenase = SDH (mitochondria); glucose-6-phosphatase (endoplasmic reticulum); 5'-nucleotidase and Na+-K+-Mg2+ ATPase (plasma membrane). The specific activity of lysosomal enzymes from liver showed no change when rats were injected either with hydrocortisone or corticosterone. The same enzymes from brain showed significant increases in their activities with both hydrocortisone or corticosterone except beta-glucuronidase; this enzyme gave activity values remaining between the control levels, after treatment with corticosterone. The activity of mitochondrial SDH was increased after corticosterone injection either in liver or brain. After hydrocortisone injection, its activity rises significantly in brain (72%), but it falls in liver compared to the control values. Glucose-6-phosphatase behaves similarly in brain or liver fractions; its activity increases always after corticosterone treatment and decreases by hydrocortisone. The plasma membrane marker enzymes did not change practically in brain fractions, excepted Na+-K+-Mg2+ ATPase which tends to rise its activity after hydrocortisone injection. In liver fractions, both 5'-nucleotidase and Na+-K+-Mg2+ ATPase activities increase either by corticosterone or hydrocortisone treatment, except 5'-nucleotidase which specific activity decreases in liver after hydrocortisone treatment.

5'-Nucleotidase↗
PubMed1979

Comparative studies on six blood serum glycosidases from several mammalian species.

1. Peripheral blood serum alpha-D-galactosidase, beta-D-galactosidase, beta-D-glucosidase, alpha-D-mannosidase, beta-D-xylosidase and beta-D-glucuronidase have been studied with a comparative point of view from several mammalian species: Bos taurus L. (bull), Capra hircus L. (goat), Sus scropha var. domestica L. (pig) and man. 2. Fluorimetric and spectrophotometric procedures were used for determination of enzyme activities and pH optima. 3. Glycosidase activity was generally higher with fluorescent substrates than with chromogenic substrates. 4. alpha-D-mannosidase was the most active with both fluorescent and chromogenic substrates. 5. All the studied enzymes had the same pH optimum (4.0) when the chromogenic substrates were used. 6. pH optima of these glycosidases ranged from 3.0 to 5.5 when the fluorescent substrates were used.

Animals↗