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Biomedical subjects

M V Ivanova

Publications and source records attributed to M V Ivanova.

16 recordsLinked to original sources
PubMed1991

[Metabolism of cholesterol esters in cultured macrophages under the action of the calcium antagonists verapamil and nifedipine].

Using mouse macrophage cultures, the effects of verapamil and nifedipine on cholesterol ester metabolism were studied with special reference to the following parameters: i) incorporation of 14C-oleate into cholesterol esters (ChE), ii) contents of common and free cholesterol (FCh), iii) removal of 14C-oleate from ChE and incorporation of 3H-FCh into ChE and, iiii) excretion of 3H-Ch from the cells. Verapamil and nifedipine (10-100 microM) decreased the incorporation of 14C-oleate into ChE, increased the concentration of FCh but had no appreciable effect on the concentration of common Ch in macrophages cultured in the presence of acetylated low density lipoproteins. The drugs stimulated the removal of 14C-oleate from cellular ChE. The pharmacological concentrations (25-75 microM) of verapamil and nifedipine increased the excretion of 3H-Ch from ChE of macrophages in the presence of serum and high density lipoproteins. The results obtained suggest that verapamil and nifedipine mediate the antiatherosclerotic effect via reduction of intracellular synthesis of ChE, stimulation of ChE hydrolysis and cholesterol excretion from the cells.

Animals↗
PubMed1989

[Morphofunctional characteristics of thrombocytes in children with rheumatoid arthritis].

Altogether 205 children suffering from rheumatoid arthritis were examined for the morphofunctional status of platelets. Appreciable changes in the functional properties of platelets were revealed together with ultrastructural disorders. The correlation was noted between the characteristics under study as was their relationship with the disease activity and pattern.

Acid Phosphatase↗
PubMedDate not supplied

[The effect of ketoconazole on the cholesterol content of the blood serum lipoproteins and bile in experimental hypercholesterolemia].

Inclusion of ketonazole (in a daily dose of 400 mg/kg for 8 days) into the diet of intact and cholesterol-fed (5% dietary cholesterol) rats produced a respective 20- and 30-percent lowering of cholesterol content in blood serum. In all the animals, the hypocholesterolemic effect of ketoconazole was realized via a decrease of the concentration of very low and low density lipoproteins. Ketoconazole also gave rise to a reduction of the concentration of cholesterol and bile acids in bile of the intact rats and of cholesterol in bile of the cholesterol-fed animals.

Animals↗
PubMedDate not supplied

[Effect of ketoconazole on the activity of key enzymes of biosynthesis of cholesterol and its esters in the liver of rats maintained on a cholesterol-enriched diet].

Ketoconazole in vivo has been studied for its effect on the activity of key enzymes of the cholesterol and its esters' biosynthesis in the liver and on the cholesterol concentration in certain fractions of blood lipoproteins in normal and cholesterol-fed rats. It is established that ketoconazole decreases cholesterol concentration in low-density lipoproteins and in very low-density lipoproteins as well as decrease the acyl-CoA-cholesterol acyl-transferase activity and increases the 3-hydroxy-3-methyl-glutaryl-CoA-reductase activity in the liver microsomes of intact and test animals. It is supposed that the possible cause of the observed changes can be a disturbance in regulation of basic links of cholesterol metabolism in the liver.

Animals↗
PubMedDate not supplied

[The effect of verapamil and nifedipine on cholesterol esterification and accumulation in macrophages].

Administration of verapamil and nifedipine in doses of 10-75 microM into the culture of mouse peritoneal macrophages incubated in a non-lipid medium with acetylated low density lipoproteins (50 g protein/ml) decreased the incorporation of 14C oleate into cholesterol esters by 50-90%, increased the content of intracellular free cholesterol by 19-79% and did not influence the content of total cholesterol in macrophages. Administration of verapamil and nifedipine in doses of 25 and 50 microM in the culture of macrophages previously enriched with cholesterol reduced the rate of cholesterol esterification by 22-62%. After combined administration of verapamil and nifedipine in doses of 25 microM into the medium the rate of cholesterol esterification in macrophages fell by 72.4%.

Animals↗
PubMedDate not supplied

[The correction of disorders in transcapillary metabolism in children with rheumatoid arthritis by using health resort therapy].

Children with rheumatoid arthritis in remission or minimal activity of the disease were examined after sanatorium treatment. It was established that health resort factors had produced a positive capillarotropic effect in rheumatoid arthritis, i.e. capillarotropic insufficiency, tissue hypoxia, inflammation and proteolysis reduced; microcirculation returned to normal.

Arthritis, Juvenile↗