Biomedical subjects

M V Sukhacheva

Publications and source records attributed to M V Sukhacheva.

4 recordsLinked to original sources

PubMedDate not supplied

[Streptomyces sp. Z-11-6--a new producer of extracellular L-glutamate oxidase].

Glutamate oxidase activity was studied in 1254 Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis were optimized. A multistep-mutagenesis and selection procedure allowed a genetically stable strain, Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate.

Amino Acid Oxidoreductases↗

PubMedDate not supplied

[Extracellular L-glutamate oxidase from Streptomyces sp. Z-11-6: preparation of it and its properties].

Mutagenesis induced with nitrous acid and subsequent selection allowed a genetically stable mutant strain, Streptomyces sp. Z-11-6, to be obtained, whose L-glutamate oxidase activity was 40-fold higher than that of the original natural isolate and was as great as 1.6-1.8 units/ml of culture liquid. A procedure for the isolation and purification of the enzyme was developed; the biochemical properties of the enzyme were studied. Out of 20 amino acids tested (including D-glutamate), the glutamate oxidase from Streptomyces sp. Z-11-6 was active only with L-glutamate. This allows the concentration of L-glutamate to be determined in the presence of other amino acids. Calcium chloride at a concentration of 0.1-0.5% promoted the secretion of the extracellular glutamate oxidase.

Amino Acid Oxidoreductases↗

PubMedDate not supplied

[Photometric method for determining ethanol].

A novel enzymatic photometric assay for ethanol determination using alcohol oxidase and peroxidase is described. The sensitivity of the method allows detecting ethanol in biological fluids (saliva and blood serum). Secondary alcohols and other organic compounds do not interfere with the assay. General-purpose spectrophotometers and photoelectric colorimeters can be used in the measurements. Methanol and propanol can also be determined by this technique.

Alcohol Oxidoreductases↗

PubMedDate not supplied

[Photometric assay for methanol in the presence of ethanol].

A new enzymatic photometric assay for determination of methanol and ethanol in solutions containing both alcohols is described. The assay allows detecting methanol in the concentration range of tens ppm of in the presence of tens per cent of ethanol. The lower determination threshold for methanol is at least 0.002% in the presence of 45% ethanol, with a coefficient of variation of 0.02-0.05. General-purpose spectrophotometers and photoelectric colorimeters can be used in the measurements.

Enzymes↗