PubMed Health⌕ Search

Biomedical subjects

M Vasková

Publications and source records attributed to M Vasková.

6 recordsLinked to original sources
PubMed1995

Light-chain fibroin of Galleria mellonella L.

The posterior section of Galleria mellonella silk glands contains two abundant mRNAs that are identical except for the non-coding tail, which includes either two (1.1 kb mRNA) or three (1.2 kb mRNA) consensus sequences for polyadenylation sites. The transcripts are 40% homologous in the coding as well as non-coding regions with the mRNA encoding light-chain fibroin (L-fibroin) in Bombyx mori; the deduced translation product shows 43% identity with the Bombyx L-fibroin peptide, with all three cysteines conserved. Amino acid analysis of the N-termini of Galleria silk proteins revealed that L-fibroin (25 kDa) occurs in two isoforms, the shorter one lacking the Ala-Pro dipeptide residue at its N-terminus. The 29 and 30 kDa Galleria silk proteins appear to be homologs of Bombyx silk component P25. The results suggest that evolutionary diversification of Galleria and Bombyx L-fibroins involves alternative polyadenylation and proteolytic processing sites.

Alternative Splicing↗
PubMed1989

[Levels of homovanillic acid and 5-hydroxyindoleacetic acid in the cerebrospinal fluid in patients with focal cerebral ischemia].

The investigated group comprised 35 patients where clinical examination revealed focal cerebral ischaemia in the area of the a. cerebri media. In the group of elderly patients above 60 years, as compared with controls, the homovanillic acid (HVA) and 5-hydroxyindolacetic acid (5-HIAA) concentration in cerebrospinal fluid was elevated. In the group of patients under 60 years no changes in the concentration of these metabolites were found. In older patients the increased level of metabolites in cerebrospinal fluid persisted also after three days following the attack, while in younger patients the elevated HVA and 5-HIAA levels were found only in specimens collected up to three days.

Adult↗
PubMed1987

Tn1000 insertional mutagenesis of cloned repressor gene of the phage L: plasmid oligomerization in the presence of F'lac.

An ampicillin resistance plasmid carrying the cloned repressor gene cII of the L phage (Salmonella typhimurium) was conducted by F'lac into an F- recipient. Two types of plasmids were isolated from Apr transconjugants. The majority of plasmids were dimers with one copy of Tn1000 inserted, the minority being monomers with one copy of Tn1000. This proportion remained unaltered when we used the F'lac strain transformed with a monomeric form of the recombinant plasmid as a donor. An extensive oligomerization of pBR322-originating plasmids was proved in the presence of F'lac; its presumable relationship to transposition-related processes is suggested.

Cloning, Molecular↗
PubMed1986

Hot spot for Tn1000 insertions in cloned repressor gene of the L phage.

Insertions of Tn1000 into a cloned fragment of the L-phage genome comprising the repressor gene were prepared. Repressor activities produced by plasmids with insertions were assayed in vivo. As a result, the repressor gene was localized within 0.5 kb near one end of the cloned fragment. Transposon insertions were nonrandomly clustered within the repressor gene and in its close vicinity. An analysis of supercoiled plasmid DNA with the nuclease S1 revealed no distortion of the secondary structure of DNA in this region.

Bacteriophages↗