[Level of serum prealbumin in the diagnosis of liver cirrhosis. First results].
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Biomedical subjects
Publications and source records attributed to M Vernet-Nyssen.
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The electroimmunodiffusion on agarose has been applied for the determination of AFP in human serum. The proposed method can be used for concentrations between 0.6 and 50 mg/l; the reproducibility was judged to be acceptable. For concentrations higher than 10 mg/l, a good correlation was observed between electroimmunodiffusion and radial immunodiffusion.
After recalling the conditions of fixation of iron on human native serum, the authors determined the TIBC on 15 commercial control sera after titration. The transferrin concentration was determined on 13 sera of human origin, by two immunochemical techniques. They were thus able to demonstrate certain sera including a chelator substance other than transferrin, their TIBC depends only on the experimental conditions; such sera are not usable for quality control. Lyophilisation seems to reduce the reactivity of sites of fixation. It is thus necessary to provide a large excess of iron and prolong the duration of contact.