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Biomedical subjects

M Voge

Publications and source records attributed to M Voge.

At least 37 records · Page 2Linked to original sources

Intestinal parasites in Los Angeles, California.

Because of an apparent increase in the number of parasitic infections in Los Angeles, California, a six-month retrospective analysis of data obtained from patient stool examinations for parasitic infections in out-patient populations from two county health facilities in Los Angeles County was compared with a survey published in 1936 and 1960. A significant increase in the incidence of pathogenic intestinal protozoal parasites was found to occur in the areas surveyed. Giardia lamblia and nonpathogenic Endolimax nana occurred with the greatest frequency.

Ascariasis↗

Schistosoma mekongi sp. n. from man and animals, compared with four geographic strains of Schistosoma japonicum.

Schistosoma mekongi sp. n. is described from man and animals in Cambodia. It is compared to 4 geographic strains of Schistosoma japonicum. It differs from S. japonicum in the size of embryonated eggs, in the length of the prepatent peroid in the mammalian host, and in its utilization of a different snail host. The relative usefulness of conventional morphologic criteria in the differentiation of Asian schistosomes is discussed.

Animals↗

Changes in tegumental surface during development of Schistosoma mansoni.

The tegumental surface of immature Schistosoma mansoni was studied with the scanning electron microscope. The surfaces of immature males and females bear no resemblance to that of adult worms and are characterized by having many tegumental folds. The tegumental surfaces of immature males and females are similar, and the dorsal and ventral surfaces of the male are similar before formation of the gynecophoral canal. Transition of the tegumental surface from the juvenile to the adult form begins after worms are in copula and have grown to several millimeters in length.

Animals↗

Immunoglobulins on the surfaces of tetrathyridia of Mesocestoides corti Hoeppli 1925 (Cestoda), from laboratory infections of ICR mice.

7Sgamma2b antibody was detected, by fluorescein labeled antibodies, on the body surfaces and wall of excretory bladder of tetrathyridia of M. corti removed from the peritoneums of 5--24-week laboratory infections of ICR/TIMCO mice. 7Sgamma1, 7Sgamma2a, 7Sgamma3, IgM, IgA, and C3 were not found by use of the same techniques. Tetrathyridia maintained in culture medium to which pooled normal mouse, infected mouse, and horse (control) serums containing all known classes and subclasses of immunoglobulins had been added showed only mouse 7Sgamma2b on their surfaces and wall of the excretory bladder, and then only when incubated in pooled serum from infected mice. No other mouse immunoglobulin classes or subclasses were found attached to the worms, nor was any reaction obtained from tetrathyridia incubated in culture medium alone, normal mouse serum, or horse serum. This suggests a highly selective nonspecific absorption of 7Sgamma2b, or more likely demonstrates the specificity of the antibody for the parasite surfaces and wall of the excretory bladder. The possibility that 7Sgamma2b may be acting in an enhanceing mode is discussed.

Animals↗

Strongyloides fulleborni-like infections in man in Papua New Guinea.

During the course of a survey of intestinal parasites among the inhabitants of villages along the Fly River in the Kiunga region of Papua New Guinea, eggs of a Strongyloides species were found in the feces of several persons. In subsequent studies, 93 (17.8%) of 520 persons examined from five villages were found to be infected with this parasite. The examination of parasitic and free-living stages of the worm revealed that it is very similar to S. fulleborni, a parasite of monkeys, baboons and apes in Africa and Asia, although a definitive identification could not be made with the material available. Since non-human primates have apparently never inhabited New Guinea, the origin of these S. fulleborni-like infections is unknown.

Female↗

Synergistic growth promoting action of L-cysteine and nitrogen upon Hymenolepis diminuta cysticercoids in vitro.

Development in vitro of cysticercoids of the cestode Hymenolepis diminuta in the presence of L-cysteine proceeded twice as rapidly under 100% nitrogen as it did with air as the gas phase. No growth was obtained with the following: D-cysteine, L-cysteine methyl ester, S-aminoethyl cysteine, L-cystine, glutathione (reduced), L-methionine, D-pantothenic acid, taurine, or thioglycolic acid; limited growth only was obtained with ascorbic acid and dithiothreitol. Homocysteine or coenzyme A was as effective as L-cysteine in stimulating the complete development of oncospheres to cysticercoids. Experiments with 14C-L-cysteine indicated that L-cysteine was perhaps incorporated into the organisms.

Animals↗

Axenic growth of oncospheres of Hymenolepis citelli (Cestoda) to fully developed cysticerocoids.

Cysticercoids of the cestode Hymenolepis citelli were grown axenically in vitro from the oncosphere to stages infective for the mammalian host. Reducing agents were an essential component of the culture medium. Of several reducing agents tested, L-cysteine was the most effective. Concentration of reducing agent was critical in that excessive amounts induced abnormal growth.

Amino Acids↗

Axenic development of cysticercoids of Hymenolepis nana.

Cysticercoids of the tapeworm Hymenolepis nana were grown axenically in vitro, from oncospheres hatched in vitro to fully developed organisms infective to mice. A gas phase of 95 N2--5 CO2 was essential for normal development.

Animals↗