Biomedical subjects

M Volgushev

Publications and source records attributed to M Volgushev.

6 recordsLinked to original sources

PubMed1995

All-or-none excitatory postsynaptic potentials in the rat visual cortex.

Intracellular recordings were obtained from supragranular neurons in slices of the rat visual cortex. In approximately 25% of the cells large (0.5-1.6 mV) excitatory postsynaptic potentials (EPSPs) of constant amplitude were observed after minimal, presumably single-fibre stimulation. The amplitude variance of these large EPSPs was surprisingly small and within the range of the variance of the noise. These EPSPs could be reduced in amplitude by paired-pulse and low-frequency stimulation or by raising extracellular Mg2+ concentration. Reduced EPSPs could either continue to behave as all-or-none responses, or they could fluctuate between several amplitude levels. Conversely, responses where the amplitude fluctuated from trial to trial under control conditions could be converted into large all-or-none responses by paired-pulse facilitation. This indicates that the large all-or-none EPSPs were composed of several subunits, probably reflecting the action of several different release sites. It is concluded that these release sites are either independent and operate with a probability close to 1 or, if operating with a lower probability, are coordinated by a mechanism which synchronizes release. Several observations suggest that release probabilities can switch from values close to 1 to 0 with repetitive stimulation or high Mg2+ concentration. Thus, a substantial fraction of single-fibre inputs to supragranular cells possess synapses which operate with high synaptic efficiency and extremely low variance under control conditions but can undergo drastic changes in efficacy when release probabilities are interfered with. Such modifications of release probability could serve as an effective mechanism to regulate the gain of synaptic transmission.

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PubMed1995

Neurophysiological analysis of long-term potentiation in mammalian brain.

Long-term potentiation (LTP) is a persistent increase in postsynaptic response following a high-frequency presynaptic activation. Characteristic LTP features, including input specificity and associativity, make it a popular model to study memory mechanisms. Mechanisms of LTP induction and maintenance are briefly reviewed. Increased intracellular Ca2+ concentration is shown to be critical for LTP induction. This increase is believed to be based on Ca2+ influx secondary to activation of N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Existence of other sources of Ca2+ increase and other critical factors is now becoming evident. They include voltage-dependent Ca2+ channels, Ca2+ intracellular stores, metabotropic glutamate receptors, 'modulatory' transmitters. An example of an involvement of voltage-dependent Ca2+ channels is potentiation induced by intracellular depolarizing pulses. LTP can be divided into decremental earlier (E-LTP) and non-decremental late (L-LTP) phases which explains some inconsistencies in studies of LTP mechanisms. E-LTP is suggested to be based on a transient increase in presynaptic release probabilities. A hypothesis is considered which explains L-LTP by suggesting that Ca2+ activates structural changes leading to an increase in the synaptic gap resistance. This enhances positive synaptic electrical feedback and augments release probability. The hypothesis predicts specific morphological changes, synchronous transmitter release of two or several quanta in some central synapses and the amplification of such synchronization following LTP induction. Data are discussed which maintain these predictions.

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PubMed1994

Induction of LTP and LTD in visual cortex neurones by intracellular tetanization.

Neurones from supragranular layers of rat visual cortex slices were activated by intracellular tetanization (IT) without concomitant presynaptic stimulation. The effect of IT was examined on EPSPs evoked at low stimulation intensity from two subsets of afferents by electrodes positioned in layers II and IV, respectively. In 17 of 23 inputs to 15 cells IT led to changes in EPSP amplitudes which persisted throughout the recording period (from at least 40 min to 3 h). For 10 potentiated inputs (nine cells) and eight depressed inputs (seven cells), EPSP amplitudes measured 30 min after tetanization were 167 +/- 14% and 55 +/- 14% of the pretetanic controls, respectively. In seven cells both inputs changed, in five cases modifications were of the opposite and in two cases of the same polarity.

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PubMed1994

Receptive field analysis and orientation selectivity of postsynaptic potentials of simple cells in cat visual cortex.

Postsynaptic potentials (PSPs) were recorded from cat striate cortical cells by the whole-cell in vivo recording technique using patch-clamp electrodes. EPSPs and IPSPs evoked by flashing bars on the receptive field at different positions and orientations revealed the spatial structure of the excitatory and inhibitory inputs. The elongation of the excitatory input field (length:width ratio) was found to be minimal (mean ratio of 1.7) and much lower than those reported for spike discharges. Two-dimensional receptive field response profiles of early PSPs were recorded by flashing a small spot of light over a square matrix covering the receptive field. These recordings also showed only mild degrees of elongations of the receptive field. Such elongations could be the result of either an excitatory input from the geniculate that is already biased for orientation or an excitatory convergence from a limited number of LGN fields arranged in a row. In most first-order cells, we found that inhibition was contributing significantly to orientation selectivity. Often prominent IPSPs could be evoked by stimuli of nonoptimum orientations. Presence of inhibition could also be inferred by the way that EPSPs were sharply cut off by inhibition. When the amplitude of an EPSP was measured at different latencies after its onset, the EPSP was found to be very broadly tuned to orientation at the beginning, but showing increasing orientation selectivity with time. It is proposed that this progressive development of orientation selectivity is due to (1) inhibitory inputs arriving after the first wave of excitation, (2) intracortical excitatory inputs from other cells tuned to similar orientations, and (3) voltage-sensitive mechanisms such as NMDA channels.

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PubMed1992

Postsynaptic potentials in cat visual cortex: dependence on polarization.

During the investigation of visually evoked postsynaptic potentials (PSPs) of visual cortical neurons, we recorded cell activity under different levels of membrane potential. In some cases, however, dependence of these PSPs on the level of membrane polarization appears to be inconsistent with the conventional scheme. One disagreement was the reduction, instead of an increase, of excitatory potentials during hyperpolarization of the cell. The other point was that depolarization of the cell often leads to increase of the amplitude of both excitatory and inhibitory postsynaptic potentials. This inconsistency may suggest the involvement of voltage-dependent ion channels in generating PSPs to visual stimuli. A possible way of separating the excitatory and inhibitory components of the response by polarization of the cell in spite of the presence of voltage-dependent channels and possible implications of this mechanism in the visual cortex are discussed.

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PubMed1991

Whole cell recording and conductance measurements in cat visual cortex in-vivo.

Long and stable recordings of post-synaptic, action and membrane potentials from visual cortical neurons in-vivo, are possible with the patch-clamp technique. These are comparable to the whole-cell configuration, but with an incomplete seal. EPSPs and IPSPs of normal time course and up to several mV can be recorded. DC potentials ranged from - 30 to - 60 mV and input resistances from 50 to 150 M omega. Injected currents have the same effect as if applied intracellularly. Membrane conductance after electrical stimulation of the lateral geniculate nucleus is increased during the first 20 ms, but decreases from 60 to about 130 ms, during return of the membrane potential to its resting level. The recording method is compared to other intracellular recording techniques in-vivo and in-vitro.

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