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Biomedical subjects

M Vrlík

Publications and source records attributed to M Vrlík.

12 recordsLinked to original sources
PubMed2002

[Importance of determination of lymphocytes in intestinal mucosa biopsy specimens using flow cytometry in the evaluation of ulcerative colitis activity].

In this clinical study, we have examined 10 patients with ulcerative colitis. The study group included 5 men and 5 women. The control group consisted of 6 patients with negative colonoscopic finding performed for differential diagnosis of the dyspeptic syndrome. Patients with ulcerative colitis were divided into two groups according to the clinical pattern, macroscopic endoscopic finding, and histological examination of the bioptic specimens. The Ist group of patients with active stage of ulcerative colitis, the IInd group of patients in remission, without clinical and endoscopic signs of disease activity. We have found that from the followed markers the greatest importance lies in the determination of the standard immunologic profile of the mucosa, the determination of activating markers HLA-DR+, CD69, CD122 and CD71 on T-lymphocytes and CD69 and CD71 on B-lymphocytes. The differences between the control group and the group of patients in active stage of the disease were statistically significant. Statistical significance was also found in some of the above-mentioned parameters when comparing the group of active disease and that one in remission (CD4/CD8 ratio, HLA-DR+, CD122 on T-lymphocytes). Our results imply that the changes in the target tissue contribute to more precise assessment and follow-up of therapeutic effect in non-specific inflammatory bowel disease.

Antigens, CD↗
PubMed1996

[Sepsis. II. Complications and treatment of sepsis].

Sepsis and its complications are serious medical problem of intensive medicine. Therefore recently several therapeutic procedures were tested in sepsis and its complications such as syndrome of multiorgan dysfunction or septic shock. The authors review possibilities how to treat these complications.

Humans↗
PubMed1996

[Sepsis. I. [Definition of the concept and comments on pathophysiology].

During the past five years a marked development occurred as regards knowledge on sepsis and its complications. A new term was introduced-SIRS (systemic inflammatory response syndrome). Two substantial changes pertain to the following facts. First the clinical picture of sepsis need not be due only to infection, and the patient is not an innocent victim of infection, but the organ damage is the results of the patient's active fight with the inducing cause. The authors review recent views regarding the pathophysiology of sepsis.

Humans↗
PubMed1994

Content of the selected trace elements (Al, As, Cd, Cu, Fe, Hg, Mn, Ni, Pb, Zn) in blood, urine and hair of blood donors without occupational exposure to these metals.

The trace element content in biological samples from blood donors (BD) has not been studied in detail so far. In everyday practice minimum attention is paid to the occupational history of blood donors from different social strata. In addition to clinical and elementary haematological and biochemical examinations, the authors assessed levels of Al, As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, and Zn using atomic absorption spectrophotometry (AAS) in two groups of BD--from the Orava (n = 19) and Prievidza region (n = 29). The examined blood donors were never exposed to the risk of metal exposure. No significant differences were found in age and smoking habits between the groups. In analyses electrothermic atomization (AAS-GTA) was mostly used. Hg in urine was assessed, using the technique of hydride vapour formation (VGA). Comparing the results of both BD groups, using Student's t-test, some significant differences between the two regions were found in As, Pb, Cr, Cd, Mn, Ni levels. The authors discuss the possible influence of artificial metal sources from the plants contaminating the environment of the regions for prolonged periods (power plant using coal containing As, metallurgy plant producing ferrochromium and ferromanganese alloys and lead metallurgy plant. Blood levels of metals in BD compared with control groups of the non-exposed population (data obtained from the literature) were within a similar or often lower range. In BD studied very low values of Hg in urine were found (0.015 +/- 0.004 mumol.l-1, 0.021 +/- 0.001 mumol.l-1 of urine -mean +/- SE) with the maximum recorded value of 1.0 mumol Pb.l-1 of blood.

Age Factors↗
PubMed1993

Levels of selected elements (Fe, As, Cd, Pb, Zn, Mn) in biological samples from ferrochromium workers.

A long-term exposure to metals poses the question of whether the impaired health condition is caused exclusively by the quantitatively dominant exposure metal. In 67 workers exposed to ferrochromium alloys on average 12.5 +/- 1.09 years (mean +/- SE), the subgroups of ferrochromium workers and operators of dust removing equipment, average Cr values in urine were 0.233 +/- 0.032 mumol.l-1 (mean +/- SE). In the control group (K) the average Cr values were 0.034 +/- 0.004 mumol.l-1 of urine (mean +/- SE). The levels of plasmatic Fe were assessed photometrically; the levels of Fe, As, Cd, Pb, Mn and Zn in hair, urine and blood were assessed by atom-absorption spectrophotometry (Varian). The results were compared with the control group not significantly different in age (t-test). All the values were correlated with age, years of exposure and the number of cigarettes smoked during life. The values of the single selected metals in furnace workers and operators of dust removing devices were not higher than the limits set for the groups of workers exposed to metals. But compared to the original biological background, there was observed a significant Cd increase (hair, blood, urine) year by year. Average values of As, Pb, Fe, and Mn in hair of the exposed workers were also higher compared to the group K. As and Mn values in hair were increasing up with the years of exposure (r = 0.465, p < 0.05; r = 0.580, p < 0.001). No expected higher values of plasmatic Fe were found. Zn values in hair were falling down with the years of exposure as well as with age, in all groups including the group K. The authors discuss the possible causes of the quantitative changes in the selected metals in connection with professional exposure and non-professional effects (especially smoking). Higher cancer incidence of the organs affected has not been observed so far in the study plant. (Tab. 2, Fig. 8, Ref. 41.)

Adult↗
PubMed1991

[Echocardiographic parameters of the left ventricle in children involved in sports].

The authors investigated, using computer-assisted one-dimensional echocardiography, the influence of systematic two-year sports training on the heart of children engaged in sports (ice hockey n = 20) and a control group of boys not engaged in sports training (n = 25) of the same age (12.6 +/- 0.3 years). At the beginning of the investigation and after two years' training between the two groups no significant differences were found in the following parameters: fractional systolic reduction of the inner diameter of the left ventricle, fractionated systolic enlargement of the interventricular septum, relative width of the left ventricular wall, index of asymetricity of left ventricular hypertrophy and weight of the left ventricle. In none of the investigated parameters the values in sportsmen were beyond the physiological range. The authors did not observe in sportsmen a tendency towards concentric or assymetric hypertrophy of the left ventricle.

Child↗
PubMed1989

[Results of a 2-year study of echocardiographic parameters in juvenile athletes].

The authors investigated, using one- and two-dimensional echocardiography with computer assistance, the influence of systematic sports training on the heart of children engaged in sports (ice hockey, n = 29), as compared with non-training boys of the same age group (n = 25). At the onset of the investigation they did not find any significant differences between the two groups as regards the investigated parameters. After two years training the children engaged in sports had a significantly greater posterior wall thickness of the left ventricle in diastole, as compared with the control group (8.6 +/- 0.8 mm, as compared with 8.1 +/- 0.7 mm, (p less than 0.05) and interventricular septum thickness in diastole (8.6 +/- 0.9 mm, as compared with 8.0 +/- 0.7 mm, p less than 0.05). No significant differences were observed in other dimensional and volume parameters of the left ventricle nor in anthropometric and spirometric parameters.

Child↗