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M W Douglas

Publications and source records attributed to M W Douglas.

25 records · Page 2Linked to original sources
PubMed1999

Dietary formulation with rendered spent hen meals on a total amino acid versus a digestible amino acid basis.

Previous results indicated that performance of chicks fed a high level (15%) of rendered spent hen meal (SHM) was lower than performance of chicks fed a corn-soybean meal (SBM) diet. The latter difference was hypothesized to be due to variation in digestible amino acid (AA) levels among the diets. This study evaluated diets containing SHM formulated on an equivalent total AA basis vs an equivalent digestible AA basis compared to a corn-SBM diet. Three SHM (A, B, and C), produced in commercial rendering plants, were evaluated. Mean true digestibility coefficients (percentages) for 17 AA in SHM A, B, and C were 73.1, 63.4, and 75.5, respectively. In two chick trials, 1-wk-old New Hampshire x Columbian male chicks were fed from 8 to 20 d of age a corn-SBM diet or diets containing 15% SHM formulated to be equal in total or digestible AA to the corn-SBM diet. All diets contained 21.5% CP and 3,200 kcal TME(n)/kg. Chicks fed 15% SHM (A, B, or C) diets formulated on a total AA basis had significantly lower (P < 0.05) weight gain and feed efficiency than chicks consuming the corn-SBM diet. Formulating diets containing SHM B or SHM C on a digestible AA basis yielded growth performance equivalent to chicks fed the corn-SBM diet. Growth performance of chicks fed the SHM A diet formulated on a digestible AA basis was greater (P < 0.05) than that obtained from the SHM A diet formulated on a total AA basis but was still lower than performance of chicks consuming the corn-SBM diet. Additional AA and energy supplementation of the SHM A diet formulated on a digestible AA basis only partially alleviated the latter performance difference. The results of this study indicated that formulation of diets containing SHM on a digestible AA basis is superior to formulation on a total AA basis and that 15% inclusion of two of the three SHM in chick diets had no detrimental effects on performance if the diets were formulated to contain adequate levels of digestible AA.

Amino Acids↗
PubMed1999

Early postmolt performance of laying hens fed a low-protein corn molt diet supplemented with corn gluten meal, feather meal, methionine, and lysine.

Commercial White Leghorn hens (65, 63, or 70 wk of age in Experiments 1, 2, and 3, respectively) were induced molted by feed withdrawal until approximately 28% body weight was lost. All hens were then weighed, and seven replicate groups of 12 hens each were fed molt diets. In Experiment 1, three diets consisted of a corn basal diet (7.9% CP) or this diet supplemented with corn gluten meal (CGM) and Lys or feather meal (FM), Met, and Lys. In Experiments 2 and 3, varying levels of FM and FM with Met and Lys were evaluated. A 16% CP corn-soybean meal diet was used as a positive control in all experiments. The molt diets were fed for 17, 15, and 17 d in Experiments 1, 2, and 3, respectively, and production performance was measured for 8 wk from the beginning of feeding the layer diet. In all experiments, hens fed the 16% CP corn-soybean meal molt diet returned to egg production and regained body weight at a faster rate than did hens fed any of the other diets. In Experiment 1, early egg production of hens fed the corn basal diet supplemented with CGM and Lys or supplementation with FM, Met, and Lys was greater (P < 0.05) than that of hens fed the basal diet alone. In Experiment 2, very early egg production (Week 1) and body weight gain were lower (P < 0.05) for hens fed the corn basal diet than for hens fed the basal supplemented with FM, Met, and Lys. The addition of 5.75 or 8.5% FM or 5.75% FM plus Met and Lys generally increased (P < 0.05) early egg production and postmolt body weight gain compared to the corn basal diet in Experiment 3. The present study thus indicated that improved early postmolt performance may be achieved by supplementation of a low-protein corn molt diet with various combinations of CGM, FM, Met, and Lys.

Animal Feed↗
PubMed1998

Impact of feeding regimen on behavioral and physiological indicators for feeding motivation and satiety, immune function, and performance of gestating sows.

The effect of daily or interval (every 3 d) feeding on body weight change, blood glucose and cholecystokinin (CCK) concentrations, immune function, and behavioral activity were determined during the gestation period of sows. Sows were fed a corn-soybean meal diet either 2 kg daily or 6 kg once every 3rd d (interval). Body weight changes for the 42-d trial period were not different (P > .05) between regimens. Blood glucose concentrations were similar before feeding (P > .05). Two hours after feeding, glucose concentrations increased in interval-fed sows but not in daily-fed sows (P < .05). Premeal plasma CCK concentrations were greater for daily-fed sows than for interval-fed sows (P < .05). The CCK concentrations in sows of both regimens increased after feeding above premeal levels (P < .05), and interval-fed sows exhibited higher concentrations than daily-fed sows (P < .05). Immune function as evaluated through mitogen-induced proliferation of T cells was greater in daily-fed sows than in interval-fed sows (P < .05). Daily-fed sows were more active overall and on any given day than interval-fed sows (P < .05) and thus seemed to expend more energy. Further, daily-fed sows exhibited higher levels of mouth-based activities (i.e., sham chewing, licking, appetitive and consummatory feeding behavior, and excess drinking) than sows restricted to consumption of one large meal every 3rd d. These indicators suggest that feeding motivation significantly affected overall performance of sows. This study emphasizes the need for evaluating the impact of feeding regimens and meal size on feeding motivation and, ultimately, on the well-being of the gestating sows.

Animals↗
PubMed1997

Evaluation of protein and energy quality of rendered spent hen meals.

This study evaluated three spent hen meals (SHM) that were produced in commercial rendering plants. The levels (percentage) of selected nutrients (mean and range) in the meals were: CP, 64 (56 to 71); ash, 16 (12 to 18); lysine, 4.0 (3.6 to 4.3); methionine, 1.3 (1.2 to 1.4); cystine, 1.1 (0.8 to 1.5). Protein efficiency ratios (PER; weight gain per unit of protein intake) were determined in chicks fed 10% CP diets containing a SHM as the only source of dietary protein. The PER values for the SHM averaged 1.6 but varied from 1.1 to 2.2. An experiment was conducted using New Hampshire x Columbian chicks during the 7 to 20 d age period to determine the effect of substitution with either 7.5 or 15% of a SHM to a 21.5% CP corn-soybean meal diet. When substituted at 7.5%, growth performance was not affected by two of the SHM but was depressed by the other one. Growth performance was decreased by all SHM when substituted at 15% of the diet. True amino acid digestibility and TMEn of the SHM were determined using the precision-fed cecectomized rooster assay in which four roosters were crop-intubated with 30 g of a SHM and excreta were collected for 48 h. True amino acid digestibility varied among SHM (P < or = 0.05) and digestibility of cystine was substantially lower than digestibility of the other amino acids. Mean TMEn (kilocalories per gram of DM) of the SHM was 3.003 but varied by 44% among samples. The results of this study indicated that SHM has substantial nutritional value for poultry but the nutritional quality may vary greatly among samples.

Aging↗
PubMed1994

CTL recognition of west Nile virus-infected fibroblasts is cell cycle dependent and is associated with virus-induced increases in class I MHC antigen expression.

Confluent and non-confluent mouse embryo fibroblast (CMEF and NCMEF) monolayers were infected with West Nile virus (WNV) for 24 hr, and class I major histocompatibility complex antigen (MHC-I) concentrations measured by flow cytometry (FCM). Concentrations on CMEF increased significantly more than on NCMEF. This was not owing to differences in interferon-beta (IFN-beta)-mediated MHC induction, as the IFN-beta quantity secreted by each infected fibroblast was similar in each culture, and IFN-beta-mediated MHC-I induction on NCMEF was greater than on CMEF. Furthermore, despite neutralization of WNV-induced supernatant IFN-beta, CMEF increased MHC-I expression significantly more than NCMEF. Functionally, WNV-infected CMEF were lysed 10-fold better by WNV-specific and allospecific cytotoxic T cells, than infected NCMEF. FCM demonstrated 76% CMEF and 68% NCMEF distributed in G0/G1. This represented G0 in CMEF, and G1 in NCMEF, confirmed by ribonucleotide reductase M1 subunit labelling, where only 20% CMEF was labelled, compared to 84% NCMEF. The possible implications for antiviral immune responses are discussed.

Animals↗
PubMed1991

The effects of West Nile virus on major histocompatibility complex class I and II molecule expression by Lewis rat Schwann cells in vitro.

The expression of major histocompatibility complex (MHC) class I and II molecules by Lewis rat Schwann cells after infection with West Nile virus (WNV) in vitro was examined by fluorescence microscopy and flow cytometry. WNV enhanced the expression of MHC class I molecules and induced the expression of MHC class II molecules by Schwann cells. Irradiated medium from WNV-infected Schwann cell cultures upregulated class I molecule expression on dissociated Schwann cell cultures but did not induce the expression of class II molecules. This finding has implications for virally triggered autoimmune diseases of nervous tissue.

Animals↗