PubMed Health⌕ Search

Biomedical subjects

M Walski

Publications and source records attributed to M Walski.

At least 73 records · Page 4Linked to original sources
PubMed1978

Electronmicroscopic histochemical investigation of the surface of the cell membrane of the renal tubules with the use of ruthenium red and lantanum nitrate.

The renal tubules were investigated with the use of ruthenium red and lanthanum nitrate to titrate the carbohydrate groups which are localized on the differentiated surface of the cell membranes and intercellular spaces. Ruthenium red visualized the surface coat which is tightly bound to the outer lamellae of the cell membrane. Lanthanum nitrate used in this investigations is a valuable marker of the intercellular spaces. The applied markers have visualized in the kidney the canals which are originated from the foldings of membranes of the tubular cells which adhere to the basal membrane. The markers used in combination with the fixative glutaraldehyde-paraformaldehyde give an electrondense envelope of the cell membrane which is more distinct as compared with specimen treated with glutaraldehyde and markers only.

Animals↗
PubMed1978

Horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level.

Horseradish peroxidase was used to the precise investigation of low molecular protein transport across the kidney barrier. Simultaneously on the parallel material alkaline phosphatase activity was revealed, as this enzyme is connected with the transport processes through cell membranes. The applied reaction diaminebenzidine has shown infiltration spots for low molecular protein at the glomercular barrier and visualized tubular reabsorption of peroxidase in proximal convoluted tubule. Alkaline phosphatase activity has been observed on the surface of podocytes foot processes. On the surface of proximal convoluted tubules brush border and in canals formed with cells basal membranes investigations high activity of alkaline phosphatase was also observed.

Alkaline Phosphatase↗