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Biomedical subjects

M Warwas

Publications and source records attributed to M Warwas.

At least 19 recordsLinked to original sources
PubMed1989

Cathepsin H from human placenta.

Cathepsin H was isolated from human placenta by autolysis, acetone fractionation, and chromatography on DEAE-cellulose, Sephadex G-75, hydroxyapatite and concanavalin A-Sepharose. The enzyme gave on SDS-polyacrylamide gel electrophoresis two bands of Mr 25,500 and 28,500. Two active forms of the enzyme, with pI of 6.0 and 6.45, were obtained by isoelectric focusing. The enzyme is stable over the pH range 5-7.5, whereas it becomes inactive on heating to 50 degrees C. Cathepsin H of human placenta, like the enzyme from other sources, hydrolyses protein and naphthylamide substrates, showing within the latter group the strongest preference towards arginine-beta-naphthylamide (pH optimum 6.8). The enzyme is inhibited by the known inhibitors of cysteine proteases and by placental cystatins.

Cathepsin H↗
PubMed1989

Cystatins of human placenta.

Two low-molecular protein fractions inhibiting cysteine proteases were isolated from human placenta by alkalization to pH 11, acetone fractionation, affinity chromatography on CM-papain-Sepharose 4B, and Sephadex G-75 gel filtration. The results of polyacrylamide gel electrophoresis, isoelectric focusing indicate that one of there fractions in a dimer of cystatins B, and another is a mixture of cystatins A and B.

Cathepsin H↗
PubMed1987

[Cystatins].

Explore the source record for details and available documents.

Amino Acid Sequence↗
PubMed1986

Haptoglobin and proteinase inhibitors in the blood serum of women with inflammatory, benign and neoplastic lesions of the ovary.

Blood sera of women with myomas of the uterus, benign or malignant neoplasms of the ovary and with inflammatory conditions were examined by determining the haptoglobin concentration and functional activities of: alpha 2-macroglobulin, alpha 1-proteinase inhibitor and alpha-cysteine proteinase inhibitor. In the women with ovarian cancer, as compared with the control group, a significant increase of haptoglobin, alpha 1-proteinase inhibitor and alpha-cysteine proteinase inhibitor was found (p less than 0.001). On the contrary bioactivity of alpha 2-macroglobulin was depressed (p = 0.01). The observed increase of alpha-cysteine proteinase inhibitor activity in the groups with ovarian tumors and ovarian cysts was not related to the inflammatory state as estimated by the haptoglobin concentration.

Adult↗
PubMed1983

Haptoglobin types in ovarian tumors.

Haptoglobin types were determined in 132 patients with cancer of ovaries and 114 patients with nonmalignant tumors of the ovaries, respectively. In comparison with a normal population significant increase in the frequency of Hp1 gene in the both pathological groups was observed. It was mostly reflected in a lower occurrence of haptoglobin type 2-2.

Adolescent↗
PubMed1982

Serum antipapain activity during normal pregnancy.

Antitryptic, antichymotryptic and antipapain activities in maternal serum were measured in the course of normal pregnancy. An increase in inhibitory capacity toward the three examined proteases was demonstrated. The level of antipapain activity was highest between the 13th and 24th weeks of gestation in contrast to antitrypsin and antichymotrypsin activities which have shown the highest values between the 25th and 36th weeks of pregnancy.

Chymotrypsin↗
PubMed1981

Clinical usefulness of serum acute-phase reactants in patients with ovarian tumors.

Concentrations of the following acute-phase reactants (APR) were determined in the sera of patients with benign and malignant ovarian tumors: haptoglobin, ceruloplasmin, sialic acid, seromucoid (in sulphosalicylic acid supernatant), and trypsin inhibitory capacity. In patients with ovarian carcinomas significant increase (p less than 0.001) in all measured APR parameters as compared to healthy women and women with benign tumors was found. Moreover, the levels of haptoglobin, ceruloplasmin and trypsin inhibitory capacity were statistically lower (p less than 0.001) in nonmalignant than in malignant ovarian disease. No distinct statistical difference was confirmed between healthy controls and the benign tumor group as well as between ovarian carcinomas of Stages I--III and Stage IV.

Adenocarcinoma↗
PubMed1979

Serum enzymes in ovarian carcinoma.

The following enzymatic activities were measured in serum of patients with benign and malignant ovarian tumors before treatment: alkaline and acid phosphatases, aspartyl (AspAT) and alanyl (AlAT) aminotransferases, leucyl (LAP) and alanyl (AAP) aminopeptidases, lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase, cathepsin, alkaline ribonuclease (RNase) and beta-glucuronidase. It was shown that at least three determinations (phosphatases and LAP) are practically useless in a discrimination between the examined groups. RNase in combination with AspAT (AlAT) or RNase with AAP and LDH were found to give the best results as marker enzymes.

Adenocarcinoma↗
PubMed1977

Blood serum peptidases in patients with ovarian carcinoma treated with Ledakrin.

The authors observed changes in activity of leucine amino-peptidase (E.C. 3.4.1.1--LAP), gamma-glutamyl transpeptidase (F.C 2.3.2.2--GGTP) and Co++-activated acylase in blood serum of patients with ovarian carcinoma, with or without ascites, treated with Ledakrin (1-nitro-9-[3-dimethylaminopropylamino] acridine). It was stated that the activities of LAP and GGTP increased during the treatment and during tumor progression. The level of Co++-activated acylase increased only slightly during the treatment and therefore, the determination of this enzyme appeared to be of no use in monitoring the therapy of ovarian carcinoma.

Acridines↗
PubMed1977

Activities of some peptidases and glycolytic enzymes in cells of the amniotic fluid. i. Pregnancies terminated prematurely, at term, and postmaturely.

Activities of leucylaminopeptidase, gamma-glutamyl transpeptidase, oxytocinase and aldolase were significantly elevated in the cells of amniotic fluids from cases of premature delivery, compared with cases of delivery at term. The authors conclude that examination of the activities of the aforementioned enzymes can be utilized in prenatal diagnosis of the state of maturity of the fetus.

Amniotic Fluid↗
PubMed1977

Activities of some peptidases and glycolytic enzymes in cells of the amniotic fluid. II. Pregnancies complicated by threatening intrauterine fetal asphyxia.

Activities of leucylaminopeptidase, aspartyl aminopeptidase, gamma-glutamyl transpeptidase, oxytocinase, phosphohexoisomerase, phosphofructokinase and aldolase were studied in amniotic cells from cases of pregnancy complicated by threatening intrauterine fetal asphyxia. Leucylaminopeptidase and oxytocinase activities were much higher in the group of pregnancies complicated by threatening fetal asphyxia than in the control group. It was concluded that elevated activity of the aforementioned enzymes in cells of the amniotic fluid from pregnancies complicated by threatening intrauterine asphyxia is due to acute hypoxia of the fetus.

Adult↗