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Biomedical subjects

M Watabe

Publications and source records attributed to M Watabe.

71 records · Page 4Linked to original sources
PubMed1990

[A study on endoscopic hemostasis from the viewpoint of morphologic traits of Dieulafoy ulcer--an attempt at endoscopic hemostasis by local injection of aethoxysklerol and ethanol].

Characteristics of the vascular course of ruptured vessels in the submucosal layer of Dieulafoy ulcer were studied, and endoscopic hemostatic treatment was performed using Aethoxysklerol and ethanol. 1) Observations of vessel courses in serial sections revealed that ruptured vessels in Dieulafoy ulcer exhibit rhexis in the lateral portions of the vessel and long courses over the ulcer margin in the submucous layer. 2) More dependable hemostasis seemed to be provided by thrombosis not only at the source of bleeding. i.e., at the site of vessel rupture, but also in the region of the whole ruptured vessel in the submucous layer. 3) Hemostatic treatment by local injection of Aethoxysklerol and ethanol was performed in 4 patients with Dieulafoy ulcer, and complete hemostasis was achieved in all 4 patients.

Adult↗
PubMed1989

Identification and nucleotide sequence of the minimal replicon of the low-copy-number plasmid pBS2.

The plasmid pBS2 has a low copy number and is endogenous to Bacillus subtilis. The replication of this plasmid depends on the function of most of the host's dna genes including dnaB, which is unique to B. subtilis and is required for both the initiation of chromosome replication and the DNA-membrane association. We have identified the region that is essential for the replication of pBS2 and determined the complete 2279-bp nucleotide sequence of this region. In this region, there are two stretches of sequence homologous to the 18-bp consensus sequence which commonly appears at the origin of replication of plasmids pUB110 and pC194. The entire region contains six sizable open reading frames. Two of them are probably translated. One open reading frame, designated ORF A, coding for 269 amino acids, has significant homology, in terms of amino acid sequence, with the open reading frame of the gene for the Rep U protein of plasmid pUB110. The similarities between pBS2 and other plasmids suggest that the pBS2 may also replicate as a rolling circle, which appears to be the salient feature of a mechanism of replication that is common to small plasmids in gram-positive bacteria.

Amino Acid Sequence↗
PubMed1983

The influence of age on the induction of delayed hypersensitivity to human gamma globulin.

Delayed hypersensitivity (DH) reactions by chickens to human gamma globulin (HGG) were influenced by age. Chickens 6 to 12 weeks of age exhibited a significantly greater DH reaction than 3-week-old chickens. The injection of HGG 1, 2, or 3 weeks after sensitization did not significantly influence the DH reactions. Our data allow us to reject the hypothesis that there is no significant age difference in the DH response of chickens.

Age Factors↗
PubMed1983

Graft versus host response as influenced by the origin of the cell, age of chicken, and cellular interactions.

Mononuclear cells collected by Ficoll-paque or T cells collected by nylon wool elicited the same magnitude of graft versus host (GvH) response as mononuclear cells collected from the buffy coat (BC). Bone marrow, spleen, or thymic BC cells from day-old chickens did not produce a GvH response. The GvH ability of spleen and bone marrow BC cells increased rapidly during the first 4 weeks and then plateaued. Although at 4 and 12 weeks of age the GvH response produced by thymic BC cells was significantly less than spleen or bone marrow BC cells, thymic BC cells did produce a significant GvH response at these ages. Thymic BC cells from 11-month-old chickens showed significantly higher GvH response than those of previous ages. Corticosterone treatment of chickens eliminated the thymic cortex but did not enhance the GvH reactivity of thymic BC cells. Suppressor effect of thymic BC cells from day-old chickens was not detected. A synergistic effect was noted in the GvH reaction when thymic cells were combined with bone marrow or splenic BC cells of 4-week-old chickens.

Age Factors↗
PubMed1983

Calorie-protein deficiencies and the immune response of the chicken. II. Cell-mediated immunity.

Calorie-protein deficiency affects humoral immunity in the chicken. Secondary responses to sheep-red blood cells, a T-dependent antigen, were lower in birds fed diets two-thirds deficient in calories (C), amino acids (AA), or both (C/AA). These results led us to question what effects might be found in cell-mediated immunity after nutritional deficiency. Birds fed the same diets as in our previous study showed no reduction in total white blood cells, absolute lymphocytes, or absolute heterophils. Splenic lymphocytes from 3- or 7-week-old nutritionally deficient chickens with the exception of the AA group at 7 weeks, produced a nonsignificant increase in graft-versus-host (GvH) response of 12-day-old embryos. Thymic cells from 3-week-old nutritionally deficient birds were not deficient in the GvH potential. Thymic histology revealed reduced cellularity in AA-deficient birds. The reduced cellularity may not have been in response to stress since the mean corticosterone levels were not significantly different. Birds fed the deficient diets for 10 weeks had a significantly reduced delayed hypersensitivity response to human gamma globulin. The results indicate that the chickens' ability to produce a GvH response was not compromised and that T-cell subpopulations may have differential sensitivities to nutritional deficiencies.

Amino Acids↗
PubMedDate not supplied

Preparation, 195Pt NMR spectra and biological activity of platinum (IV) complexes with dipeptides.

Three dipeptide complexes of the form K[Pt(IV) (dipep) Cl(OH)2] and four dipeptide complexes of the form K[Pt(IV)-(Hdipep)Cl2(OH)2] were newly prepared. The 195 Pt NMR peak of the K[Pt(IV) (dipep)Cl(OH)2] complexes appeared at about 1200 ppm and these chemical shifts were about 3150 ppm downfield compared with those of the K[Pt(II) (dipep) Cl] complexes. The chemical shifts of the K[Pt(IV) (Hdipep) Cl2 (OH)2] complexes were at about 900 ppm, i.e., about 3050 ppm downfield compared with those of the K[Pt(II) (Hdipep)Cl] complexes. The H[Pt(IV) (Hdigly) Cl2(OH)2] and K[Pt(IV) (Hdigly) Cl2(OH)2] complexes inhibited the growth of C. albicans at a more diluted concentration than cisplatin at 1 microgram/ml, but the platinum complexes only weakly inhibited the growth of these cells compared with the cisplatin-inhibited growth of Meth-A and Hep-2 cells at 10 micrograms/ml. These results suggested that the platinum complexes selectively inhibited the growth of fungal cells.

Anti-Bacterial Agents↗
PubMedDate not supplied

Cell cycle arrest and protein kinase modulating effect of bufalin on human leukemia ML1 cells.

Bufalin, an active principle of the traditional Chinese medicine chan'su, has been proved to be a potent differentiation inducer in human leukemia cells. To study the mechanism of the differentiation of human leukemia ML1 cells induced by bufalin, we measured the effect of 10 nM bufalin on cell growth, activities of various protein kinases, and cell cycle. The ML1 cell growth was inhibited significantly at 24 hr and the inhibiting effect persisted for 6 days. Activities of PKC, PKA, cdc2 kinase and CK II in ML1 cells were changed early by bufalin; PKA and PKC activities were inhibited, and cdc2 kinase and CK II activities were increased. These results suggest that bufalin induces differentiation of ML1 cells by modulating several protein kinase activities in a distinct way from RA and 1 alpha, 25(OH) 2D3. Cell cycle changes, measured by flow cytometry, became evident at 12 hr after treatment of ML1 cells with bufalin and the cells were preferentially arrested in the G2/M phase. This effect of bufalin on the cell cycle of leukemia cells is similar to that of topoisomerase inhibitors. Indeed, the activity of topoisomerase II but not topoisomerase I of ML1 cells was inhibited remarkably by the treatment of the cells with 10 nM bufalin.

Bufanolides↗