Biomedical subjects

M Watsky

Publications and source records attributed to M Watsky.

3 recordsLinked to original sources

PubMed1992

Potassium currents from isolated frog lens epithelial cells.

Using the perforated patch version of whole-cell recording, we have measured currents from isolated frog lens epithelial cells. Three types of currents were seen. A time-independent outwardly rectifying potassium current was identified that sets the resting voltage. This potassium current differs significantly from any of the potassium currents recorded with the whole-cell technique in mammalian lens epithelial cells. In addition to the potassium current, the two other currents present were both outwardly rectifying: one was time-independent while the other showed distinct activation.

Animals↗

PubMed1991

Low access resistance perforated patch recordings using amphotericin B.

We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

Amphotericin B↗

PubMed1976

Psoriasis: topical corticosteroid therapy.

Fifty-nine patients with bilateral psoriatic lesions were subjects in a double-blind paired comparison of halcinonide cream and fluocinonide cream. They applied the corticosteroid creams twice a day for 14 days without occlusive dressings. Objective and subjective assessment of response to therapy was made four or five days, one week, and two weeks after starting treatment. The results were analyzed by statistical methods. At the second visit (day 4 or 5), there was little difference between the two compounds, but at visits three and four, reduction of inflammation was significantly better with halcinonide than with fluocinonide.

Adult↗