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Biomedical subjects

M Whiting

Publications and source records attributed to M Whiting.

16 recordsLinked to original sources
PubMed1992

Detection of Pediococcus spp. in brewing yeast by a rapid immunoassay.

A membrane immunofluorescent-antibody test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelleted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in less than 4 h. The sensitivity of the assay is 2 orders of magnitude below the Pediococcus detection limit of direct microscopy.

Fluorescent Antibody Technique↗
PubMed1984

Infantile Refsum's disease (phytanic acid storage disease): a variant of Zellweger's syndrome?

The activity of phytanic acid oxidase is low in infantile and adult Refsum's disease, and in the cerebro-hepato-renal (Zellweger's) syndrome. The plasma of patients with the infantile but not the adult form of Refsum's disease contains increased amounts of pipecolic acid and of at least two abnormal bile acids, one of which has been identified as 3 alpha, 7 alpha, 12 alpha trihydroxy-5 beta-cholestan-26-oic acid. These changes are similar to those reported in the Zellweger syndrome and indicate that there may be similarities in the metabolic defects in Zellweger's syndrome and the infantile form of Refsum's disease.

Abnormalities, Multiple↗
PubMed1977

Neural influence on experimental myotonia.

A transitory myotonic state, lasting for less than 24 hours, was induced in rats within 2 hours of the intraperitoneal injection of 2,4-dichlorophenoxyacetic acid. Neuromuscular blockade, nerve block, nerve section, and cordotomy had no effect on the established myotonic state. However, muscle that had been denervated progressively lost its capacity for a myotonic response to subsequent injections of 2,4-dichlorophenoxyacetic acid. After 10 to 12 days denervation, the injection of 2,4-dichlorophenoxyacetic acid produced a brief increase in the number of fibrillations, but typical myotonic discharges were no longer detectable. Unlike denervation, cordotomy did not impair the capacity of the paralyzed limbs to develop in response to 2,4-dichlorophenoxyacetic acid. While myotonia is the result of a primary defect in the muscle membrane, these experiments indicate the innervation is necessary to maintain the muscle membrane in a state that will support myotonia. We hypothesize that an alteration in ionic conductance secondary to denervation is the critical factor in preventing myotonia.

2,4-Dichlorophenoxyacetic Acid↗
PubMedDate not supplied

The role of nurses in health purchasing.

This paper considers nurses' responsibilities on giving advice about the purchasing of health care. By outlining the steps in the purchasing cycle, a coherent framework is presented for identifying the specific issues associated with the purchasing of health services by district health authorities (DHAs), and family health service authorities (FHSAs) in England and Wales, and health boards (HBs) in Scotland. Purchasing health is every nurse's business. All registered nurses in the UK have a contribution that they can and should make to ensure that their DHA, HB or FHSA makes the best possible provision for their resident population.

Community Health Services↗