Biomedical subjects

M Wilsson-Rahmberg

Publications and source records attributed to M Wilsson-Rahmberg.

3 recordsLinked to original sources

PubMed2001

Permanent jejunal fistula: promising method for obtaining small intestinal chyme without disturbing intestinal function.

Accurate information on changes in small intestinal microflora in dogs is rather limited because of difficulties in obtaining samples of small intestinal chyme. In the study reported here, intussuscepted nipple valves were surgically placed into the jejunum of seven laboratory beagles to obtain intestinal juice samples. The influence of the fistula on intestinal motility was determined by use of barium-impregnated polyethylene spheres (BIPS) and on microflora by use of bacterial culturing. The BIPS were fed two weeks before surgery and again five weeks after surgery. Bacterial samples were collected before (fecal samples), during (small intestinal samples) and 11 weeks after surgery. There were no surgical complications, and the animals tolerated the fistula well. Mean orocolic transit percentage was 93% before and 83% after surgery, and notable changes in gastrointestinal motility were not seen, except in one dog. The surgery did not markedly alter the bacterial flora in feces. Microflora did change in small intestinal samples; however, methodologic factors may explain most of these differences. In conclusion, the nipple valve is a promising method that creates easy and safe long-term access to the jejunum and appears not to have an influence on intestinal function.

Animals↗

PubMed1997

Method for long-term intestinal access in the dog.

A variety of devices have been used to establish long-term intestinal access in laboratory animals. Their use is complicated by infection, tissue reaction, and a frequent need for single housing to prevent implant damage. Intussuscepted nipple valves have long been used in the human field of surgery for various applications where there is a need to create a reservoir without leakage. With the use of the nipple valve it was possible to establish long-term intestinal access in dogs with minimal post-operative complications, no leakage and, because no foreign material was used, no complications due to tissue rejection. The advantages of a nipple valve over the similar technique of a straight stoma is less postoperative complications in the form of leakage as well as enhanced access due to the design of the nipple valve. Encouraging results prompted us to widen the use of nipple valves to 14 dogs in which it has been possible to establish long-term colonic or duodenal/ileal access at one or two sites. Nine of the dogs are still in use with duration of instrumentation for some animals exceeding 60 weeks. This method requires minimal maintenance, does not prevent group housing of instrumented dogs or outdoor activities nor does it compromise the quality of life for the animals.

Anesthesia↗

PubMedDate not supplied

Method for long-term cerebrospinal fluid collection in the conscious dog.

In medical research there is often a need for cerebrospinal fluid (CSF) collection from conscious animals. A number of models have been published, but long-term use is often limited by factors such as damage to implant or loss of patency. When part of an implant is exteriorized, group housing is often not possible; even when singly housed, the instrumented animals might have to be fitted with protective devices such as collars, vests, or helmets. Over the last 10 years we have, through the use of this technique, managed to perform repeated CSF collections in 12 beagles. Time of implant duration has ranged from 2.3 to 6.8 years. The method uses two permanent stainless steel guide cannulas screwed into the parietal bone, positioned above but not penetrating the lateral ventricles of the brain. At the time of collection, one of the subcutaneous stainless steel cannulas is used to serve as a guide for a 20-gauge collection needle. The collection is performed under local analgesia and with minimal discomfort to the dog. With its long-term use, we feel that this model is very useful and of high ethical and welfare standards due to the virtual lack of postoperative complications, maintenance requirements, and housing restrictions.

Animals↗