[Extracorporeal shockwave lithotripsy].
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Anesthesia, Conduction↗
Biomedical subjects
M Wirth
Publications and source records attributed to M Wirth.
Blood pressure lowering effect of eicosapentaenoic acid-rich diet in normotensive, hypertensive and hyperlipemic subjects.
A mackerel diet or a herring diet in which two cans of fish fillet were consumed daily over 2 weeks within a prescribed regimen, in a crossover design, were given to 15 normotensive volunteers, 14 patients with mild essential hypertension and eight patients with type IV and V hyperlipoproteinemia (HLP). In normotensives a markedly lower systolic and diastolic blood pressure at the end of the period on the mackerel diet could be observed, whereas in hypertensive and hyperlipemic subjects only systolic blood pressure was significantly decreased. After the herring diet, which served as control, changes in blood pressure were of a minor degree.
Adult↗
CHOP-firstline treatment in NHL with unfavorable prognosis--evaluation of therapeutic response and factors influencing prognosis.
58 NHL-patients (9 large cell centrocytic, 18 centroblastic, 16 immunoblastic, 15 lymphoblastic lymphomas) were treated immediately after diagnosis with CHOP-chemotherapy regardless of the extent of disease. Because of the advanced age of the majority of patients (median age 61 years, range 22-85 years) a reduced dose in the first two cycles was administered. Statistically significant prognostic variables influencing survival were the following: histologic subtypes according to the Kiel-classification (p less than 0,05), B-symptoms (p less than 0,001), blood sedimentation rate (p less than 0,02) and LDH (p less than 0,0005). With regard to prognosis there was no difference between patients over 60 years of age and younger ones (p less than 0,4). Patients achieving complete remission survived significantly longer (p less than 0,0001). Ann Arbor stages were of limited value, since patients with CS II disease and accumulation of risk factors (B-symptoms, abdominal disease, bulky tumor masses) showed a poorer outcome than patients with CS III who did not have these risk factors. A risk factor score summarizing features influencing prognosis is described and might be a useful tool in stratifying the heterogeneous group of NHL with unfavorable prognosis.
Adult↗
Blood pressure- and lipid-lowering effect of mackerel and herring diet in patients with mild essential hypertension.
Fourteen male patients with mild essential hypertension were put on a mackerel and herring diet within a prescribed isocaloric regimen in a cross-over design for 2 weeks. After mackerel diet eicosapentaenoic acid (EPA-C20:5, n-3) appeared more in cholesterol esters (1.7-11.0%), whereas docosahexaenoic acid (DHA-C22:6, n-3) was predominantly incorporated into serum triglycerides (1.0-8.3%). After herring diet, which contained half as much EPA and DHA, their increase was of minor degree. After mackerel diet serum triglycerides, total cholesterol, LDL cholesterol and lecithin cholesterol acyl transferase (LCAT) activity were significantly decreased (by 28%, 9%, 14% and 14%, respectively), returning to the initial levels 3 months later. On the contrary, HDL cholesterol appeared significantly increased (by 12%). After herring diet the differences were not significant. Serum sodium was significantly lower (by 2%) at the end of the mackerel diet as compared to the initial values. On the other hand, uric acid in serum appeared transiently increased (by 24%) at the end of both dietary periods. A significant decrease (by 8%) in casual systolic blood pressure, measured in recumbent position, could be observed only at the end of the mackerel period. Moreover, the level of systolic and diastolic blood pressure before and during a standardized psychophysiological stress test was significantly lower after mackerel diet. Nevertheless, the increments after stress were similar. Plasma renin activity was increased (by 64%) after mackerel diet.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗
Influence on serum lipids, lipoproteins and blood pressure of mackerel and herring diet in patients with type IV and V hyperlipoproteinemia.
Eight patients with type IV and V hyperlipoproteinemia were put on a mackerel and herring diet of an isocaloric regimen for 2 weeks, in a cross-over design. At the end of the dietary periods a predominant increase of eicosapentaenoic acid (EPA - C20:5, n-3) in cholesterol esters and of docosahexaenoic acid (DHA - C22:6, n-3) in serum triglycerides, being more pronounced after mackerel as compared to herring diet, could be confirmed. After mackerel diet serum triglycerides and total cholesterol were significantly lower, returning to basal levels 3 months later. High density lipoprotein (HDL) cholesterol appeared slightly increased after mackerel diet and decreased to initial values thereafter. After herring diet, which contained half as much EPA as compared to mackerel diet, the differences were minor. The decline of free fatty acids (FFA) and insulin at the end of the mackerel period reached the level of significance 60 min and 120 min, respectively, after glucose load. A significantly lower systolic blood pressure in recumbent and upright position after the mackerel period could be found, whereas diastolic pressure and blood pressure after herring diet remained unchanged.
Adult↗
Functional properties of natural killer cells in carcinoma of the prostate.
Functional properties of natural killer cells were analyzed in a 51chromium-release assay by modulating their activity with human beta-interferon using 1) peripheral mononuclear cells of 16 patients with carcinoma of the prostate and 7 healthy male donors, and 2) mononuclear cells from the periprostatic lymph nodes of 6 patients with stage pT2N0M0 prostatic cancer and 5 without malignancy. Cell lines EB 33, PC 3, DU 145 and CaKi 1 were used as target cells. Spontaneous cell-mediated cytotoxicity of peripheral mononuclear cells was depressed in patients with advanced prostatic cancer; however, the natural killer cells responded as those of the healthy controls to beta-interferon. The beta-interferon effect was time and dose dependent. In mononuclear periprostatic lymph node cells virtually no spontaneous cell-mediated cytotoxicity was detected. The reactivity of mononuclear periprostatic lymph node cells from patients with prostatic cancer was significantly less improved by beta-interferon stimulation than the mononuclear periprostatic lymph node cells of healthy subjects. The stimulation of mononuclear periprostatic lymph node cells by beta-interferon was reduced significantly compared to simultaneously tested autologous peripheral mononuclear cells.
Adult↗
[Transurethral punch resection of the prostate (cold punch). Results and complications].
In 1965 the cold punch technique of transurethral prostatic resection was introduced in Germany. The principal differences between the electroresectoscope and the punch resectoscope are explained. This report on the results and complications of cold punch resection of the prostate is based on 3030 patients who were operated upon by this technique. The average age was 71,6 years. 8,2% of the patients were 80 years or older. In 33,6% the operative risk was increased. Intraoperative, early and late complications of the procedure are reported. The mortality of 1,4% over the entire 20 year period was decreased to 0,65% for the last 10 years.
Aged↗
Prevention of amiodarone-induced photosensitivity.
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Amiodarone↗
Glycoprotein E1 of MHV-A59: structure of the O-linked carbohydrates and construction of full length recombinant cDNA clones.
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Animals↗
Age-dependent alterations of linoleic, arachidonic and eicosapentaenoic acids in renal cortex and medulla of spontaneously hypertensive rats.
The lipid content as well as the fatty acid pattern of triglycerides, free fatty acids (FFA), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were estimated in renal cortex and medulla of spontaneously hypertensive rats (SHR) and normotensive Wistar rats (WR) at 4, 8, 26 and 52 weeks of age. In general, the level of triglycerides in renal medulla appeared higher when compared with the cortex. On the other hand, PC and PE, increasing with age, were usually higher in the cortex. A decreased percentage of linoleic acid (LA) in triglycerides, of arachidonic acid (AA) in PC and of eicosapentaenoic acid (EPA) in triglycerides, FFA, PC and PE could be found in the kidneys of SHR at 8 weeks of age, i.e. during the development of hypertension. This was accompanied with a rise of AA in FFA of SHR at 8 weeks of age, which occurred with delay in WR (at 26 weeks of age). From the data presented it can be concluded that systematic alterations in the availability of individual polyunsaturated fatty acids (PUFA) in various renal lipids might be related to the onset of hypertension in SHR which should be elucidated in more detail.
Aging↗
Changes of arachidonic acid in phosphatidylcholine different from other lipids in kidneys of spontaneously hypertensive rats after feeding a linoleic acid-rich diet.
In spontaneously hypertensive rats (SHR) fed a linoleic acid-rich (LAR) diet over 22 weeks blood pressure could not be influenced when compared with SHR on linoleic acid-deficient (LAD) food. On the other hand, normotensive Wistar rats (WR) revealed a significantly lower blood pressure after feeding a LAR diet. The percentages of LA and AA were markedly increased in triglycerides and free fatty acids (FFA) in renal cortex and medulla of LAR-fed SHR and WR in comparison to LAD-fed animals. In renal phosphatidylethanolamine (PE) LA was also increased whereas AA remained unchanged. On the contrary, in phosphatidylcholine (PC) LA was only slightly augmented (in SHR) or even lowered (in WR), AA appearing decreased in SHR but increased in WR. These different changes in PE and PC indicate that individual phospholipids show a dissimilar behavior after dietary interventions, which can be masked by the evaluation of total phospholipids and might be of pathophysiological relevance.
Animals↗
Decrease of arachidonic acid in phosphatidylcholine from renal medulla of SHR after feeding a linoleic and linolenic acid-rich diet.
Linoleic acid-rich (LAR), linolenic acid-rich (LNAR) and linoleic acid-deficient (LAD) diets in spontaneously hypertensive rats (SHR) and normotensive rats (NR) resulted in various changes in n-6 and n-3 fatty acids of lipids in renal medulla. After LAR diets the percentage of linoleic (LA) and arachidonic acids (AA) was high only in triglycerides (TG) and free fatty acids (FFA). In phosphatidylethanolamine (PE) LA was increased after LAR diet, whereas AA remained unchanged. In phosphatidylcholine (PC) LA was higher, but AA appeared lower as compared to LAD fed SHR. After LNAR diet linolenic (LNA) and eicosapentaenoic acids (EPA) were increased, but AA appeared significantly lower. Consequently, the lowest percentage of AA was found after LNAR diet. Blood pressure could not be influenced by the diets.
Animals↗
Alterations of linoleic, arachidonic and eicosapentaenoic acids in renal cortex and medulla of SHR during the onset of hypertension.
104 spontaneously hypertensive rats (SHR) and 107 normotensive control rats (WR) fed a commercial diet were sacrificed at 4, 8, 26 and 52 weeks of age. The kidneys were immediately removed and carefully separated into cortex and medulla. In general, the triglyceride levels in renal medulla appeared higher when compared with the cortex. On the other hand, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were usually higher in the cortex and increased with age. In the kidneys of SHR at 8 weeks of age, i.e. during the development of hypertension, the percentage of linoleic acid (LA) in triglycerides and of arachidonic acid (AA) in PC was decreased. This was associated with an increase of AA in free fatty acids (FFA), indicating an enhanced activity of phospholipase A2. Furthermore, a fall of eicosapentaenoic acid (EPA) in triglycerides, FFA, PC and PE of kidneys in SHR at 8 weeks of age could be found. The data suggest that systematic alterations in the availability of individual polyunsaturated fatty acids in various renal lipids might be related to the onset of hypertension in SHR. Age-dependent changes in enzymes involved in prostaglandin formation should be considered as an adaptive mechanism which is insufficient to reduce or abolish genetically determined hypertension in SHR.
Aging↗
Clinical studies on lipid and blood pressure lowering effect of eicosa-pentaenoic acid-rich diet.
15 volunteers were put on a mackerel (MD) and herring diet (HD) for 2 weeks in a cross-over design. At the end of the dietary periods eicosa-pentaenoic acid (EPA) was predominantly incorporated into cholesterol esters (CE), whereas docosahexaenoic acid (DHA) appeared more in triglycerides (TG). This was more pronounced after MD as compared to HD. After MD a significant decrease of serum TG, total cholesterol (C) and lecithin cholesterol acyl transferase (LCAT) activity could be observed returning to the initial levels 3 months later. After HD only a decrease of LCAT was significant. In addition, a marked decrease in blood pressure (BP) was associated with lower plasma noradrenaline levels after MD, but not after HD. In 14 patients with mild essential hypertension an enhanced percentage of EPA and DHA in the lipids of VLDL, LDL and HDL could be confirmed. After MD serum TG, C, LDL-C and LCAT were significantly decreased, whereas HDL-C appeared increased. After HD the changes were of minor degree. Systolic BP was significantly lower at the end of MD. In 8 patients with type IV and V hyperlipoproteinemia (HLP) similar changes could be seen, the most striking finding being a fall in serum TG, especially if initial values were extraordinarily high. The data suggest a beneficial effect of MD on several parameters known as risk factors of atherosclerosis.
Adult↗
The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1.
Two size classes of O-glycosidically linked oligosaccharides were liberated from glycoprotein E1 of mouse hepatitis virus (MHV) A59 by reductive beta-elimination and separated by h.p.l.c. The structures of the reduced oligosaccharides were determined by successive exoglycosidase digestions and by methylation analyses involving combined capillary gas chromatography-mass spectrometry and mass fragmentography after chemical ionization with ammonia. Oligosaccharide A (Neu5Ac alpha 2----3 Gal beta 1----3 GalNAc) comprised 35% of the total carbohydrate side chains, while the remaining 65% of the oligosaccharides of E1 had the branched structure B: Neu5Ac alpha 2----3 Gal beta 1----3 (Neu5Ac alpha 2----6) GalNAc. Both oligosaccharides were linked to the E1 polypeptide via N-acetylgalactosamine, and 20% of the sialic acids present in E1 glycopeptides were found to consist of N-acetyl-9-mono-O-acetylneuraminic acid. The reported structures of the O-linked glycans are discussed in the context of the amino acid sequence of E1, which exhibits a cluster of four hydroxyamino acids (Ser-Ser-Thr-Thr) as potential O-glycosylation sites at the amino terminus. Oligosaccharides with identical structures and an identical O-glycosylated tetrapeptide sequence are present in the blood group M-active glycophorin A of the human erythrocyte membrane.
Carbohydrate Sequence↗
Acridine-psoralen amines and their interaction with deoxyribonucleic acid.
A series of novel compounds in which a 9-acridinyl nucleus is linked to a psoralen nucleus in the 5- or 8-position via polyamines was prepared and examined. Their reversible binding to DNA and their irreversible binding to DNA and DNA cross-linking upon irradiation with UV-A light were examined. It was found that they were all less efficiently photoreactive than 8-methoxypsoralen (8-MOP), both in cross-linking and photobinding to DNA, whereas the ratio between their photobinding and cross-linking was 40-400 times that of 8-MOP. Compounds in which the linker was attached to the 5-position in psoralen showed smaller cross-linking and photobinding efficiencies and larger ratios between photobinding and cross-linking than those of psoralens attached in the 8-position. This strongly indicates that the 9-substituents of the acridines are oriented toward the minor groove. Flow linear dichroism studies showed that the compounds were DNA intercalating with the acridine moiety, whereas the psoralen moiety in no case was clearly intercalating. This conclusion was further supported by viscometry which also strongly indicated monointercalation.
Acridines↗
Lipid and blood pressure-lowering effect of mackerel diet in man.
Fifteen healthy volunteers were put on a mackerel and herring diet, consisting of a prescribed daily isocaloric regimen in a cross-over design, for 2 weeks. Eicosapentaenoic acid (EPA - C20:5, n-3) was predominantly incorporated into cholesterol esters, whereas docosahexaenoic acid (C22:6, n-3) appeared more in serum triglycerides, indicating that the function of the latter may be different from that of EPA. After mackerel ingestion, serum triglycerides, total cholesterol and lecithin cholesterol acyl transferase (LCAT) activity were significantly decreased, returning to basal levels 3 months later. Low density lipoprotein (LDL) cholesterol, high density lipoprotein (HDL) cholesterol and postheparin lipolytic activity (PHLA) remained unchanged at the end of the mackerel diet. Generally, after the herring diet the differences were minor, only LCAT activity being significantly decreased. A markedly lower systolic and diastolic blood pressure at the end of the mackerel period could be observed. After herring diet a slight diminution of blood pressure was not significant. Accordingly, plasma noradrenaline was only significantly decreased at the end of the mackerel period. Dopamine-beta-hydroxylase (DBH) activity in serum had no differences before, during and after the study. From the data presented it can be said that a mackerel diet exerts a beneficial influence on cardiovascular risk.
Adult↗
Interaction between glucose and dehydroascorbate transport in human neutrophils and fibroblasts.
Dehydroascorbate (DHA) competitively inhibits the uptake of 2-deoxy-D-glucose and of 3-O-methyl-D-glucose by human blood neutrophils; 2-deoxyglucose and 3-O-methylglucose competitively inhibit dehydroascorbate uptake. Ratios of Km to Kl are near unity for each of these ligands. Exposure of cultured human fibroblasts to insulin enhances cytochalasin B-inhibitable uptake of DHA and of 2-deoxyglucose, both to the same degree. These observations suggest that DHA transport is mediated by glucose transport systems in human neutrophils and fibroblasts.
3-O-Methylglucose↗