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M Wolk

Publications and source records attributed to M Wolk.

28 records · Page 2Linked to original sources
PubMed1980

Evaluation of standard curves in the graded hemagglutination-inhibition method applied to quantitation of human immunoglobulins.

For accurate quantitation of human immunoglobulins A and B by graded hemagglutination inhibition, I have established standard curves. For IgA, plotting the inner diameter of the ring formed by sedimented erythrocytes vs the log of the antigen concentrations gives a straight line. Plotting the same parameters for IgG gives a hyperbolic line. These ratios between antigen concentration and ring diameter were constant for at least one year. Both undiluted and 10,000-fold diluted human serum may be quantitated by this method, depending only on immunoglobulin concentrations. Precision (CV) of the system, assessed in 35 patients' sera over the period of one year, ranged from 0.5 to 15.2% (mean 10%) for IgA and from 1.4 to 14.9% (mean 7.3%) for IgG.

Hemagglutination Inhibition Tests↗
PubMed1975

Cerebrospinal fluid acidosis complicating therapy of experimental cardiopulmonary arrest.

Cardiopulmonary resuscitation (CPR) may be followed by slow recovery of brain function. The possible role of bicarbonate therapy was assessed by analysis of arterial blood and cerebrospinal fluid (CSF) in 20 dogs during cardiac arrest and CPR. Samples were taken in the control period and every 5 minutes post-arrest of 20 minutes. Group I received no post-arrest CPR. Arterial pH fell from 7.37 to 7.31 (P less than 0.01) and CSF pH from 7.34 to 6.94 (P less than 0.001). Arterial pCO2 rose from 39 to 65 mm Hg (P less than 0.005) and CSF pCO2 increased from 47 to 123 (P less than 0.02). With CPR alone (group II) arterial pH decreased from 7.39 to 7.19 (P less than 0.005), while arterial pCO2 and CSF pH and pCO2 were undhanged. CPR with bicarbonate therapy (mEq = weight in kg times 0.43 times 1.1 mEq/min of arrest) given every 5 minutes (group III), resulted in a rise in arterial pH from 7.41 to 7.81 (P less than 0.02). Excess bicarbonate administration during CPR may result in a marked dissociation between arterial and CSF pH as a consequence of rapid CO2 diffusion across the blood-brain barrier. Large ampounts of NaHCO3 given during CPR may contribute to post-CPR cerebral depresssion.

Acid-Base Equilibrium↗
PubMedDate not supplied

Percutaneous cardiopulmonary bypass to support angioplasty and valvuloplasty. Technical considerations.

To eliminate problems during percutaneous transluminal coronary angioplasties (PTCAs) or valvuloplasties (PV), percutaneous cardiopulmonary bypass (PCPB) was used in 19 high-risk patients (pts) to perform supported PTCA (12 pts, age 40-68) for low EF and/or a large area of risk (L main), and supported PV (7 pts, age 68-92) for "nonoperative" candidates with severe AS. Our experience promoted a review of the technical aspects of PCPB was needed for a successful outcome in these high-risk patients. All patients were treated with IV amnestics while awake in the catheter laboratory. Early in the experience, 20F arterial and venous cannulas were inserted via a 2 cm cutdown in the right groin; now 17F arterial and 20F venous cannulas are placed via a Seldinger technique using fluoroscopy. Traversement of the iliac vein is enhanced by a roll under the buttocks. If the artery is calcified, a cutdown, vessel loops, and direct repair are employed to assure post-PCPB patency. Total flows of 6L/min are easily achieved with nonreservoir centrifugal pump bypass. If flow or pressures fall, 200 cc of blood or crystalloid rapidly improves venous drainage and flows and unlike during reservoir bypass, alpha agonists are only rarely necessary; CaCl often produces nausea in these patients. PCWP's are brought to 0-5 mm Hg. Due to the 1,500 cc priming volume, on-pump transfusion is often required. Two patients were successfully transported to the OR on PCPB for treatment of catheter failures. With attention to technical details, percutaneous bypass supported PTCA or PV is an important new therapy for high risk patients.

Adult↗