PubMed Health⌕ Search

Biomedical subjects

M Wysocka

Publications and source records attributed to M Wysocka.

71 records · Page 4Linked to original sources
PubMed1988

Limiting dilution analysis of memory cytotoxic T lymphocytes specific for individual influenza virus gene products.

The frequency of memory T cells specific for individual influenza virus gene products in the spleens of BALB/c mice primed with A/Puerto Rico/8/34/1(H1N1) (PR8) influenza A virus was determined by using limiting dilution protocols. The results confirm that the influenza virus nucleoprotein (NP) is the predominant protein recognized by BALB/c cytotoxic T lymphocyte (CTL). This was true whether the responder splenocytes were restimulated with homologous PR8 virus or heterologous A/Japan/305/57 (H2N2) (JAP). The frequency hierarchy for BALB/c splenocytes restimulated with PR8 was NP greater than PB2 greater than or equal to H1 greater than NS1 greater than H2. The frequency of CTL specific for all other influenza gene products was too low to calculate. The CTL hierarchy for BALB/c splenocytes restimulated with JAP was NP greater than NS1, with all others lower than 1 in 96,000.

Animals↗
PubMed1986

Quantitative analysis of tissue distribution of Ly10-like murine alloantigen(s) with antisera and monoclonal antibodies.

Tissue distribution of non-Lyt1.1 ("Ly10-like") antigen or antigens encoded by short chromosomal segment differentiating B6-Ly-1a congenic strain from B6 strain of mice was studied by quantitative absorption of (BALB/c X B6)F1 anti B6-Ly-1a antiserum and by direct cytotoxicity of Ly-10-132-12-26 monoclonal antibody on lymphoid cell populations. Identical strain but not tissue distribution pattern does not allow to conclude whether antiserum and monoclonal antibody detect the same or closely linked antigens. Absorption experiments revealed the highest antigen content in the brain tissue, lower in testis and kidney, still lower in lymphoid organs and the lowest in liver and lung. Among lymphoid cells, bone marrow cells had highest absorbing capacity, followed by thymus, spleen and lymph nodes. Monoclonal antibody lysed almost 100% of thymocytes, 30% bone marrow cells and 10-20% of spleen and lymph node cells (both T-cell and B-cell enriched populations contained the same proportion of positive cells). Cortisone resistant thymocytes showed the same sensitivity as cortical thymocytes to Ly-10-132-12-26 antibody which is distinguishable characteristics of medullary thymocytes from peripheral T cells. Mitogen activated lymphocytes exhibited significantly higher expression of Ly10-like antigen than resting peripheral lymphocytes.

Animals↗
PubMed1985

Normal salivary mucin contains the gastrointestinal cancer-associated antigen detected by monoclonal antibody 19-9 in the serum mucin of patients.

Monoclonal antibody 19-9 produced by a hybridoma prepared from spleen cells of a mouse immunized with a human colon carcinoma cell line, detects an antigen in sera from many patients with gastrointestinal and pancreatic cancer. This antibody is directed against a carbohydrate with the sugar sequence (Formula: see text) In many gastrointestinal and pancreatic cancers this sequence occurs in a monosialoganglioside containing a sialylated Lea-active pentasaccharide (sialylated lacto-N-fucopentaose II, IV3-alpha-NeuNAc-III4-alpha-Fuc-LcOse4). In the sera of cancer patients the antigen occurs as a mucin. The antigen is also found in salivary mucin from many normal individuals belonging to the Le(a+b-) or Le(a-b+) blood group but not in salivary mucin from normal individuals belonging to the Le(a-b-) blood group. There is more antigen in mucin from individuals belonging to the Le(a+b-) blood group than in mucin from individuals belonging to the Le(a-b+) blood group.

Antibodies, Monoclonal↗
PubMed1984

Monoclonal antibody localization of Lewis antigens in fixed tissue.

Monoclonal antibodies that bind specifically with Lewisa (Lea) and Lewisb (Leb) antigens were used in an immunoperoxidase assay to characterize Lewis (Le) antigenic profile of a variety of fixed tissues. Representative sections of normal and malignant tissue from the stomach, colon, pancreas, and kidney were examined and compared. Lea antigen was expressed more often in gastric adenocarcinomas (80%) than normal gastric mucosa (40%). In gastric tumors with concomitant expression of Le antigens, larger areas within an individual tumor expressed Lea antigen rather than Leb antigen. Expression of Leb antigen in normal colonic tissue was seen only in the proximal colon and not distal colon; colon carcinomas, on the other hand, expressed Leb antigen (64%) regardless of where the primary arose. Leb antigen was expressed in large pancreatic ducts (three of three) more often than Lea antigen (one of three); exclusive expression of Lea antigen was demonstrated in the proximal convoluted tubules of normal kidney (three of three) and in renal cell carcinomas (six of eight).

Animals↗
PubMed1981

Failure of in vitro induction of killer cells in C57BL/6 anti-C57BL/6-LYT1.1 strain combination.

Several in vivo preimmunization schedules which are commonly used for induction of in vitro cell-mediated cytotoxicity against weak transplantation antigens have been tested for ability to generate killer cells in congenic anti B6-Lyt1.1 strain combination. Under the conditions studied no cytotoxic cells were generated. Under the same same conditions good cytotoxic response against H-Y antigen was observed.

Animals↗
PubMed1980

Lyt-1.1 antisera contain antibody against new cell surface alloantigen having wide tissue distribution.

Lyt-1.1 antisera produced in Lyt-1 congenic strain combination are contaminated with additional antibody detecting new cell surface alloantigen showing wide tissue distribution. The antibody was detected by complement independent inhibition of colony forming hemopoietic stem cells. Selective absorption experiments and differences in strain distribution prove that the discovered antigen is different from Lyt-1.

Animals↗
PubMed1979

Identification of TL+ thymocytes with different Lyt phenotypes.

The surface antigenic phenotype of BALB/c thymocytes has been analyzed by sequential cytotoxic treatment with Lyt-1.2, Lyt-2.2 and TL.2 antisera plus complement. Two new minor subpopulations with TL+Lyt-1+2- and TL+Lyt-1-2+ phenotypes have been identified providing direct evidence for the heterogeneity of the Lyt phenotype among the TL-positive supopulation of thymocytes. This finding suggests that differentiation pathways of Lyt subsets of T cells are more complex than originally proposed and that commitment to separate lines of differentiation may occur already at the level of immature, cortical thymocytes.

Animals↗
PubMed1978

Studies on cell surface antigens of mouse leukemic and normal lymphocytes. IV. Functional studies on mouse T lymphocyte subpopulations. B. Lyt phenotype of cytotoxic T lymphocytes and their precursors in B6-Lyt-1.1 mice.

Lyt phenotype of in vitro generated alloreactive cytotoxic T lymphocytes (CTL) and their precursors in B6-Lyt-1.1 (Thy-1.2, Lyt-1.1, Lyt-2.2) congenic strain of mice was studied. The generation of CTL in vitro was completely abrogated by pretreatment of responder cells with antisera to Thy-1.2, Lyt-1.1 and Lyt-2.2 antigens and complement. Mixing of anti-Lyt-1.1-pretreated cells with anti-Lyt-2.2-pretreated cells did not restore CTL generation indicating that both Lyt antigens are expressed on the same precursor cell population. The effect of anti-Lyt sera was specific because the generation of CTL was prevented only when cells from strains possessing appropriate Lyt alleles were pretreated. CTL were eliminated by lysis with anti-Thy-1.2 serum and strongly reduced by anti-Lyt-1.1 and anti-Lyt. 2.2 sera. Nevertheless, a small but significant proportion of CTL was insensitive to lysis with anti-Lyt sera. These data indicate that a phenotype of precursor cells and of majority of CTL is Thy-1.2+ Lyt-1.1+ Lyt-2.2+.

Animals↗
PubMed1978

Studies on cell surface antigens of mouse leukemic and normal lymphocytes. III. Preparation and characteristics of alloantisera to differentiation antigens of mouse T lymphocytes.

Preparation of antisera to alloantigens Thy-1, TL, Lyt-1 and Lyt-2 is described and the importance of adequate specificity controls indicated. The specificity and activity of antisera were characterized by trypan blue exclusion cytotoxicity assay, indirect membrane immunofluorescence and absorption using cell populations from different tissues of Thy-1, TL and Lyt congenic strains of mice. In the course of these studies new serologically detectable Lyt-1 linked alloantigen was discovered which shows wide tissue distribution.

Animals↗
PubMed1977

Production of antibodies against ML antigen in the xenogeneic host.

By using various protocols of immunization in the xenogeneic system, a serum was produced which contained antibodies against normal as well as leukemic lymphocytes in DBA/2 mice. To obtain specific antibodies capable of recognizing the ML tumor antigen, the best protocol proved to be the one used in group 2, in which immunization with leukemic cells was preceded by immunization of newborn mice with normal lymphoid cells from mice of the DBA/2 f BALB/c/MTV-s-negative strain. The sera contained antibodies capable of identifying the ML antigen in leukemia cells as well as in isolated form.

Animals↗