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Biomedical subjects

M Yaginuma

Publications and source records attributed to M Yaginuma.

At least 19 recordsLinked to original sources
PubMed2001

5-Bromodeoxyuridine suppresses position effect variegation of transgenes in HeLa cells.

An ectopic gene integrated in the host genome is occasionally silenced due to a position effect of its adjacent chromatin structure. We found that 5-bromodeoxyuridine clearly activated such a transgene in HeLa cells. The transgene was also activated to various degrees by inhibitors of histone deacetylase, DNA topoisomerases, or DNA methyltransferase. The peptide antibiotic distamycin A potentiated markedly the effect of 5-bromodeoxyuridine. Transient expression of an artificial AT-hook protein termed MATH20 also potentiated its effect although significantly activated the transgene alone. Since distamycin A and MATH20 are able to displace histone H1 and other DNA-binding proteins bound to specific AT-rich sequences by a dominant, mutually exclusive fashion, these results suggest that 5-bromodeoxyuridine targets such an AT-rich sequence located adjacent to the silenced transgene, resulting in chromatin accessibility.

Antimetabolites, Antineoplastic↗
PubMed2001

Atypical form of dural graft associated Creutzfeldt-Jakob disease: report of a postmortem case with review of the literature.

A postmortem case of an atypical form of dural graft associated Creutzfeldt-Jakob disease (CJD) is described. A 42 year old man developed progressive spastic paresis 163 months after a cadaveric dura mater graft. He presented with no myoclonus and very late occurrence of periodic synchronous discharges on EEG. The prion protein (PrP) gene was homozygous for methionine at the polymorphic codon 129. Neuropathological examination disclosed plaque-like PrP deposits with atypical distribution of synaptic PrP accumulations in the brain. This patient represents an atypical form of dural graft associated CJD characterised by unusual clinicopathological features.

Adult↗
PubMed2000

Acute cerebellar ataxia of a patient with SLE.

We described a 28-year-old woman with systemic lupus erythematosus (SLE) presented with digestive tract, skin and renal symptoms and afterwards developed acute cerebellar ataxia, a paresis of the right inferior rectus muscle, left abducens paralysis and left facial palsy which seemed to be consistent with a brainstem lesion visible on magnetic resonance imaging (MRI). This lesion disappeared within 9 days of corticosteroid treatment. It is suggested that this lesion is focal edema induced by acute changes in the blood brain barrier secondary to a vasculopathy. Other causes, including local infarction, are unlikely.

Acute Disease↗
PubMed2000

Connection designs of three different implant systems in the resorbed edentulous maxilla: a case report.

This report presents the mixed use of three different system implants for an implant-supported fixed bridge in a resorbed maxilla. Two of six implants that had been placed were lost. New implants were combined with the remaining implants that had been placed by the previous dentists in 1992 and 1997. The three implant systems consisted of the following: one incorporated an intramobile element into an implant device, and the other two were whole titanium screw-type implants (one with a machined surface and the other with a plasma-sprayed surface). This clinical report describes the connection designs of these different system implants to the fixed bridge and lists the complications that followed.

Bone Resorption↗
PubMed2000

[A case of hemolytic uremic syndrome caused by verotoxin producing enteropathogenic Escherichia coli (O157:H7) with prolonged cerebrovascular dysfunction].

A 56-year-old woman was admitted to our hospital because of bloody watery diarrhea. Stool cultures on admission revealed the verotoxin producing enteropathogenic Escherichia coli (VTEC) (O157:H7). Hemolytic uremic syndrome (HUS) appeared on the 4th hospital day. Plasma exchange was performed from the 4th day to the 11th day. Mental confusion appeared on the 6th day, and was aggravated until the 18th day, and slowly improved afterwards. Serial cranial CT in series demonstrated low density areas in the bilateral parietooccipital lobes on the 26th day, and the left one diminished but the right one became more evident on the 49th day. The series of single photon emission computed topography (SPECT) revealed that in the region of the temporoparietooccipital lobe cerebral blood flow decreased on the left side on the 30th day, on both sides on the 39th day, and on the right side on the 82nd day. There are few reports of prolonged cerebrovascular dysfunction observed in serial studies of SPECT in a patient of HUS by VTEC (O157:H7). We should be aware, however, of the appearance of the CNS involvement in the recovery stage because the prolonged cerebrovascular dysfunction may occur as our case.

Cerebrovascular Circulation↗
PubMed1999

Case report: acute encephalitis immediately prior to acute onset of hepatitis C virus infection.

A 34-year-old male presented with acute viral encephalitis of unknown aetiology with subsequent acute onset of hepatitis C virus (HCV) infection. Although the neurological syndrome improved after administration of acyclovir, jaundice appeared. Neurological complications of HCV infections have rarely been described. In this case report, we discuss the close relationship between neurological syndromes and HCV infection. In the future, we hope that further discussion of clinical cases will determine whether or not the HCV produces neurological manifestations.

Acute Disease↗
PubMed1998

Quality evaluation of radiographic contrast media in large-volume prefilled syringes and vials.

RATIONALE AND OBJECTIVES: The authors compared the particle contaminations of radiographic contrast media packaged in large-volume prefilled syringes and vials. MATERIALS AND METHODS: Particle counting was performed for four contrast media packaged in large-volume prefilled syringes (iohexol, ioversol, ioversol for angiography, and ioxaglate) and three contrast media packaged in vials (iohexol, ioversol, and ioxaglate). X-ray emission spectrometry was performed to characterize the individual particles. The amount of silicone oil in the syringe was quantified with infrared spectrophotometry. RESULTS: The particle contamination in syringes containing ioversol was higher than that in syringes containing iohexol or ioxaglate. Particle contamination in the vials was relatively low, except with ioxaglate. X-ray emission spectrometry of the components of the syringe and vial showed that the source of particles was internal material released from the rubber stopper or inner surface. CONCLUSION: The particle counts for contrast media packaged in syringes and vials varied considerably among the different contrast media and were related to the amount of silicone oil on the inner surface and rubber piston of the syringe.

Contrast Media↗
PubMed1997

[A case of repetitive cerebellar ataxia with persistent primitive trigeminal artery].

We reported a case of 40 year-old-male, with the persistent primitive trigeminal artery (PPTA), who had exhibited transient dysarthria and gait disturbance since he was 30 years old. The symptoms gradually became aggravated in both frequency and duration. The results of the angiography showed that hypogenesis of bilateral vertebral artery and PPTA originated from the left internal carotid artery and joined to the upper portion of the basilar artery. Single photon emission computed tomography (SPECT) revealed no abnormality, whereas those of MRI showed PPTA compressing the left ventral part of the pons. It seemed probable that transient cerebellar ataxia occurred with transient compression of the ventral part of the pons, containing the pontocerebellar fibers, by the expansion of the lumen of PPTA.

Adult↗
PubMed1996

Development of protamine-bonded phase for separation of saccharides in liquid chromatography.

A protamine-bonded polymer gel was synthesized by passing this protein through an activated carbamate polyamine polymer gel layer. This new packing material was suitable for versatile separation of monosaccharides, oligosaccharides, sugar alcohols and uronic acids in liquid chromatography. The column packed with this gel could be operated at room temperature and gave excellent recovery for the reducing monosaccharides such as 2-deoxyribose, 2-deoxyglucose, ribose and mannose for which the conventional alkylamine-bonded phase column gave poor recovery. This column was used for the analysis of saccharides in urine using a post-column reaction detection system.

Amino Acid Sequence↗
PubMed1995

[A case of Sjögren's syndrome with rheumatoid arthritis manifesting transverse myelitis with antineuronal antibody].

We report a sixty-year-old woman with transverse myelitis who had suffered from rheumatoid arthritis since age of 52. She was admitted to our department because of muscle weakness and painful tonic spasm in the bilateral lower extremities, sensory disturbance below the mamillary level and bladder disturbance. She had sicca symptoms. As a result of sialography, Sjögren's syndrome was diagnosed. Antineuronal antibody was found in the sera of the patient. She had no symptom of systemic vasculitis. Lupus anticoagulant and anticaldiolipin antibody were negative. The pathogenesis of transverse myelitis in rheumatic disease is still uncertain. Vasculitis and the immunological reaction of antineuronal antibody have been suggested as possible causes. This report suggests the influence of direct immunological reaction on the central nervous system.

Arthritis, Rheumatoid↗
PubMed1992

Effect of haloperidol on cyclic AMP and inositol trisphosphate in rat striatum in vivo.

To investigate the effect of haloperidol (HAL) on second messengers in the brain striatum, the concentrations of cAMP and inositol trisphosphate (IP-3) were measured in the striatum of rats in vivo after intravenous administration of HAL, and their concentrations were compared with the severity of catalepsy and changes in dopamine (DA) metabolism in the striatum. Catalepsy developed both in the animals treated with 5 mg/kg and those with 0.5 mg/kg of HAL, but it appeared earlier, and the period of severe catalepsy was longer in the former than in the latter. In the animals treated with 5 mg/kg of HAL, DOPAC and HVA began to increase at 20 min after administration, and their percent increases were correlated with the severity of catalepsy. In the 5 mg/kg animals, both cAMP and IP-3 increased. The IP-3 showed a delayed peak but a greater increase as compared with the cAMP. In the 0.5 mg/kg animals, only IP-3 increased. These findings suggest that HAL might affect not only the adenylate cyclase system but also the phosphoinositide response in the striatum. Moreover, the changes in the phosphoinositide response might be secondarily induced by the blocking of D-2 receptors by HAL.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1990

Improvement of unilateral spatial neglect with numbering.

We examined 8 patients with moderate to severe left unilateral spatial neglect by means of a series of line cancellation tasks. We asked the patients to cross out lines in the 1st trial, to number lines in the 2nd trial, and again to cross out lines in the 3rd trial. In the 2nd trial with numbering, all the patients showed improvement of left unilateral spatial neglect. Numbering involved the successive use of increasing numbers. This process seemed to motivate the patients to continue searching for another line, even at the point when they would be satisfied that they had completed the task if they used simple crossing-out. Insufficient motivation for visuospatial searching may play an important role in unilateral spatial neglect observed in cancellation tasks.

Aged↗