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Biomedical subjects

M Z Saidov

Publications and source records attributed to M Z Saidov.

16 recordsLinked to original sources
PubMed1993

Peripheral blood neutrophils from HIV-1-infected individuals are armed with factors that cause inhibition of their migration in response to specific antigens.

In this study, synthetic peptides that copy conserved regions of the HIV-1 envelope proteins gp120 and gp41 were tested for their impact on the chemotaxis of leukocytes and neutrophils from HIV-1-infected individuals, while neutrophils from HIV-1-infected patients were tested for their effect on the chemotaxis of neutrophils from healthy donors. The synthetic peptides (corresponding to the 251-272-amino acid sequence of gp120 and the 584-618-amino acid sequence of gp41) were capable of specifically inhibiting the formyl peptide-induced chemotaxis of cells from HIV-1-infected patients, and such inhibition was observed both with a total leukocyte population and with pure neutrophils. The migration of neutrophils from healthy donors was specifically inhibited in the presence of either of the synthetic peptides of HIV-1 envelope proteins after their incubation with neutrophil supernatants obtained from HIV-1-infected individuals. As shown by ELISA tests, the neutrophil supernatants from HIV-1-infected individuals contain antibodies to a recombinant env-1 protein that might be one of the reasons for the specific arming of neutrophils from HIV-1-infected persons.

Adult↗
PubMed1993

Peripheral blood leukocytes from multiple sclerosis patients are coated with factors inhibiting their chemotaxis in the presence of myelin basic protein.

In this study myelin basic protein (MBP) was tested for its effect on chemotaxis of human peripheral blood leukocytes (PBL) and neutrophils from multiple sclerosis (MS) patients. MBP appeared to inhibit specifically the formyl-methionyl-leucyl-phenylalanine (FMLP)-induced chemotaxis of both the total leukocyte population and neutrophils. In comparison, no inhibition of chemotaxis was observed in healthy donors and patients with other neurological diseases. From MS patients we collected neutrophil supernatants obtained by incubation of the cells in a serum-free medium at 37 degrees C for 60 min and evaluated their effect on chemotaxis of neutrophils from healthy donors. Chemotaxis of healthy donor neutrophils was inhibited specifically in the presence of MBP after treatment with these supernatants, presumably relating to the presence of immune complexes on the surface of neutrophils from MS patients. Those complexes can be eluted into the incubation medium and coat healthy donor neutrophils, thus arming them specifically.

Adult↗
PubMed1992

Mononuclear cells from HIV-infected patients produce factors which enhance functional activity of polymorphonuclear neutrophils from healthy subjects.

The influence of mononuclear cell supernatants (MNCS) from nine healthy donors and 35 HIV-infected patients (17 with lymphoadenopathy syndrome (LAS), 15 with ARC and three with AIDS) on functional activity of polymorphonuclear neutrophils (PMN) from healthy donors was investigated. MNC after short-term cultivation (24 h) produced factors which enhanced chemiluminescence (CL) and chemotaxis of PMN. This augmentation did not depend on stimulation of MNC by mitogens (lipopolysaccharide Escherichia coli (LPS) and concanavalin A (Con A)) or on activation of PMN by FMLP. After 48 h of cultivation only MNC stimulated by LPS produced these factors. MNCS from HIV-infected patients provoked a more pronounced augmentation of PMN CL compared with MNCS from healthy subjects. This enhancement was observed in patients at all stages of infection, but was more pronounced in patients with LAS. MNCS impact on PMN CL was not connected with proliferative activity of MNC but was correlated with the level of CD4 cells. It was shown that removal of adherent cells from MNC fraction resulted in decreased MNCS impact. Treatment of MNCS by antibody to IL-1 beta, IL-8, interferon-alpha (IFN-alpha) and tumour necrosis factor-alpha (TNF-alpha) did not decrease MNCS impact on PMN CL.

AIDS-Related Complex↗
PubMed1992

[Comparative analysis of functional activity of peripheral blood phagocytes in HIV-infected patients and in those with recurrent herpes simplex].

The functional activity of peripheral phagocytes were comparatively studied in 14 HIV-infected patients and 28 patients with chronic Herpes simplex viral infection. The two groups exhibited lowered adhesive capacity of phagocytes, impaired production and excretion of active oxygen metabolites. In addition, the patients with chronic Herpes simplex infection showed much elevated levels of myeloperoxidase and acid phosphatase, which indicated its compensatory pattern. The HIV infected had no enhanced enzymatic activity. One cannot rule out that these differences in the functional activity of phagocytes are associated with different effects of viral peptides on the cellular wall of phagocytes.

Acid Phosphatase↗
PubMed1991

[A comprehensive study of the phagocytic link in immunity in primary immunodeficiency states].

The results of the study of the phagocytic element of immunity in cases of primary immunodeficiency, made with the use of a complex of functional tests, are presented. As shown in this study, in patients with definitely characterized phagocytic defect (chronic granulomatous disease) a decrease in all parameters under study, with the exception of phagocyte index, is observed. In patients with immunodeficient states of nonphagocytic nature moderate mosaic deviations of phagocytosis characteristics are noted. For further and more profound study of phagocytosis disturbances in such patients, it is expedient, in particular, to carry out studies in the activity of myeloperoxidaze and the intensity of oxygen-dependent metabolism. The use of the complex of methods used in this investigation has shown their high diagnostic value. It may be recommended for clinical practice under the condition of strict standardization of experimental techniques.

Adolescent↗
PubMed1991

[A spectrophotometric method of determining the myeloperoxidase activity in phagocytic cells].

A spectrophotometric method has been developed for measurements of myeloperoxidase activity in phagocytes, and conditions of measurements specified. Contribution of mononuclear cells to myeloperoxidase activity was found negligible, the major role here was played by neutrophils and eosinophils. Myeloperoxidase activity was found reduced in the patients with chronic granulomatous disease, agammaglobulinemia, and elevated in hyper-IgE-syndrome; this parameter was unchanged in ataxia telangiectasia and chronic dermatomucosal candidiasis.

Agammaglobulinemia↗
PubMed1991

[Cellular immunity function in HIV-1-infected persons].

The data on the state of cell-mediated immunity in patients with AIDS-related complex are presented. The synthetic peptide of membrane protein gp120 of HIV-1 was shown to inhibit leukocyte adhesion in persons under examination, as well as to have the tendency towards inhibiting the chemotaxis of migratory cells. The maximum effect was achieved at a peptide concentration of 10(-6) M. The data obtained in this investigation suggest the presence of specific cell-mediated sensitization to the fragment of protein gp120, detected by the adhesion inhibition test with the use of spectrophotometric techniques and the capillary evaluation of the chemotaxis of migrating cells, in patients with AIDS-related complex.

AIDS-Related Complex↗
PubMed1991

[Inhibitor factors in the serum of patients with the AIDS-related complex].

The data on the presence of factors blocking the reaction of E-rosette formation and leukocyte chemotaxis in the blood sera of patients with AIDS-related complex (ARC) and HIV-positive donors are presented. Most frequently the blocking of E-rosette formation coincided with the presence of a inhibiting effect on the migration capacity of leukocytes. This blocking activity was not linked with the presence of C-reactive protein in the circulation stream. The treatment of ARC patients with plasmapheresis and/or travolol was accompanied either by the disappearance of blocking activity or by the appearance of activity stimulating E-rosette formation.

AIDS-Related Complex↗
PubMed1986

[Action of T-activin on human natural killer activity in vitro].

The activity of natural killers (NK) from human peripheral blood was determined by 3H-uridine test using target cells K-562. T-activin effect on the activity of human NK in vitro depended on two parameters: the preparation dose and effector/target cells ratio. The inhibitory effect of T-activin was observed with high doses and increased E/T ratio, while the activating effect was noted with low doses and reduced E/T ratio. This can be attributed to the heterogeneity of NK population, different functional role of high and low doses of thymic factors and the development of NK as T-cell precursors.

Adjuvants, Immunologic↗
PubMed1985

[Quantitative and functional evaluation of immunoregulating cells and natural killers in patients with aplastic anemia].

Cell-mediated immunity was studied in 11 patients with idiopathic aplastic anemia according to several parameters at a time. Analysis of the data obtained draws attention to the pronounced relative lymphocytosis, disordered balance of the immunoregulatory subpopulations of T lymphocytes (T mu and T gamma) associated with an appreciable increase in the number of cytotoxic T suppressor cells. The lowering of spontaneous and optimal Con A dose-induced production of leukocyte migration inhibition factor and deficiency of the activity of normal killers can be determined by potentiation of the suppressor activity of T lymphocytes or by the influence of factors produced by T suppressors. The goal-oriented correction of the high suppressor activity of T cells may appear promising in the complex of therapeutic measures used in the management of the patients' population with idiopathic aplastic anemia.

Anemia, Aplastic↗
PubMed1984

[Antibody-dependent cell cytotoxicity and the effect of interferon on K-cell functional activity in vitro in chronic viral hepatitis B in children].

Antibody-dependent cell-mediated cytotoxicity (ADCC) and in-vitro effect of interferon (IF) on the K cell activity was investigated in children with chronic hepatitis B (CHB) and normal donors. The ADCC in CHB was significantly decreased under normal values. The low K cell activity in CHB might determine inadequate elimination of infected liver cells and continuation of hepatitis B virus replication. IF in vitro significantly stimulated the ADCC of normal blood donors but was unable to significantly increase the ADCC of CHB patients. The in-vitro stimulation of the ADCC of normal donors by IF might be a phenomenon due to an increase of the adult K cell cytotoxic potential and/or activation of pre-K cell maturation. The lack of in-vitro augmentation of the ADCC of CHB patients by IF might result from the decreased quantity of K cells of pre-K cells in the peripheral blood and/or their low susceptibility to the stimulating effect of IF.

Adolescent↗
PubMed1984

[Change in the activity of natural killer cells in normal subjects and in virus diseases on exposure to interferon in vitro].

The activity of natural killers was examined in peripheral blood of healthy subjects and patients with chronic hepatitis and disseminated sclerosis. An attempt was made to correct natural killer activity by human leukocyte interferon in vitro. To assess the activity of natural killers, use was made of the method of serial dilutions. An optimal effector/target ratio was employed in experiments. The patients with chronic hepatitis and disseminated sclerosis demonstrated a reduction in the activity of natural killers whatever the effector/target ratio. The action of interferon in vitro is specific immunomodulatory in nature. Administration of interferon in a dose of 250 Units/ml raises the magnitude of the cytotoxic index in healthy donors and in patients with chronic hepatitis and disseminated sclerosis, making the shape of the killer activity curve approach that of normal. Such an approach can be used for preliminary assessment of the sensitivity of natural killers to interferon in viral diseases of man. The potentialities and efficacy of interferon in clinical medicine are discussed.

Hepatitis B↗
PubMed1983

[Role of immunoregulatory cells and natural killers in the pathogenesis of multiple sclerosis].

Simultaneous investigations of the immunoregulatory cells and the natural killers were carried out in patients with different stages of multiple sclerosis in an attempt to determine a relationship between these organisms and the clinical stage of the disease. The quantities of E- and EAC-RFC in patients of all groups were within normal. The amount of T gamma-cells was decreased in the progressive phase of the disease and was significantly higher than normal during remission. The activity of the natural killers in the progressive phase of disease did not differ from control but was significantly depressed during remission with all effector/target ratios. The activity of the natural killers and the amount of T gamma-cells were found to be reversely correlated both in the progressive and remission phases of the disease. A quantitative reduction in the 0 population of lymphocytes was accompanied by a sharp suppression of natural killer activity.

Adolescent↗