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M Zapata

Publications and source records attributed to M Zapata.

30 records · Page 2Linked to original sources
PubMedDate not supplied

[Production and characterization of monoclonal antibodies directed agains the major outer membrane protein and lipopolysaccharide of Chlamydia trachomatis].

Two monoclonal antibodies (MnAb) directed against the lipopolysaccharide (LPS) and major outer membrane protein (MOMP) of Chlamydia trachomatis were produced for use in indirect immunofluorescence (IFI). The specificity of the antibodies was determined by Dot-blot, Immunoblotting (IB) and IFI onto culture cells infected with C. trachomatis and IFI onto commercial swabs (MRL). The MnAb 2D3 and 3C2 detected LPS and MOMP of C. trachomatis, respectively, by different methods. Neither MnAb showed cross-reactions when other gram-negative bacteria were used as antigens.

Animals↗
PubMedDate not supplied

Detection of rubella-virus-induced apoptosis in Vero cell cultures with hematoxylin and eosin staining.

In order to facilitate the detection of apoptotic cells (Apo C) in Rubella virus (RV) infected cultures in settings of low resources, we compared hematoxylin and eosin staining (H&E) with the conventional TUNEL technique, and confirmed our findings with DNA electrophoresis and transmission electron microscopy. H&E allowed to distinguish Apo C from non-apoptotic cells. The proportion of Apo C in infected cultures was proportional to the multiplicity of infection (MOI). At a MOI of 10, the percent of Apo C at 3, 4 and 5 days post infection (pi) were 26, 45 and 47%, respectively, which were significantly reduced when the caspase inhibitor z-VAD-fmk was present in the supernatant. By the TUNEL assay, the percent of Apo C in RV-infected cultures were lower (0.8, 1.2 and 1.2% at 3, 4 and 5 days pi, respectively). Our results have shown that H&E staining is an easy, rapid, economic and reproducible method to detect Apo C in RV infected Vero cells cultures. It is possible that H&E makes evident early stages of apoptosis, when an apoptotic cell shows chromatin condensation, nuclear and cytoplasmic contraction (but is still attached to the monolayer), while TUNEL detects later stages of apoptosis because it needs an extensive DNA fragmentation, when apoptotic cells are about to or have already detached from the substratum.

Amino Acid Chloromethyl Ketones↗
PubMedDate not supplied

[Perinatal factors associated with attention deficit/hyperactivity diagnosis in Colombian Paisa children].

INTRODUCTION: Some perinatal factors have been associated with attention d ficit/hyperactivity disorder (ADHD). OBJECTIVE: To estimate the association between perinatal factor and ADHD diagnosis in school, aged 6 to 11 years, children from Medell n city (Colombia). PATIENTS AND METHODS: A randomized sample of 200 cases, 6 to 11 year old, with standardized ADHD diagnosis was selected. A randomized sample of 200 control children, 6 to 11 year old, was selected from a data base of 70 schools of Medell n city. The same diagnostic protocol was administered to controls. A questionnaire with questions about pregnancy, delivery an neonatal period was given to the mothers. Data were analyzed using cross tabulation and stepwise logistic multiple regression analyses. RESULTS: Miscarriage symptoms, premature delivery symptoms, severe flu attacks (cold with fever, without virus confirmation), tobacco abuse, alcohol abuse (to become drunk), minor congenital malformations, neonatal seizures and hospitalizations during newborn period were factors associated with ADHD diagnosis (p< 0.05). However, stepwise logistic multiple regression analysis selected a block of variables formed by premature delivery symptoms, severe flu attacks and neonatal seizures as significant perinatal risk factors (p< 0.01) for ADHD diagnosis.

Attention Deficit Disorder with Hyperactivity↗
PubMedDate not supplied

[Isolation of Chlamydia trachomatis in populations with different risks of infection].

The level of infection by Chlamydia trachomatis in patients attending different units of urogenital diseases was evaluated. One hundred and fifty four patients divided in four groups were studied. Fifty percent of C. trachomatis isolation was achieved in the high risk group A, composed by men attending to a sexual transmission disease service. Among men attending an urology unit (group B) isolation reached 20.7%. In group C composed by women from a general gynecological unit the rate of isolation was 13.3%; and in group D (men and women with infertility problems) the rate of isolation was 4.3%. These isolation values were achieved combining the primoinoculation of cell cultures with a posterior blind passage. The known risk factors such as the number of sexual partners, age and previous data of sexually transmitted diseases affected the percentages of C. trachomatis isolation from each group.

Adolescent↗
PubMedDate not supplied

[Effect of the type of swab on the isolation of Chlamydia trachomatis].

The success in the isolation of C. trachomatis is dependent on several factors. One of them is the type of swab used for collection the specimens. As some types of swabs, such as calcium alginate and aluminum, cotton and wood have recently been shown to decrease the isolation rate, different types of swabs were prepared and tested in our laboratory. Commercial aluminum, type 5056 aluminum and stainless steel were used with the same type of cotton. Commercial swabs of cotton and wood were tested too. The swabs were incubated with 1 ml of transport medium and a known number of UFI for 24 h at 4 degrees C, then were processed for isolation. The isolation rate with commercial aluminum was 95%, with aluminum 5056 was 91% and with stainless steel was 98%. The swabs made of cotton and wood were toxic to McCoy cells, destroyed the monolayer and did not allow the recovery of C. trachomatis. The poor recovery of Chlamydia from some types of swab, indicates that the available swabs used in each laboratory should be periodically evaluated to avoid false negative results.

Chlamydia Infections↗
PubMedDate not supplied

[Use of a rapid method for determining primary infection by rubella virus].

The assay of specific immunoglobulin M (IgM) antibodies could aid in the diagnosis of early viral infection. Here we described the results obtained for IgG and IgM separation on a small column of Sephadex A-50 ion exchanger and the subsequent measure of hemagglutination inhibition antibodies for rubella virus. This diagnostic procedure combines the efficiency, rapidity and simplicity of a chromatography method with the detection of specific antibodies by reference method. The average immunoglobulin recuperation was 91% for the immunoglobulin G (IgG) and 70% for the IgM. Eighteen sera obtained from patients with presumptive diagnostic compatible with primary rubella infection, 10 from patients with known immune status for rubella virus, and 3 sera with specific IgM for BK virus, were comparatively studied by gel filtration. Sephadex G-200, enzyme immunoassay for rubella IgM antibody and Sephadex A-50 ion exchanger. The results indicated that the proposed method can be satisfactorily used for the diagnostic of primary rubella virus infection.

Antibodies, Viral↗