Biomedical subjects

M Zawadzka

Publications and source records attributed to M Zawadzka.

8 recordsLinked to original sources

PubMed1995

Visualization of accessory nuclei from oocytes of the sawfly Athalia rosae (Hymenoptera: Tenthredinidae) by a spreading technique.

Oocytes of hymenopterans are equipped with specific organelles termed accessory nuclei (AN). These organelles are surrounded by a double envelope and contain RNA-positive inclusions. We have employed the spreading technique to analyse AN isolated from the ooplasm of the sawfly Athalia rosae. In electron microscopic spreading preparations AN appear as membranous, extensively folded structures. In their vicinity, numerous nuclear pore complexes have been found. This observation confirms the presence of pores in the envelopes of accessory nuclei.

Animals↗

PubMed1993

Genotoxic effect of triarylmethane dye--acid green 16 after chronic ethanol consumption in mice.

Genotoxic effect and hepatic microsomal monooxygenase activities were assessed in mice treated with Acid Green 16 (single i.p. injection at dose 75 mg/kg) superimposed on prolonged ethanol consumption (10% solution in drinking water for 2-4 months). Treatment of mice with Acid Green 16 led to an increased frequency of micronucleated erythrocytes in bone marrow. In animals pretreated with ethanol the frequency of micronucleated erythrocytes, produced by Acid Green 16, was significantly higher. The changes in frequency of micronucleated erythrocytes were accompanied by the enhanced activity of microsomal monooxygenases manifested by higher activity of 7-ethoxycoumarin o-deethylase (the level of cytochrome P-450 was not altered). The obtained results showed that ethanol tended to increase the genotoxic effect of Acid Green 16. However, the slight inductive effect of ethanol on microsomal monooxygenases did not provide clear evidence that the genotoxic effect of Acid Green 16 was associated with ethanol stimulation of the metabolic activation of the dye in the liver.

Animals↗

PubMed1978