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Biomedical subjects

M Zerbini

Publications and source records attributed to M Zerbini.

104 records · Page 6Linked to original sources
PubMed1984

Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

An enzyme linked immunosorbent assay (ELISA) for detecting antibodies against cytomegalovirus induced immediate early antigens and early antigens was developed using purified nuclear antigens and was compared with the indirect immunofluorescence test. The tests were comparable in their ability to detect positive and negative sera, and antibody titres determined by both assays were similar. The use of ELISA for the detection of antibodies against cytomegalovirus induced immediate early and early antigens is advocated in diagnostic and research laboratories.

Antibodies, Viral↗
PubMed1983

Can Epstein-Barr virus infect and transform all the B-lymphocytes of human cord blood?

Quantitative aspects of Epstein-Barr virus infection and transformation of human neonatal B-lymphocytes have been investigated. 72 to 90% B-cells were obtained with enrichment. Of the B-cells, 19 to 97% showed nuclear antigen (EBNA) 2 days after infection. A difference between different B-cell donors in susceptibility to infection was noted. Analysis of the virus dose-response curves obtained with twofold virus dilutions showed that one virus particle is sufficient to induce EBNA in a cell. Of the infected cells, 50 to 95% multiplied in microtitre wells containing a human fibroblast feeder layer, while only a small proportion established growing colonies in soft agarose, that could be picked up and subcultured.

Animals↗
PubMed1982

Serum antibody profile against herpesviruses in renal transplant recipients.

Forty-eight renal allograft recipients who had received a kidney graft 2 months to 8 years previously, were followed for the presence of serum antibody against Cytomegalovirus (CMV), Epstein-Barr virus (EBV), Herpes simplex virus (HSV), and Varicella-Zoster virus (VZV). These investigations were performed over a 6 months period and in at least two serum samples from each patient, obtained at intervals of 2-3 months. The presence of serological signs of recent or active infection were observed in 31 patients for CMV, in 22 patients for EBV (18 patients were positive both for CMV and EBV) and only in 3 patients for HSV, while no patients showed serological signs of recent or active infection by VZV.

Adolescent↗
PubMed1979

Inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells.

The study of human cytomegalovirus (HCMV) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin D, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. In the same experimental conditions the growth of poliovirus type I, vaccinia virus and herpes simplex type I virus, was completely unaffected. These results suggest that the complete HCMV replication cycle requires some cellular function(s) between early transcription of the input virus genome and virus DNA synthesis.

Antigens, Viral↗
PubMedDate not supplied

Localization of polypeptides in frog virus 3 by radioiodination technique.

Frog virus 3 is an amphibian icosahedral deoxyribovirus which replicates in the cytoplasm of infected cells. The radioiodination of purified virions using the enzyme lactoperoxidase has shown that the major component of the structural proteins is only partially exposed through the lipoprotein membrane that constitutes the outer layer of the icosahedral capsid.

Animals↗
PubMedDate not supplied

[Characteristics of various NAG vibriones isolated in Italy].

In this paper are reported the results of a comparative study of 4 "NAG" vibrio strains isolated in Italy and the vibrio biotype "E1 Tor" responsible of the 1973 outbreak. The "NAG" vibrios were practically identical with the "E1 Tor" vibrio in biochemical properties, polypeptide composition, enteritogenic activity in rabbit ileal loops and showed various antigenic similarities in gel precipitation and indirect immunofluorescence tests. The possible pathogenic role of the "NAG" vibrios is briefly discussed.

Agglutination Tests↗