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Biomedical subjects

M Ziff

Publications and source records attributed to M Ziff.

At least 199 records · Page 11Linked to original sources
PubMedDate not supplied

Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus.

A study of the immunoglobulin (Ig) synthesis by peripheral blood cells of patients with systemic lupus erythematosus (SLE), rheumatoid arthritis, and controls was carried out. Synthesis was markedly elevated in patients with active SLE, whereas patients with the active SLE or RA showed normal values. Treatment of 3 patients with active SLE resulted in a reduction of Ig synthesis that correlated well with the disappearance of disease activity. There results indicate that the abnormal immune process in active SLE, characterized by intense B-lymphocyte stimulation, is associated with the appearance of more highly differentiated B cells in the blood.

Antibodies, Antinuclear↗
PubMedDate not supplied

Lymphokines in the rheumatoid joint.

Joint fluids and culture supernatants of synovial tissue were examined for the presence of two lymphokines. Migration inhibitory activity was found in 16 of 22 rheumatoid fluids (73%), in 3 of 15 osteoarthritis fluids (20%), and in 3 of 11 fluids from patients with various inflammatory arthritides (27%). Blastogenic activity was present in 14 of 15 rheumatoid fluids and in 1 of 7 nonrheumatoid effusions. The active materials eluted with the third peak from Sephadex G-200 and were therefore smaller than immunoglobulin or immune complexes. These findings suggest that lymphokine-like substances were present in effusions and synovial tissue of most patients with rheumatoid arthritis and some patients with other forms of chronic synovitis.

Arthritis, Reactive↗
PubMedDate not supplied

Electron microscopic studies of the cartilage-pannus junction in rheumatoid arthritis.

The junction between pannus and cartilage was examined in the rheumatoid joint. Three types of cartilage-pannus junction were observed. In one, proliferating small blood vessels, surrounded by highly cellular infiltrates, penetrated deeply into the cartilage. Degeneration of the cartilage was observed around the cellular accumulations. In the second, phagocytic and fibroblastic cells invaded the cartilage. In the third, fibrous pannus overlay the cartilage. Lysis of cartilage by infiltrating cells appeared to be a major cause of cartilage erosion in rheumatoid arthritis.

Acid Phosphatase↗
PubMedDate not supplied

Identification of immunoglobulins and complement in rheumatoid articular collagenous tissues.

Ninety-three patients with a variety of joint diseases were studied for evidence of immune complexes in articular collagenous tissues. Frozen sections of freshly obtained biopsies of hyaline articular cartilage and menisci were stained with fluoresceinated monospecific antisera for evidence of human immunoglobulins (IgG, IgM,IgA) and the beta1c component of complement. The criterion for the presence of complexes was the staining of two or more immunoglobulins and beta1c in an identical location of sequentially cut sections. Of the 42 patients with rheumatoid arthritis (RA) 83% were positive by this criterion. In those with classic RA the incidence was 92%. Sixteen patients with fresh joint trauma or nonarthritic disease had negative findings. Among 26 patients with noninflammatory disease, 4 of 8 with polyarthritis whose features suggested primary degeneration, 1 of 11 patients with secondary degenerative arthritis, and a single case of synovial osteochondromatosis had positive findings. Among 9 patients with miscellaneous inflammatory arthritides, all of 3 with psoriatic arthritis were negative; however 2 of 6 with other inflammatory arthritides were positive. The findings in classic RA suggest that immune complexes are deposited in the articular collagenous tissues. The persistence of these complexes may play a significant role in the chronicity of the synovitis.

Adolescent↗
PubMedDate not supplied

Electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage.

Horseradish peroxidase (HRPO) conjugated with goat antihuman IgG, goat antihuman IgM, and aggregated human IgG has been used as a enzymatic marker to stain IgG, IgM, and rheumatoid factor in rheumatoid cartilage. When Hrpo-anti IgG and HRPO-anti IgM were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. However 7 of 8 rheumatoid cartilage specimens stained with HRPO-anti IgG showed electron-dense deposits. Three rheumatoid specimens stained with HRPO-anti IgM showed similar findings. Both of 2 rheumatoid specimens also stained positively with HRPO conjugated with aggregated IgG, a finding indicating that rheumatoid factor was present. The deposits were seen between the collagen fibers of the superficial layer of the cartilage to a maximal depth of 22 mu from the surface (average: 7 mu). The amorphous fibrinous material on the surface of the cartilage was also stained. The demonstration of IgG, IgM, and rheumatoid factor in the superficial zone of rheumatoid cartilage suggests that immune complexes are deposited in the cartilage in this disease.

Arthritis, Rheumatoid↗
PubMedDate not supplied

Electron microscopic observations of immunoreactive cells in the rheumatoid synovial membrane.

The perivascular infiltrates of the rheumatoid synovium were examined in the electron microscope. Lymphocyte-rich, plasma cell-rich, and transitional areas were observed. The transitional areas contained lymphocytes, plasma cells, blast cells, macrophages, and fribroblasts. Although lymphoblasts were frequent, the blast cells were predominantly plasmablasts. Marked degeneration of fibroblasts in the vicinity of lymphoblasts suggested the liberation of a lymphotoxin by the lymphoblasts. There was close contact between blast cells and macrophages and between macrophages and lymphocytes. The close association of lymphoblasts, plasmablasts, and macrophages in the transitional areas suggests that these are sites of T- and B-cell interaction in the rheumatoid synovial immune response.

Adult↗
PubMedDate not supplied

Lymphocyte response to virus antigens in systemic lupus erythematosus.

The cell-mediated immune response of lymphocytes to rubella, measles, parainfluenza types 1, 2, and 3, varicella-zoster and herpes virus type 1 virus antigens was evaluated in 15 SLE patients and 15 matched controls by incorporating 3H-thymidine in whole blood cultures as a measure of blastic transformation. SLE patients were less responsive than normal individuals to six of eight virus antigens tested. Culture of washed SLE cells in AB plasma did not reverse the hyporesponsiveness. The results indicated that a functional impairment of the circulating lymphocytes appeared to be responsible for the in vitro hyporesponsiveness of SLE patients to virus antigens.

ABO Blood-Group System↗
PubMedDate not supplied

Transfer of spleen cells from young to aging NZB X NZW F1 hybrid mice. Effect on mortality, antinuclear antibody, and renal disease.

Multiple injections of spleen cells from young NZB x NZW F1 hybrid mice with a mean age of 9 weeks were administered to syngeneic recipients at 10-day intervals beginning at 3 months of age. The recipient mice had a dealyed appearance of antinuclear antibody, decreased cumulative incidence of antinuclear antibody positivity up to 7.3 months of age, lower cumulative mortality up to 8 months of age, and a lesser degree of glomerular sclerosis at 8 months of age. By 9 months of age no significant differences in these parameters remained. Transferred cells were most effective during the 4- to 6-week period immediately following initiation of injections. The beneficial effects appeared to reflect an early but temporary suppression of the autoimmune process by the transferred lymphoid cells.

Aging↗