Biomedical subjects

MJ Booth

Publications and source records attributed to MJ Booth.

7 recordsLinked to original sources

PubMed2000

Adaptive aberration correction in a two-photon microscope

We demonstrate aberration correction in two-photon microscopy. Specimen-induced aberrations were measured with a modal wavefront sensor, implemented using a ferro-electric liquid crystal spatial light modulator (FLCSLM). Wavefront correction was performed using the same FLCSLM. Axial scanned (xz) images of fluorescently labelled polystyrene beads using an oil immersion lens show restored sectioning ability at a depth of 28 &mgr;m in an aqueous specimen.

Journal Article↗

PubMed2000

New modal wave-front sensor: a theoretical analysis

We present a new design of a modal wave-front sensor capable of measuring directly the Zernike components of an aberrated wave front. The sensor shows good linearity for small aberration amplitudes and is particularly suitable for integration in a closed-loop adaptive system. We introduce a sensitivity matrix and show that it is sparse, and we derive conditions specifying which elements are necessarily zero. The sensor may be temporally or spatially multiplexed, the former using a reconfigurable optical element, the latter using a numerically optimized binary optical element. Different optimization schemes are discussed, and their performance is compared.

Journal Article↗

PubMed1995