Biomedical subjects

Mahasweta Das

Publications and source records attributed to Mahasweta Das.

6 recordsLinked to original sources

PubMed2007

Hypothalamic and brainstem sources of pituitary adenylate cyclase-activating polypeptide nerve fibers innervating the hypothalamic paraventricular nucleus in the rat.

The hypothalamic paraventricular nucleus (PVN) coordinates major neuroendocrine and behavioral mechanisms, particularly responses to homeostatic challenges. Parvocellular and magnocellular PVN neurons are richly innervated by pituitary adenylate cyclase-activating polypeptide (PACAP) axons. Our recent functional observations have also suggested that PACAP may be an excitatory neuropeptide at the level of the PVN. Nevertheless, the exact localization of PACAP-producing neurons that project to the PVN is not understood. The present study examined the specific contribution of various brain areas sending PACAP innervation to the rat PVN by using iontophoretic microinjections of the retrograde neuroanatomical tracer cholera toxin B subunit (CTb). Retrograde transport was evaluated from hypothalamic and brainstem sections by using multiple labeling immunofluorescence for CTb and PACAP. PACAP-containing cell groups were found to be retrogradely labeled from the PVN in the median preoptic nucleus; preoptic and lateral hypothalamic areas; arcuate, dorsomedial, ventromedial, and supramammillary nuclei; ventrolateral midbrain periaqueductal gray; rostral and midlevel ventrolateral medulla, including the C1 catecholamine cell group; nucleus of the solitary tract; and dorsal motor nucleus of vagus. Minor PACAP projections with scattered double-labeled neurons originated from the parabrachial nucleus, pericoeruleus area, and caudal regions of the nucleus of the solitary tract and ventrolateral medulla. These observations indicate a multisite origin of PACAP innervation to the PVN and provide a strong chemical neuroanatomical foundation for interaction between PACAP and its potential target neurons in the PVN, such as parvocellular CRH neurons, controlling physiologic responses to stressful challenges and other neuroendocrine or preautonomic PVN neurons.

Animals↗

PubMed2005

Dynamic changes in baroreceptor-sympathetic coupling during the respiratory cycle.

In urethane-anesthetized, paralyzed, and artificially ventilated cats, we observed an unusual form of "phase walk" of the cardiac-related burst of inferior cardiac postganglionic sympathetic nerve discharge (SND) relative to the systolic phase of the arterial pulse (AP) and thus pulse-synchronous baroreceptor nerve activity. Unlike classic phase walk ascribable to weakened coupling (desynchronization) of two oscillators, AP-SND phase walk was characterized by epochs of progressive, bidirectional changes in the angle of strong coupling (AP-SND coherence values, >0.9) of these signals that recurred on the time scale of the respiratory cycle and whose range was approximately one third of the period of the heart beat. AP-SND phase walk was linked to two respiratory variables (central respiratory drive and vagal lung inflation afferent activity) as demonstrated by the following observations. First, in five normocapnic cats (end-tidal CO(2), 4.3 +/- 0.2%) with intact vagus nerves and three vagotomized cats, AP-SND phase walk was characterized by a progressive heart-beat-to-heart-beat decrease in the lag of SND relative to the AP during the inspiratory phase of phrenic nerve activity and an increase in the lag during the expiratory phase. Second, in three cats with intact vagus nerves that were hyperventilated (end tidal CO(2), 1.6 +/- 0.4%) to phrenic nerve quiescence, the lag of the cardiac-related burst of SND relative to the AP increased during lung inflation and decreased during lung deflation. Additional experimentation revealed that AP-SND phase walk is attributable to respiratory-induced changes in the frequency of the centrally generated sympathetic nerve rhythm rather than heart rate. Moreover, the data demonstrate that the frequency and amplitude of the sympathetic oscillation are independently controlled by the above mentioned respiratory parameters.

Action Potentials↗

PubMed2005

Behavioral effects of local microinfusion of pituitary adenylate cyclase activating polypeptide (PACAP) into the paraventricular nucleus of the hypothalamus (PVN).

Pituitary adenylate cyclase activating polypeptide (PACAP) has been implicated in the regulation of several autonomic and neuroendocrine functions. In the hypothalamic paraventricular nucleus (PVN), for example, PACAP-immunoreactive fibers densely innervate corticotropin-releasing hormone (CRH)-containing neurons in the medial parvocellular region, suggesting that PACAP acts to mediate stress responses. Therefore, we examined the behavioral effects of an intra-PVN PACAP injection (25 pmol) in combination with a mild stressor. PACAP or artificial cerebrospinal fluid (aCSF) was microinjected into the PVN (0.25 l) and then animals were restrained or placed in their home cage for 5 min. Exploratory activity (total distance traveled) and scored behaviors (face washing, body grooming, wet dog shakes, and rearing) were observed in a familiar open field for 10 min. In animals receiving aCSF, there were no behavioral differences between restrained and unrestrained groups. For the entire 10-min observation period, animals receiving PACAP, whether restrained or not, displayed elevated face washing and body grooming with decreased locomotor activity and rearing. Among PACAP-injected animals, restrained animals displayed increased body grooming compared to unrestrained animals during the first 2 min in the open field suggesting a summation of the effects of peptide injection and stressor. The observed elevation in grooming is consistent with previous studies reporting similar increases following electrical-, NMDA-, CRH-, or stressor-induced activation of the PVN. Thus, at the level of the PVN, PACAP may act as an excitatory neuropeptide and augment behavioral responses to stressors.

Animals↗

PubMed2004

An unusual form of phase walk in a system of coupled oscillators.

The authors describe an unusual form of phase walk (i.e., a progressive change in phase angle between coupled oscillators) using the 10-Hz rhythmic discharges of the inferior cardiac and vertebral postganglionic sympathetic nerves (CN and VN, respectively) in hypercapnic, baroreceptor-denervated, and vagotomized cats anesthetized with urethane. Unlike phase walk ascribable to weakened coupling (desynchronization of oscillators), the phase walk of VN 10- Hz activity relative to CN10-Hz activity 1) recurred on the time scale of the respiratory cycle, 2) was bidirectional with CN-VN phase angle increasing during expiration and decreasing during inspiration, and 3) occurred over a range equivalent to one-half the period of the 10-Hz rhythm rather than a full cycle. Moreover, this form of phase walk occurred during strong coupling of the 10-Hz oscillators, as reflected by CN-VN coherence values approaching 1.0. The authors propose that the bidirectional phase walk reflects a state of strong coupling of the 10-Hz oscillators controlling the CN and VN, the angle of which is reset from cycle to cycle by the continuously changing level of activity in their respiratory inputs. In addition, the data demonstrate that frequency and amplitude modulation of sympathetic nerve discharge can be independently regulated by respiratory inputs.

Animals↗

PubMed2003

Fractal activity generated independently by medullary sympathetic premotor and preganglionic sympathetic neurons.

In anesthetized cats with cervical spinal cord transection, Fano factor analysis was used to test for time-scale invariant (fractal) fluctuations in spike counts of single preganglionic cervical sympathetic neurons (PSNs) and putative sympathetic premotor neurons located in the rostral ventrolateral medulla (RVLM) and caudal medullary raphe. The medullary neurons exhibited cardiac-related activity, and their axons projected to the spinal cord, as demonstrated by antidromic activation. The variance-to-mean spike count ratio (Fano factor) was plotted as a function of the window size used to count spikes. The Fano factor curves for seven PSNs, eight RVLM neurons, and eight raphe neurons contained a power law relationship extending over more than one time scale. In these cases, random shuffling of the interspike intervals in the original time series eliminated the power law relationship. Thus the power law relationships can be attributed to long-range correlations among interspike intervals rather than simply to the distribution of the intervals that is not changed by shuffling the data. It is concluded that PSNs and sympathetic premotor neurons in the medulla can independently generate fractal firing patterns.

Action Potentials↗

PubMed2003

Fractal properties of sympathetic nerve discharge.

Fano factor analysis and dispersional analysis were used to characterize time series of single and multifiber spikes recorded from the preganglionic cervical sympathetic nerve and cardiac-related slow-wave activity of the whole postganglionic sympathetic vertebral nerve (VN) in anesthetized cats. Fluctuations in spike counts and interspike intervals for single preganglionic fibers proved to be fractal (i.e., time-scale invariant), as reflected by a power law relationship between indices of the variance of these properties and the window size used to make the measurements. Importantly, random shuffling of the data eliminated the power law relationships. Fluctuations in spike counts in preganglionic multifiber activity also were fractal, as were fluctuations in the height and of the area of cardiac-related slow waves recorded from the whole postganglionic VN. These fractal fluctuations were persistent (i.e., positively correlated), as reflected by a Hurst exponent significantly >0.5. Although fluctuations in the interval between cardiac-related VN slow waves were random, those in the interval between heart beats were fractal and persistent. These results demonstrate for the first time that apparently random fluctuations in sympathetic nerve discharge are, in fact, dictated by a complex deterministic process that imparts "long-term" memory to the system. Whether such time-scale invariant behavior plays a role in generating the fractal component of heart rate variability remains to be determined.

Action Potentials↗