A new principle in combinatorial asymmetric transition-metal catalysis: mixtures of chiral monodentate P ligands.
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Journal Article↗
Biomedical subjects
Manfred T Reetz
Publications and source records attributed to Manfred T Reetz.
Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.
A new and practical method for gene recombination with formation of libraries of mutant genes is presented. The method is based on the assembly of appropriately prepared oligonucleotides whose design is guided by sequence information. High recombination frequency with formation of full-length products is achieved by controlled overlapping of the designed oligomers. This process (ADO) minimizes self-hybridization of parental genes, which constitutes a significant advantage over conventional family shuffling as used in the directed evolution of functional enzymes. ADO was applied to the recombination of two lipase family genes from Bacillus subtilis (LipA and LipB). In a library of 3000 lipase variants created by this method, several were found that display increased enantioselectivity in a model reaction involving the hydrolysis of a meso-diacetate.
Amino Acid Sequence↗
An overview of high-throughput screening systems for enantioselective enzymatic transformations.
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Catalysis↗
Select protocols of high-throughput ee-screening systems for assaying enantioselective enzymes.
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Automation↗
A screening system for enantioselective enzymes based on differential cell growth.
The esterase-catalyzed enantioselective hydrolysis of the fluoroacetate of pantolactone leads to fluoroacetic acid, a toxic compound which inhibits the growth of esterase-producing yeast; this forms the basis of an ee-assay.
4-Butyrolactone↗
Biocatalysis in ionic liquids: batchwise and continuous flow processes using supercritical carbon dioxide as the mobile phase.
A new and environmentally benign protocol for enzymatic reactions in ionic liquids is described using supercritical CO2 as the mobile phase; the products are obtained in solvent-free form and the enzyme/ionic liquid mixture can be recycled in batchwise or continuous flow operations.
Candida↗
Lipases as practical biocatalysts.
Lipases are the most used enzymes in synthetic organic chemistry, catalyzing the hydrolysis of carboxylic acid esters in aqueous medium or the reverse reaction in organic solvents. Recent methodological advancements regarding practical factors affecting lipase activity and enantioselectivity are reviewed. Select practical examples concerning the use of lipases in the production of chiral intermediates are also highlighted.
Catalysis↗