Biomedical subjects

Manuel Rodríguez-Martínez

Publications and source records attributed to Manuel Rodríguez-Martínez.

3 recordsLinked to original sources

PubMed2006

Gingivitis and periodontitis as antagonistic modulators of gingival perfusion.

BACKGROUND: We explore the possible association between the extent of gingivitis or periodontitis and an index of gingival microvascular perfusion response to compression of alveolar mucosa, called the gingival perfusion index (GIPI). METHODS: A cross-sectional analytical study was done in a sample of 60 adults, including healthy and non-insulin-dependent diabetic subjects of either gender, with teeth in the anteromandibular sextant with or without gingivitis and with or without periodontitis at the lower-left lateral incisor (LLLI). A sample was selected by convenience non-probability sampling. Gingival perfusion was evaluated at labial LLLI attached gingiva. Two perfusion recordings were done 5 minutes apart, each one consisting of a 40-second control phase, a 22-second compression phase, and a 40-second postcompression phase. LLLI alveolar mucosa was compressed with a wood-mounted cotton swab until reaching about one-fifth of the control perfusion value. GIPI was used as response dependent variable. The gingival index and probing depth were used as measures of the extent of gingivitis and periodontitis, respectively. RESULTS: By analysis of covariance and multiple regression analysis, it was found that only the probing depth (negatively) and gingival index (positively) predicted GIPI (R(2) adjusted = 0.5194, P <0.0001). CONCLUSIONS: The data strongly suggest that, at least in the studied sample, gingivitis and periodontitis operate as antagonistic modulators of gingival perfusion. The major practical implication of our findings is that an increase or decrease in this index (GIPI) at a given attached gingiva site could indicate, respectively, the clinical predominance of gingivitis or periodontitis in such a site.

Adult↗

PubMed2005

A novel procedure for evaluating gingival perfusion status using laser-Doppler flowmetry.

OBJECTIVES: To systematize a procedure that allows one to characterize the perfusion response pattern of attached gingiva to the topical and transitory compression of alveolar mucose, using laser-Doppler flowmetry. MATERIAL AND METHODS: A cross-sectional study was carried out, in 20 healthy adult subjects of either sex, with teeth in antero-mandibular sextant but without periodontitis at the lower left lateral incisor (LLLI). Sample was selected by convenience non-probability sampling. Gingival perfusion was evaluated at labial LLLI attached gingiva using a specially designed gingival tray. Two perfusion recordings were carried out 5 min. apart, each one consisting of a 40 s control phase, a 22 s compression phase and a 40 s post-compression phase. During compression phase, LLLI alveolar mucose was compressed with a wood-mounted cotton swab until the perfusion decreased to about 1/5 of its control perfusion value. RESULTS: Integrated primary basal flow (IPBF) during control phase was of 14,210+/-1075 perfusion units (PU), whereas integrated flow during compression phase was of 1651+/-202 PU (p<0.05). After compression was released, integrated total secondary real flow was 13,322+/-1513 PU (p<0.05) which represented a 91.3+/-3.8% of IPBF. Gingival compression propitiated an induced flow debt (IFD) of 6478+/-781 PU, which increased in 980+/-482 PU after compression was released, representing 18% of the IFD (Debt index). CONCLUSIONS: A hypoaemic response in reaction to topical and transitory LLLI alveolar mucose compression was observed. Debt index and the ITSRF% are reproducible indices of microvascular perfusion response whose validation under pathological circumstances remains to be evaluated.

Adult↗

PubMed2003

Fluoride-induced disruption of reproductive hormones in men.

Fluoride-induced reproductive effects have been reported in experimental models and in humans. However, these effects were found in heavily exposed scenarios. Therefore, in this work our objective was to study reproductive parameters in a population exposed to fluoride at doses of 3-27 mg/day (high-fluoride-exposed group-HFEG). Urinary fluoride levels, semen parameters, and reproductive hormones in serum (LH, FSH, estradiol, prolactin, inhibin-B, free and total testosterone) were measured. Results were compared with a group of individuals exposed to fluoride at lower doses: 2-13 mg/day (low-fluoride-exposed group-LFEG). A significant increase in FSH (P<0.05) and a reduction of inhibin-B, free testosterone, and prolactin in serum (P<0.05) were noticed in the HFEG. When HFEG was compared to LFEG, a decreased sensitivity was found in the FSH response to inhibin-B (P<0.05). A significant negative partial correlation was observed between urinary fluoride and serum levels of inhibin-B (r=-0.333, P=0.028) in LFEG. Furthermore, a significant partial correlation was observed between a chronic exposure index for fluoride and the serum concentrations of inhibin-B (r=-0.163, P=0.037) in HFEG. No abnormalities were found in the semen parameters studied in the present work, neither in the HFEG, nor in the LFEG. The results obtained indicate that a fluoride exposure of 3-27 mg/day induces a subclinical reproductive effect that can be explained by a fluoride-induced toxic effect in both Sertoli cells and gonadotrophs.

Adult↗