Biomedical subjects

Manuela Stancu

Publications and source records attributed to Manuela Stancu.

3 recordsLinked to original sources

PubMedDate not supplied

Aspects of the humoral and cellular immune response in hepatitis associated with cholelithiasis.

AIM: To investigate the immunological reactions of the liver in various gall bladder disease. METHODS: IgG, IgA and IgM were measured in the blood and bile of patients with cholecystectomy. Immunofluorescence and electron microscopy techniques were used. RESULTS: The tests showed high blood values of immunoglobulins and their presence in the bile. The immunofluorescence was positive for IgG in the hepatic sinusoids. The electron microscopy showed aspects of "cellular cooperation". CONCLUSIONS: On the basis of our results we can affirm that in cholelithiasis there exists an immune response to the persistent antigens, with the release of circulating immune complexes.

Bile↗

PubMedDate not supplied

[New data about hepatic sinusoidal endothelial cell].

Liver sinusoids are irregularly dilated capillaries. The sinusoidal lining consists of a sample squamous epithelium. Endothelial cells constitute the major cellular element of the sinusoidal lining and form a discontinuous endothelium. These cells are separated by gaps 0.1-0.5 mm wide. The endothelial cells also show numerous intracellular pores or fenestrations. They perform important filtration function due to the presence of small fenestrations that allow free diffusion of many substances and show huge endocytic capacity. Liver sinusoidal endothelial cells may function as antigen-presenting cells (APC) and are also active in the secretion of cytokines, eicosanoids, endothelin-1, nitric oxide and some extracellular matrix components. They can store and metabolize substances as serum immunoglobulins, too.

Capillaries↗

PubMedDate not supplied

[The detection of dysplastic lesions of the gallbladder mucosa in polypoid cholelithiasis].

AIM: To investigate the hyperplastic and/or dysplastic lesions in the gallbladder mucosa. METHODS: We used the immunohistochemical Ardeleanu-Hsu-Bussolati and Gugliotta methods, with avidin and peroxide biotin, adapted in the laboratory of University Hospital "St. Spiridon" Iaşi. There were studied ten cases of polypoid cholelithiasis, with the following markers: Ki67, PCNA, and p53. RESULTS: The immunohistochemical methods using Ki67, PCNA and p53 protein allowed the evaluation of the abnormal proliferation abilities characteristic for some hyperplastic or dysplastic lesions, found in our cases of cholelithiasis. CONCLUSIONS: The utilization of these markers permitted us to establish a good correlation between the high activity of Ki67 and the morphological aggression indicators in hyperplastic and dysplastic gallbladder epithelium. There is also a correlation between the percentage of the cells marked with anti-PCNA antibody and the existence of p53 protein in these lesions.

Biomarkers, Tumor↗