PubMed HealthSearch

Biomedical subjects

Marco Bürger

Publications and source records attributed to Marco Bürger.

2 recordsLinked to original sources

Painting new pathways: Castilleja enters the genomic era.

Castilleja (Orobanchaceae), commonly known as Indian paintbrush, is a genus of approximately 200 hemiparasitic species found primarily across the Americas. Long studied for its taxonomic complexity, vibrant floral displays, and ecological interactions with host plants, Castilleja has recently emerged as a versatile research system spanning parasitic biology, specialized metabolism, conservation genetics, and pharmacology. The availability of whole-genome sequencing is transforming the field, revealing expanded gene families involved in host recognition, enabling genomic species delimitation of cryptic taxa, and providing frameworks for mapping biosynthetic pathways of bioactive compounds, including iridoid glycosides and phenylethanoid glycosides. This review synthesizes advances across these disciplines and highlights how genomics serves as an integrative force connecting taxonomy, ecology, phytochemistry, and parasitic biology in this genus.

Castilleja

Genomes of Conopholis americana and Epifagus virginiana: two holoparasitic plants (Orobanchaceae).

Conopholis americana (American cancer-root) and Epifagus virginiana (beechdrops) are sister genera of holoparasitic plants (Orobanchaceae) native to eastern North America, parasitizing oaks and American beech, respectively. Both have served as models for plastid genome reduction, yet no nuclear genomes exist for either genus or any New World holoparasitic Orobanchaceae. Here we present the first nuclear genome assemblies for both species using PacBio HiFi sequencing. The C. americana assembly totals 1.82 Gb and E. virginiana totals 440 Mb, representing an approximately 4-fold difference in genome size between these sister genera. We observed a BUSCO completeness of 79% to 80% in both species, which is typical of holoparasites. While gene prediction identified 33,889 genes in C. americana and 21,031 in E. virginiana, repeat annotation revealed that LTR retrotransposons account for 78% of the genome size difference. These assemblies reveal contrasting mechanisms of genome evolution in sister holoparasitic genera and provide foundational resources for comparative genomics of parasitic plants.

Genome, Plant