Biomedical subjects

Margit Cichna-Markl

Publications and source records attributed to Margit Cichna-Markl.

3 recordsLinked to original sources

PubMed2007

Selective sample cleanup by reusable sol-gel immunoaffinity columns for determination of deoxynivalenol in food and feed samples.

The paper describes the development of a simple and highly selective method for the determination of deoxynivalenol (DON) in food and feed samples. It combines sample cleanup with sol-gel immunoaffinity columns containing monoclonal anti-DON antibodies and quantification of DON by high-performance liquid chromatography with ultraviolet detection. The sol-gel immunoaffinity columns are as selective as commercial DON immunoaffinity columns but superior with regard to production costs, storage stability, and reusability. In applying the method for the analysis of maize, wheat, and spaghetti samples, it offers detection limits (LOD, S/N = 3) of 240, 200, and 207 ng/g, and recoveries of 83, 99, and 97%, respectively.

Antibodies, Monoclonal↗

PubMed2006

Sample clean-up with sol-gel enzyme and immunoaffinity columns for the determination of bisphenol A in human urine.

The paper describes the development of a simple and highly selective analytical method for the determination of free and total bisphenol A in urine samples. Free bisphenol A levels can be determined after sample clean-up using sol-gel immunoaffinity columns containing anti-bisphenol A antibodies. In determining total bisphenol A levels, the sample pre-treatment procedure consists of sample preparation using an on-line combination of two sol-gel columns, an enzyme column containing glucuronidase and arylsulfatase, and an immunoaffinity column. Bisphenol A can then be quantified by high-performance liquid chromatography and fluorescence detection. The mean recovery was found to be 78% with a standard deviation of 3.4%, the LOD (S/N=3) was 0.2 ng/ml. The method was applied to determine free and total urinary BPA levels of healthy adults and dialysis patients.

Adult↗

PubMed2006

Selective sample preparation with bioaffinity columns prepared by the sol-gel method.

Bioaffinity materials prepared by entrapping highly selective bioligands in the pores of a sol-gel glass offer unique advantages for the clean-up of complex sample matrices. The inclusion of sol-gel bioaffinity columns frequently allows the efficient elimination of interfering matrix components and enrichment of analytes in a simple step. After introducing the basic principles the paper reviews the methods for the production of protein-doped sol-gel materials and discusses the characteristics of sol-gel affinity columns by comparing their stability, selectivity, binding capacity and reusability. The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis.

Chromatography, Affinity↗