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Biomedical subjects

Mariusz Kaczmarek

Publications and source records attributed to Mariusz Kaczmarek.

4 recordsLinked to original sources

Burn depths evaluation based on active dynamic IR thermal imaging--a preliminary study.

Proper diagnostic assessment of burn wound depth is of the highest importance in selecting the mode of burn wound treatment. Several diagnostic methods--clinical and histopathological evaluation, as well as methods employing IR imaging--static thermography and active dynamic thermography (ADT)--are compared on the basis of in vivo experiments conducted on three domestic pigs (23 burn wounds). ADT is presented here as a new, reliable and quantitative method of assessing burn wound depth on the basis of discrimination of the thermal properties of burnt tissue. In the case of ADT registration of thermal images was performed following thermal pulse excitation. A series of captured infrared images was used as the basis for calculating the thermal time constant tau for each pixel. The parameter values were compared with histopathological and clinical assessments of burn depth. The mean value of tau was found for burns, which heal within 3 weeks (tau=12.08+/-1.94s) and for burns, which did not heal during this period (tau=9.07+/-0.68s), p<0.05. The accuracy, sensitivity and specificity of all the methods tested were compared, the best results coming from ADT. The ADT method is fast, non-invasive and relatively inexpensive, although it still requires further animal experimentation as well as clinical study to confirm the results.

Animals↗

[The role of NF-kappaB transcription factor in chronic viral hepatitis C and B].

Essential data pertaining to the structure and function of selected transcription factors such as NFAT, AP-1 and NF-kappaB in particular, are presented in the relation to viral hepatitis C and B. In chronic hepatitis C the activation of NF-kappaB is markedly modulated by viral proteins such as core protein and nonstructural ones, particularly NS5A and NS3. In hepatitis B the major factor influencing NF-kappaB function appears to be HBx protein. Effects of viral proteins on NF-kappaB function in relation to the course of hepatitis are complex. They participate in the perpetuation of inflammatory state in the liver, inhibit apoptosis of hepatocytes, as well as the differentiation of antigen-presenting cells (dendritic ones). The latter effect has deleterious impact on the formation of specific immune response to viral peptides. It seems that both viruses, C and B acquired the ability to modify NF-KB function in advantage for the pathogens in question.

Animals↗

Static thermography revisited--an adjunct method for determining the depth of the burn injury.

The aim of the study was to investigate the relationship between the static thermography figure of merit DeltaT (the difference in mean values of skin area temperature for the burn wound area and the unaffected reference skin area) and a means of burn classification which would be most suitable for the choice of treatment. The work was an in vivo animal experiment. Statistical analysis showed a high correlation between the DeltaT parameter and histopathological assessment. With regard to the choice of treatment, the most useful correlation was found to be that between DeltaT and the classification of burn wounds into those healed in 3 weeks and those unhealed. The results of this study have revealed a quantitative criterion DeltaT for burn classification. The study suggests that particular burn centres using static thermography use a DeltaT parameter based on their own values for burn classification so as to group burn wounds into those that healed in 3 weeks and those that did not heal. This criterion should be independent of and replace other classification systems. A criterion for the proper choice of burn treatment would then be made more readily available.

Animals↗

[Alterations within white blood cell subsets in children with chronic viral hepatitis C before treatment with pegylated interferon and ribavirin].

OBJECTIVE: Detection of differences in white blood cell subsets I children with chronic viral hepatitis type C before antiviral treatment. METHODS: A cohort of children (n = 52) with proven HCV infection were subjected to flow cytometric analysis of their white blood cells and compared to non-infected control group. MAIN OBSERVATIONS: It has been found that the hepatitis group has higher number of cells with cytotoxic potential, decreased CD4/CD8 ratio than the other one. RESULTS: Significant rise of CD8+, CD28- cells, NK cells and NKT lymphocytes was demonstrated in hepatitis group. Several correlations were noticed between various cell subsets studied in virus C infected children. CONCLUSIONS: This data show that HCV infection affects child immunity at systemic level. Cellular alterations are detectable by means of flow cytometry. Evaluation of its parameters might have predictive value in antiviral treatment.

Adolescent↗