Biomedical subjects

Mariusz Sacharczuk

Publications and source records attributed to Mariusz Sacharczuk.

4 recordsLinked to original sources

PubMed2004

A study for minisatellitic markers of Conidiobolus coronatus' pathogenicity to Galleria mellonella larvae.

A selected panel of 13 colonies of entomopathogenic fungus Conidiobolus coronatus representing 6 variants of pathogenicity to Galleria mellonella larvae (ranged from 100 to 10% of efficiency), derived from single spores, were tested for the presence of hypervariable loci in their genomes by hybridization with Jeffreys' human minisatellite probe 33.6. The probe produced informative fingerprints and revealed slight differences among colonies analyzed. Up to 20 variable bands per colony were recognized in the size range of 2-20 kb. The band sharing within groups with the same pathogenicity ranged from 0.966 to 0.800. The genetic distance between different variants ranged from 0.026 to 0.282. A few characteristic bands for high and low pathogenicity to the larvae were found.

Animals↗

PubMed2003

Cytogenetic comparison of the sensitivity to mutagens in mice selected for high (HA) and low (LA) swim stress-induced analgesia.

Sensitivity to mutagens was studied in mouse lines selectively bred for high analgesia (HA) and for low analgesia (LA) induced by 3-min swimming in 20 degrees C water. Apart from pain-related traits HA mice also manifest, as compared to the LA line, higher emotionality in various behavioural tests, and cope worse with the hypothermic challenge of swimming in cold water. In the present study HA mice appeared more susceptible to the mutagenic effect of whole-body gamma-radiation and mitomycin-C injection. Both treatments caused higher frequencies of chromosomal aberrations and micronucleus in bone marrow cells in the HA than in the LA line. The results are discussed in terms of a genetic correlation between animals' susceptibility to environmental stressors and the mechanism of mutagenesis. As shown by our recent molecular study, the selection for magnitude of swim analgesia has differentiated the parental outbred population into two distinct genotypes characterised by specific minisatellite and microsatellite sequences for each line, which may be genetic markers of particular traits. We conceive that the breeding strategy, along with the differentiation of stress-related phenomena, has altered the frequencies of genes controlling DNA repair in each line.

Analgesia↗

PubMed2003

Chromosomal NOR activity in mice selected for high and low swim stress-induced analgesia.

Metaphase nucleolar organizer region (NOR) activity was studied in mouse lines selectively bred for high analgesia (HA) and for low analgesia (LA) induced by 3-min swimming in 20 degrees C water. Apart from pain-related traits, HA mice also manifest, as compared to the LA line, higher emotionality in certain behavioral tests and are less capable of coping with the hypothermic challenge of swimming in cold water, in addition to being more susceptible to the mutagenic effect of whole-body gamma-radiation and mitomycin C injection. We here compared NOR activity in HA and LA mice. A statistically significant difference (p < or =.01) was detected in mean silver-stained nucleolar number/cell between the HA and LA lines, being lower for HA mice. We propose that the breeding strategy, along with the differentiation of stress-related phenomena, has altered the activity of genes coding rRNA and that this activity is important in controlling DNA repair in each line. It is concluded that quantitative studies of nucleolar changes may be useful in evaluating the biological reactivity to stress stimuli.

Analgesia↗

PubMedDate not supplied

[Lactoferrin in the central nervous system].

Lactoferrin (LF) is a protein secreted by the tissues of ectodermal origin. Its structure is similar to transferrin. LF appeared to be multifunctional, but its main functions are connected with the natural defense system of mammals. The biological role and origin of LF within brain in normal and disease processes are as yet uncharted. LF expression is greatly upregulated during neurodegenerative disorders and in elderly brains. LF may exert an antiinflammatory function via its inhibitory effect on hydroxyl radical formation. By antioxydative properties, LF prevents DNA damage and consequently tumor formation in the CNS. Moreover, LF specifically transactivates the p53 tumor suppressor gene. LF suppresses distress perception via opioid mediated mechanism and prevents a decrease of the immune system activity caused by psychosocial stress. Furthermore, LF possibly modulates behavior in man and in animals.

Alzheimer Disease↗