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Mariusz Skoczyński

Publications and source records attributed to Mariusz Skoczyński.

4 recordsLinked to original sources
PubMed2006

[Prevalence of Leptotrichia amnionii sp. nov. in pregnant women].

INTRODUCTION: Leptotrichia bacteria belong to the group of Gram-negative anaerobes most frequently colonizing the oral cavity and reproductive organs. Leptotrichia sanquigenes constitute one of the bacterial factors in postpartum bacteremia of mothers and newborns, whilst Leptotrichia amnionii sp. nov., described for the first time in 2002, has turned out to be the etiopathogenetic factor in missed abortion in the second trimester of pregnancy. AIM: The purpose of this study was evaluation of the frequency of occurrence of Leptotrichia amnionii sp. nov. in patients in the 1st, 2nd and 3rd trimester of pregnancy. MATERIALS AND METHODS: The study involved 69 patients. Amplification reactions detecting the presence of Leptotrichia amnionii so. RNA in the smears were performed using specific starters complementary to 16SrRNA Leptotrichia amnionii--primer 1 Lam upper and primer 2 Lsp lower--complementary to Leptotrichia 16SrRNA. After amplification, the specimens were placed on 1% agarose gel in the presence of MassRuler marker (Fermentas). Next, PCR products were ligated to pGEM-T Easy Victor and then competent E. coli DH5 alpha cells were transformed with pGEM vector with an insert. Plasmid DNA isolation was performed using Plasmid Mini kit from A&A Biotechnology. Sequencing of inserts from the purified plasmids was performer in Molecular Biology Techniques Laboratory in the Department of Biology at Adam Mickiewicz University in Poznań. RESULTS: In the group of 69 pregnant patients Leptotrichia amnionii sp. nov. was identified in 8 women, which is 11.6%. CONCLUSION: Recently identified Leptorichia amnionii sp. nov. is a bacterie, which relatively frequently occurs in pregnant women.

Adult↗
PubMed2004

Forceps delivery after two previous Caesarean sections.

Caesarean section is one of the most frequently performed surgical procedures in current obstetric practice. There are several reasons for this upward tendency, including well developed hemotherapy, anesthesia, antibiotic treatment and skilled doctors. Despite such risks as uterine rupture, necessity of blood transfusion or the possibility of the necessity of ending the delivery by means of surgery, it is possible, in justified cases, to attempt spontaneous labor. A decision jointly made by the patient and her doctor, based on careful consideration of benefits and risks, as well as positive attitude on the woman's part may lead to positive outcome of labor. Taking the above factors into account we have decided to present a study of a difficult, operational labor after two Caesarean sections.

Adult↗
PubMed2002

[Influence of labetalol on the resistance of human fetoplacental vessels in perfusion in vitro].

OBJECTIVES: The main unfavourable effects observed in gestosis include: increased vascular peripheral resistance and increased blood pressure with a defective uteroplacental flow. The above mentioned changes may result from disorders in prostacyclin PGI2/thromboxane A2 balance. DESIGN: The aim of the studies presented was to compare the effects of, labetalol on the fetal vascular resistance induced by U 46,619--stable analogue of thromboxane A2 an experimental bilateral perfusion of the human placental lobule. MATERIALS AND METHODS: The researchers used the experimental model described by Schneider. 12 perfusions of the human placental lobules were performed. The control and labetalol groups consisted in 6 placental lobule perfusions lasting 120 min each. Having obtained constant increase in perfusion pressure from the 60th min of the experiment, labetalol was administered along with thromboxane A2 analogue into the fetal circulation. The perfusion pressure was steadily recorded on the kymograph tape. RESULTS: The stable increase of perfusion pressure was observed from the 60th minute with the average of 185% of the initial pressure. This value represented the reference standard in the second stage of the studies in which the effects of labetalol on the experimentally increased perfusion pressure was evaluated. CONCLUSIONS: Labetalol did not significantly affect the hemodynamics of fetoplacental vessels.

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗