Biomedical subjects
Publications and source records attributed to Marvin J Robinson.
Increased immunoactivity of (-nitrosolysine was detected in Western blot in peroxynitrite-reacted histones H2A, H3 and H4. The N-nitrosation may be reversible as indicated by the decreased immunoactivity via either subsequent incubation with reduced glutathione or 48-hour dialysis and 48-hour storage at 4 degrees C. Nonetheless, the biological significance of N-nitrosation of protein lysine is yet to be proven.
At neutral pH, S-nitrosoglutathione was formed by the reaction of reduced glutathione and sodium nitrite. The degradation of S-nitrosoglutathione, presumably by transnitrosation/denitrosation, was catalyzed by L-cysteine, or CoA-SH. Additionally, from the crude extract of rat brain, one protein with a large molecular mass was nitrosolated with nitrite, and was split into duplet peptides noted in Western blot. Furthermore, the incubation of nitrite with IgG may generate the intermediates of active nitrogen/oxygen species and lead to significant production of gas bubbles.