Biomedical subjects

Marzenna Bartoszewicz

Publications and source records attributed to Marzenna Bartoszewicz.

4 recordsLinked to original sources

PubMed2005

[Fenotypical characteristic of coagulase negative staphylococci isolated from patients hospitalized on burn ward and surgical ward].

The aim of the study was to analysis of the adhesion factors of coagulase-negative staphylococci (CNS) to intravenous catheter: ability to adhesion, slime production, hydrophobicity of surface of cells and susceptibility to antibiotics. The researches heve been done on CNS strains isolated from cardiosurgical patients. Slime production ability was evaluated using plate method according to Christensen and Congo Red Agar method. Adherence of bacterial strains to intravenous catheter (polytetrafluoroethylene) in vitro was determined using the method of Richards. Hydrophobicity of surface of cells was determined on the basis of agregattion in (NH4)2SO4 Susceptibility to antibiotics was determined using the disc-diffusion method. Out of the analyzed strains 19% were labeled as slime producing in the plate method and 14% in the Congo red Agar method. 43% of analyzed strains were found to have TTC reduction of 3+, 19% of 2+ and 28% of 1+. Among these 10% of the assesed strains did not reduce TTC.

Bacterial Adhesion↗

PubMed2004

[Risk of coagulase-negative staphylococci infections in catheterized patients due to adhesion and slime production].

UNLABELLED: Coagulase-negative staphylococci are important causes of infections associated with biomaterials, e.g. catheters. Aim of the study was to determine pathogenic traits of S. epidermidis: slime producing and adhesion to biomaterials. MATERIALS AND METHODS: The researches have been done on S. epidermidis strains isolated from newborns hospitalized on BCU. Defining an ability of slime production was done by means plate method--according to Christensen. Adhesive abilities of the strains to biomaterials were analyzed by means of Richard's and co. method which consist in estimating the level of a substrates's reduction--TTC (2,3,5-triphenyltetrazolium chloride) to an insoluble formazan. RESULTS: Among 41 S. epidermidis strains 68% labeled as slime producing and 32% as non-producing. Most of slime producing strains showed reduction value of TTC at +4 and +3. All non-producing S. epidermidis strains showed reduction value of TTC at 2+. CONCLUSION: The strains producing slime displayed greater adhesive abilities in comparison to the non-producing ones.

Bacterial Adhesion↗

PubMed2004

[Adhesion dependence of coagulase-negative staphylococci to biomaterials and PIA polysaccharide synthesis on icaADBC operon presence].

Both Staphylococcus epidermidis and Staphylococcus haemolyticus are important causes of infections associated with catheters and other medical devices. This infections result in significant morbidity, mortality and economic cost. It has recently been shown that not only S. epidermidis but also S. haemolyticus can produce slime and carries the ica operon responsible for and slime production. In the operon, coexpression of icaA and icaD is required for full slime synthesis. This study is focused on detecting icaA and icaD genes in S. haemolyticus and comparison of these two species. It turned out that strain representatives within the same species behave very differently and a single tested strain from each species is unlikely to be representative of the species as a whole. Contrary to S. epidermidis, S. haemolyticus strain appeared to carry no icaA-like and icaD-like genes, but was able to form biofilm in vitro.

Biofilms↗

PubMed2003

[Selected features determine pathogenicity of Staphylococcus haemolyticus].

The study have been done on S. haemolyticus strains isolated from patients hospitalized an Surgical Unit. Aim of the study was to determine pathogenic traits of S. haemolyticus: slime producing, adhesion to biomaterials, antibiotics susceptibility and the profiles of surface proteins. Among 44 S. haemolyticus strains, in the test-tube method, there have been 38% labeled as slime producing and 62% as non-producing. In the plate method at 48% slime production was noticed, while 52% strains did not produce slime. It is quite significant that all CNS strains which have an ability to produce mucus, that was proved by means of two methods (test-tube and plate), show a high level of TTC's reduction to formazan. The analysis of resistance to antibiotics in relation to slime production demonstrated more frequent antibiotic resistance of the slime-producing strains.

Animals↗