Biomedical subjects

Mei-li Liu

Publications and source records attributed to Mei-li Liu.

2 recordsLinked to original sources

PubMed2004

[Effect of jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes].

OBJECTIVE: To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I(Ca-L)) in ventricular myocytes of guinea pigs. METHODS: Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417. RESULTS: 311 (10, 25, 50, 100 mmol/L) increased the (I(Ca-L)) by 8.27%, 27.29%, 41.01%, and 48.74% (P < 0.05), respectively. 417 (10, 25, 50, 100 mmol/L) increased the (I(Ca-L)) by 10.05%, 30.12%, 43.05%, and 51.90% (P < 0.05), respectively. Both 311 and 417 changed the (I(Ca-L)) significantly in a concentration-dependent manner. They did not change the shape of I-V cruves. CONCLUSION: 311 and 417 can increase I(I(Ca-L)) n ventricular myocytes of guinea pigs in a dose-response manner.

Animals↗

PubMed2003

[Surface morphology of CD34+ cells under atomic force microscope].

OBJECTIVE: To observe the difference in surface morphology and structure between CD34(+) cells from normal human subjects and from patients with leukemia. METHODS: Bone marrow mononuclear cells from 3 normal human subjects and 3 patients with M2b leukemia were collected by Percoll gradient centrifugation, followed by purification of CD34(+) cells by means of immunomagnetic bead separation (MiniMAC) and examination with flow cytometry. The morphology of the cells were then observed with optical and atomic force microscope (AFM) in air. RESULTS: No significant difference was identified between the two cells under optical microscope. With atomic force microscope, numerous microvilli were observed on the surface of CD34(+) cells, and the normal cells and those from the leukemic patients showed significant difference in terms of the roughness of the cell surface. CONCLUSION: Normal CD34(+) cells have more rough and erosive surface structure than leukemic CD34+ cells (M2b).

Antigens, CD34↗