Biomedical subjects

Meng-Li Tsai

Publications and source records attributed to Meng-Li Tsai.

4 recordsLinked to original sources

PubMed2006

Frequency response characteristic of sympathetic mediated low-frequency blood pressure fluctuations in conscious rats.

A quantitative relationship between power densities of blood pressure (PBP) and sympathetic nerve activity (PSNA) in a low-frequency range (LF, 0.016-0.85 Hz), expressed as PSNA=PBPxax10bx(frequency) was proposed in pentobarbital-anesthetized rats. For evaluating the general applicability of this equation, the quantitative relationship of power density ratio Hf=PBP/PSNA across frequency was tested in a conscious state. Wistar rats were chronically instrumented with a femoral artery catheter and recording electrode around the renal sympathetic nerve. The blood pressure and renal sympathetic nerve activity were monitored both under pentobarbital anesthesia and in a conscious state. Linear regression analysis of the relationship between the frequency and logarithmic magnitude of the power density ratio in the LF range revealed excellent fit in both conditions (r=-0.96+/-0.01 and -0.93+/-0.01 for anesthetized and conscious rats, respectively). Comparing the regression lines, rats under pentobarbital anesthesia had significantly larger values for the y-intercept and slope compared to rats in a conscious state (y-intercepts: 0.80+/-0.09>0.53+/-0.08; slopes: -2.86+/-0.26>-1.62+/-0.21). Our results demonstrate that it is also feasible to use the weighted PBP in LF as a quantitative index of sympathetic variability in conscious rats, but the evaluation of possible complications controlling the regression parameters is called for.

Action Potentials↗

PubMed2004

Wavelet-based analysis of low-frequency fluctuations of blood pressure and sympathetic nerve activity in rats.

Biorthogonal wavelets were employed to quantify the relationship of fluctuations between blood pressure (BP) and sympathetic nerve activity (SNA). We forced the SNA to fluctuate by electrical stimulation the medulla in anesthetized, paralyzed, vagotomized, cardiac sympathetic-blocked, baroreceptor-denervated, and angiotensin II-converting enzyme-inhibited rats. Although spectral analysis showed a close coupling between fluctuations of BP and SNA at the stimulating frequencies, only the fluctuations of SNA in frequencies of 0.25 to approximately 0.4 Hz were proportional to BP fluctuations over the course of time. The results suggest that fluctuations transmitted from SNA to BP were uniform without shifting due to the nature of vasculature or the lagging of sympathetic action in frequencies of 0.25 to approximately 0.4 Hz, and support the possibility of using low-frequency variabilities of BP to quantitatively estimate fluctuations of SNA at time domain.

Action Potentials↗

PubMed2004

Differential morphine effects on short- and long-latency laser-evoked cortical responses in the rat.

Evoked potential and ensemble neuronal activities were used to study the responses of the primary sensorimotor cortex (SmI) to noxious CO(2) laser irradiation of the middle part of the tail in conscious behaving rats. The hypothesis that systemic morphine treatment preferentially attenuates the longer-latency laser-evoked cortical responses was also tested. Laser-evoked potentials (LEPs) and multiple single-unit (SU) activities were, respectively, recorded from chronically implanted stainless-steel screws and microwire electrodes. When examined individually, many SmI neurons showed either short-latency (<100 ms) or long-latency (300-500 ms) responses to laser irradiation. These neurons are widely dispersed in the tail region and hind limb region of the SmI, and also in the forelimb and head regions of the primary motor cortex (MI). Quantitatively, a higher percentage of neurons in the SmI tail region responded with shorter latencies compared to those in the SmI hind limb region or in the MI. When responses of many simultaneously recorded SU were examined together, short-latency and long-latency SmI ensemble activities matched the LEP1 and LEP2, respectively. Systemic morphine significantly attenuated the long-latency but not the short-latency component in both LEPs as well as ensemble neuronal activity in the tail region of the SmI. These effects were blocked by naloxone pretreatment.

Action Potentials↗

PubMed2003

A simple method for fabricating horizontal and vertical microwire arrays.

Microwire array electrodes are important in multi-site, multiple single-unit recording experiments. A simple method is described herein for the construction of microwire arrays consisting of evenly spaced insulated microwires in either horizontal or vertical orientations. Several key steps in the fabrication of a good microwire array electrode are made easier with this method. These steps include (1) arranging microwires into a desirable configuration, (2) keeping track of microwire sequences, and (3) soldering microwires to closely packed slots. This method needs only general mechanical tools and is relatively simple even for inexperienced workers.

Action Potentials↗